[en] After treatment with methyl or ethyl methane sulfonate, T7 amber mutants display a reduced capacity for recombination. Moreover, alkylation reduces recombination frequency involving markers on the right-hand side of the genetic map more than it reduces recombination frequency involving markers on the left-hand side. We interpret this to mean that alkylation can stop DNA injection at any point along the DNA molecule, and that T7 phage injects its DNA in a unique fashion starting from the end carrying the genes for early proteins.
Disciplines :
Human health sciences: Multidisciplinary, general & others
Author, co-author :
Karska-Wysocki, B
Mamet-Bratley, M D
Verly, Walter ; Relations académiques et scientifiques (Sciences) ; Canada
Language :
English
Title :
Action of ethyl and methyl methane sulfonates on DNA injection and genetic recombination in T7 bacteriophage.
Alternative titles :
[fr] Action des sulfonates d'éthyle et de méthyle méthane sur l'injection d'ADN et la recombinaison génétique dans le bactériophage T7.