Keywords :
DNA, Viral; Mesylates; Ethyl Methanesulfonate; Methyl Methanesulfonate; Alkylation; Coliphages/metabolism; DNA, Viral/biosynthesis; DNA, Viral/metabolism; Ethyl Methanesulfonate/pharmacology; Mesylates/pharmacology; Methyl Methanesulfonate/pharmacology; Mutation; Recombination, Genetic/drug effects; Microbiology; Immunology; Insect Science; Virology
Abstract :
[en] After treatment with methyl or ethyl methane sulfonate, T7 amber mutants display a reduced capacity for recombination. Moreover, alkylation reduces recombination frequency involving markers on the right-hand side of the genetic map more than it reduces recombination frequency involving markers on the left-hand side. We interpret this to mean that alkylation can stop DNA injection at any point along the DNA molecule, and that T7 phage injects its DNA in a unique fashion starting from the end carrying the genes for early proteins.
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