Screening and Characterization of Pediococcus acidilactici LC-9-1 toward Selection as a Potential Probiotic for Poultry with Antibacterial and Antioxidative Properties.
[en] Growing interest has been focused on lactic acid bacteria as alternatives to antimicrobial growth promoters, which are characterized by the production of various functional metabolites, such as antimicrobial and antioxidants compounds. The present study was undertaken to evaluate a potential probiotic from the antioxidant perspective. LC-9-1, screened from the intestines of healthy animals, was revealed to be Pediococcus acidilactici on the basis of its morphological, biochemical, and molecular characteristics. The strain has excellent properties, including acid-production efficiency, antibacterial performance and antioxidant activity. The safety of the strain was also evaluated. Furthermore, the experiments in broiler chickens suggested that dietary LC-9-1 supplementation improved the growth performance and decreased the abdominal fat, and enhanced the antioxidant capability and intestinal innate immunity of broilers. Analysis of intestinal microbiota showed that a higher community diversity (Shannon index) was achieved. In addition to the significantly increased relative abundances of Pediococcus spp., beneficial genera such as Rothia spp. and Ruminococcus spp. were abundant, while opportunistic pathogens such as Escherichia-Shigella spp. were significantly reduced in LC-9-1-supplemented broilers. Collectively, such in-depth characterization and the available data will guide future efforts to develop next-generation probiotics, and LC-9-1 could be considered a potential strain for further utilization in direct-fed microbial or starter culture for fermentation.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Li, Chong ; Université de Liège - ULiège > TERRA Research Centre ; Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, China
Wang, Shaolong; Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, China
Chen, Si; Department of Molecular Cell Biology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Suwon 16419, Republic of Korea
Wang, Xiaoying; Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, China
Deng, Xuejuan; National Engineering Research Center of Biological Feed, Beijing 100081, China
Liu, Guohua ; Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, China
Chang, Wenhuan ; Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, China
Beckers, Yves ; Université de Liège - ULiège > TERRA Research Centre > Animal Sciences (AS)
Cai, Huiyi; Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, China ; National Engineering Research Center of Biological Feed, Beijing 100081, China
Language :
English
Title :
Screening and Characterization of Pediococcus acidilactici LC-9-1 toward Selection as a Potential Probiotic for Poultry with Antibacterial and Antioxidative Properties.
This research was funded by China Agriculture Research System (CARS-41).C.L. gratefully acknowledges financial support from the China Scholarship Council (No. 202103250085).
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Neveling D.P. Van Emmenes L. Ahire J.J. Pieterse E. Smith C. Dicks L.M.T. Safety Assessment of Antibiotic and Probiotic Feed Additives for Gallus gallus Domesticus Sci. Rep. 2017 7 12767 10.1038/s41598-017-12866-7
Mishra B. Jha R. Oxidative Stress in the Poultry Gut: Potential Challenges and Interventions Front. Vet. Sci. 2019 6 60 10.3389/fvets.2019.00060 30886854
Pasolli E. De Filippis F. Mauriello I.E. Cumbo F. Walsh A.M. Leech J. Cotter P.D. Segata N. Ercolini D. Large-Scale Genome-Wide Analysis Links Lactic Acid Bacteria from Food with the Gut Microbiome Nat. Commun. 2020 11 2610 10.1038/s41467-020-16438-8 32451391
Pan D. Mei X. Antioxidant Activity of an Exopolysaccharide Purified from Lactococcus lactis Subsp. lactis 12 Carbohydr. Polym. 2010 80 908 914 10.1016/j.carbpol.2010.01.005
Bajpai V.K. Han J.H. Rather I.A. Park C. Lim J. Paek W.K. Lee J.S. Yoon J.I. Park Y.H. Characterization and Antibacterial Potential of Lactic Acid Bacterium Pediococcus pentosaceus 4I1 Isolated from Freshwater Fish Zacco koreanus Front. Microbiol. 2016 7 2037 10.3389/fmicb.2016.02037 28066360
Covention on Biological Diversity The Nagoya Protocol on Access and Benefit-Sharing Available online: https://www.cbd.int/abs/ (accessed on 7 July 2022)
Pan F. Zhang L. Li M. Hu Y. Zeng B. Yuan H. Zhao L. Zhang C. Predominant Gut Lactobacillus murinus Strain Mediates Anti-Inflammaging Effects in Calorie-Restricted Mice Microbiome 2018 6 54 10.1186/s40168-018-0440-5 29562943
Cho Y.H. Hong S.M. Kim C.H. Isolation and Characterization of Lactic Acid Bacteria from Kimchi, Korean Traditional Fermented Food to Apply into Fermented Dairy Products Food Sci. Anim. Resour. 2013 33 75 82 10.5851/kosfa.2013.33.1.75
Xiao P. Huang Y. Yang W. Zhang B. Quan X. Screening Lactic Acid Bacteria with High Yielding-Acid Capacity from Pickled Tea for Their Potential Uses of Inoculating to Ferment Tea Products J. Food Sci. Technol. 2015 52 6727 6734 10.1007/s13197-015-1803-6
Melgaço A.C.C. Pessoa W.F.B. Freire H.P. De Almeida M.E. Barbosa M.S. Rezende R.P. Timenetsky J. Marques L.M. Romano C.C. Potential of Maintaining a Healthy Vaginal Environment by Two Lactobacillus Strains Isolated from Cocoa Fermentation BioMed Res. Int. 2018 2018 7571954 10.1155/2018/7571954
Lin X. Xia Y. Wang G. Yang Y. Xiong Z. Lv F. Zhou W. Ai L. Lactic Acid Bacteria with Antioxidant Activities Alleviating Oxidized Oil Induced Hepatic Injury in Mice Front. Microbiol. 2018 9 2684 10.3389/fmicb.2018.02684
Li S. Zhao Y. Zhang L. Zhang X. Huang L. Li D. Niu C. Yang Z. Wang Q. Antioxidant Activity of Lactobacillus plantarum Strains Isolated from Traditional Chinese Fermented Foods Food Chem. 2012 135 1914 1919 10.1016/j.foodchem.2012.06.048 22953940
Kobierecka P.A. Wyszyńska A.K. Aleksandrzak-Piekarczyk T. Kuczkowski M. Tuzimek A. Piotrowska W. Górecki A. Adamska I. Wieliczko A. Bardowski J. et al. In Vitro Characteristics of Lactobacillus Spp. Strains Isolated from the Chicken Digestive Tract and Their Role in the Inhibition of Campylobacter Colonization Microbiologyopen 2017 6 e00512 10.1002/mbo3.512 28736979
Wang Y. Li A. Jiang X. Zhang H. Mehmood K. Zhang L. Jiang J. Waqas M. Iqbal M. Li J. Probiotic Potential of Leuconostoc Pseudomesenteroides and Lactobacillus Strains Isolated From Yaks Front. Microbiol. 2018 9 2987 10.3389/fmicb.2018.02987 30564222
Bajpai V.K. Han J.H. Nam G.J. Majumder R. Park C. Lim J. Paek W.K. Rather I.A. Park Y.H. Characterization and Pharmacological Potential of Lactobacillus Sakei 1I1 Isolated from Fresh Water Fish Zacco Koreanus DARU J. Pharm. Sci. 2016 24 8 10.1186/s40199-016-0147-8 26980217
Fitzgerald S. Duffy E. Holland L. Morrin A. Multi-Strain Volatile Profiling of Pathogenic and Commensal Cutaneous Bacteria Sci. Rep. 2020 10 17971 10.1038/s41598-020-74909-w
Zhang B. Wang Y. Tan Z. Li Z. Jiao Z. Huang Q. Screening of Probiotic Activities of Lactobacilli Strains Isolated from Traditional Tibetan Qula, A Raw Yak Milk Cheese Asian-Australas. J. Anim. Sci. 2016 29 1490 10.5713/ajas.15.0849
Maragkoudakis P.A. Zoumpopoulou G. Miaris C. Kalantzopoulos G. Pot B. Tsakalidou E. Probiotic Potential of Lactobacillus Strains Isolated from Dairy Products Int. Dairy J. 2006 16 189 199 10.1016/j.idairyj.2005.02.009
CLSI Performance Standards For Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement CLSI Document M100-S25 Clinical Laboratory Standards Institute Wayne, PA, USA 2015
Reuben R.C. Roy P.C. Sarkar S.L. Alam R.U. Jahid I.K. Isolation, Characterization, and Assessment of Lactic Acid Bacteria toward Their Selection as Poultry Probiotics BMC Microbiol. 2019 19 253 10.1186/s12866-019-1626-0
Nikoskelainen S. Ouwehand A.C. Bylund G. Salminen S. Lilius E.M. Immune Enhancement in Rainbow Trout (Oncorhynchus mykiss) by Potential Probiotic Bacteria (Lactobacillus rhamnosus) Fish Shellfish Immunol. 2003 15 443 452 10.1016/S1050-4648(03)00023-8
Kilkenny C. Browne W.J. Cuthi I. Emerson M. Altman D.G. Improving Bioscience Research Reporting: The ARRIVE Guidelines for Reporting Animal Research Vet. Clin. Pathol. 2012 41 27 31 10.1016/j.joca.2012.02.010
Liu T. Li C. Zhong H. Feng F. Dietary Medium-Chain α-Monoglycerides Increase BW, Feed Intake, and Carcass Yield in Broilers with Muscle Composition Alteration Poult. Sci. 2021 100 186 10.1016/j.psj.2020.09.056 33357680
Gao J. Wang R. Liu J. Wang W. Chen Y. Cai W. Effects of Novel Microecologics Combined with Traditional Chinese Medicine and Probiotics on Growth Performance and Health of Broilers Poult. Sci. 2022 101 101412 10.1016/j.psj.2021.101412 34920387
Salem R. El-Habashi N. Fadl S.E. Sakr O.A. Elbialy Z.I. Effect of Probiotic Supplement on Aflatoxicosis and Gene Expression in the Liver of Broiler Chicken Environ. Toxicol. Pharmacol. 2018 60 118 127 10.1016/j.etap.2018.04.015 29705371
Wang J. Clark D.L. Jacobi S.K. Velleman S.G. Effect of Vitamin E and Alpha Lipoic Acid on Intestinal Development Associated with Wooden Breast Myopathy in Broilers Poult. Sci. 2021 100 100952 10.1016/j.psj.2020.12.049 33652526
Lozupone C. Knight R. UniFrac: A New Phylogenetic Method for Comparing Microbial Communities Appl. Environ. Microbiol. 2005 71 8228 8235 10.1128/AEM.71.12.8228-8235.2005 16332807
Segata N. Izard J. Waldron L. Gevers D. Miropolsky L. Garrett W.S. Huttenhower C. Metagenomic Biomarker Discovery and Explanation Genome Biol. 2011 12 R60 10.1186/gb-2011-12-6-r60 21702898
Huang P. Zhang Y. Xiao K. Jiang F. Wang H. Tang D. Liu D. Liu B. Liu Y. He X. et al. The Chicken Gut Metagenome and the Modulatory Effects of Plant-Derived Benzylisoquinoline Alkaloids Microbiome 2018 6 1 17 10.1186/s40168-018-0590-5
Abbasiliasi S. Tan J.S. Bashokouh F. Ibrahim T.A.T. Mustafa S. Vakhshiteh F. Sivasamboo S. Ariff A.B. In Vitro Assessment of Pediococcus Acidilactici Kp10 for Its Potential Use in the Food Industry BMC Microbiol. 2017 17 121 10.1186/s12866-017-1000-z
Ragaa N.M. Korany R.M.S. Studying the Effect of Formic Acid and Potassium Diformate on Performance, Immunity and Gut Health of Broiler Chickens Anim. Nutr. 2016 2 296 10.1016/j.aninu.2016.08.003
Bao J. Zhang Y. Zhang L. Gong X. Shi W. Liu L. Wang X. Therapeutic Effect of Schisandrin A on Avian Colibacillosis through Gut-Liver Axis Poult. Sci. 2021 100 101371 10.1016/j.psj.2021.101371
Zhou C. Liang J. Jiang W. He X. Liu S. Wei P. The Effect of a Selected Yeast Fraction on the Prevention of Pullorum Disease and Fowl Typhoid in Commercial Breeder Chickens Poult. Sci. 2020 99 101 10.3382/ps/pez567
Rostami K. Nemati M. Pourahmad F. Prophage Typing of Staphylococcus Aureus Strains Isolated from Broiler Poultry Arch. Razi Inst. 2021 76 499 506 10.22092/ARI.2020.343199.1498 34824743
Li Z. Peng C. Zhang G. Shen Y. Zhang Y. Liu C. Liu M. Wang F. Prevalence and Characteristics of Multidrug-Resistant Proteus mirabilis from Broiler Farms in Shandong Province, China Poult. Sci. 2022 101 101710 10.1016/j.psj.2022.101710
da Silva Frasao B. dos Santos Rosario A.I.L. Leal Rodrigues B. Bitti H.A. Baltar J.D. Nogueira R.I. da Costa M.P. Conte-Junior C.A. Impact of Juçara (Euterpe edulis) Fruit Waste Extracts on the Quality of Conventional and Antibiotic-Free Broiler Meat Poult. Sci. 2021 100 101232 10.1016/j.psj.2021.101232 34225206
Ehrmann M.A. Kurzak P. Bauer J. Vogel R.F. Characterization of Lactobacilli towards Their Use as Probiotic Adjuncts in Poultry J. Appl. Microbiol. 2002 92 966 975 10.1046/j.1365-2672.2002.01608.x
Köberl M. Erschen S. Etemadi M. White R.A. El-Arabi T.F. Berg G. Deciphering the Microbiome Shift during Fermentation of Medicinal Plants Sci. Rep. 2019 9 13461 10.1038/s41598-019-49799-2
Campedelli I. Mathur H. Salvetti E. Clarke S. Rea M.C. Torriani S. Ross R.P. Hill C. O’Toole P.W. Genus-Wide Assessment of Antibiotic Resistance in Lactobacillus Spp. Appl. Environ. Microbiol. 2019 85 e01738-18 10.1128/AEM.01738-18
Jazi V. Foroozandeh A.D. Dastar B. Rezaie Koochaksaraie R. Toghyani M. Effects of Pediococcus Acidilactici, Mannan-Oligosaccharide, Butyric Acid and Their Combination on Growth Performance and Intestinal Health in Young Broiler Chickens Challenged with Salmonella Typhimurium Poult. Sci. 2018 97 2034 2043 10.3382/ps/pey035
Wu Y. Lei Z. Wang Y. Yin D. Aggrey S.E. Guo Y. Yuan J. Metabolome and Microbiota Analysis Reveals the Conducive Effect of Pediococcus acidilactici BCC-1 and Xylan Oligosaccharides on Broiler Chickens Front. Microbiol. 2021 12 1337 10.3389/fmicb.2021.683905
Chen J. Ren X. Li L. Lu S. Chen T. Tan L. Liu M. Luo Q. Liang S. Nie Q. et al. Integrative Analyses of MRNA Expression Profile Reveal the Involvement of IGF2BP1 in Chicken Adipogenesis Int. J. Mol. Sci. 2019 20 2923 10.3390/ijms20122923
Alhidary I.A. Abdelrahman M.M. Khan U.R. Harron M. Abdelrahman M. Uallh Khan R. Harron R.M. Antioxidant Status and Immune Responses of Growing Camels Supplemented a Long-Acting Multi-Trace Minerals Rumen Bolus Ital. J. Anim. Sci. 2016 15 343 349 10.1080/1828051X.2016.1186502
Estévez M. Oxidative Damage to Poultry: From Farm to Fork Poult. Sci. 2015 94 1368 1378 10.3382/ps/pev094
Shields S. Greger M. Animal Welfare and Food Safety Aspects of Confining Broiler Chickens to Cages Animals 2013 3 386 400 10.3390/ani3020386 26487409
Li K. Fu W. Bo Y. Zhu Y. Effect of Albumin-Globulin Score and Albumin to Globulin Ratio on Survival in Patients with Heart Failure: A Retrospective Cohort Study in China BMJ Open 2018 8 e022960 10.1136/bmjopen-2018-022960 29982222
Gabay C. Kushner I. Acute-Phase Proteins and Other Systemic Responses to Inflammation N. Engl. J. Med. 1999 340 448 454 10.1056/NEJM199902113400607 9971870
Son J. Kim H.J. Hong E.C. Kang H.K. Effects of Stocking Density on Growth Performance, Antioxidant Status, and Meat Quality of Finisher Broiler Chickens under High Temperature Antioxidants 2022 11 871 10.3390/antiox11050871
Estevez M. Petracci M. Benefits of Magnesium Supplementation to Broiler Subjected to Dietary and Heat Stress: Improved Redox Status, Breast Quality and Decreased Myopathy Incidence Antioxidants 2019 8 456 10.3390/antiox8100456
Andreadis C. Gimotty P.A. Wahl P. Hammond R. Houldsworth J. Schuster S.J. Rebbeck T.R. Members of the Glutathione and ABC-Transporter Families Are Associated with Clinical Outcome in Patients with Diffuse Large B-Cell Lymphoma Blood 2007 109 3409 10.1182/blood-2006-09-047621
Giustarini D. Dalle-Donne I. Tsikas D. Rossi R. Oxidative Stress and Human Diseases: Origin, Link, Measurement, Mechanisms, and Biomarkers Crit. Rev. Clin. Lab. Sci. 2009 46 241 281 10.3109/10408360903142326 19958214
Shen Y. Fan X. Zhang L. Wang Y. Li C. Lu J. Zha B. Wu Y. Chen X. Zhou J. et al. Thresholds of Glycemia and the Outcomes of COVID-19 Complicated With Diabetes: A Retrospective Exploratory Study Using Continuous Glucose Monitoring Diabetes Care 2021 44 976 10.2337/dc20-1448 33574126
Daneshmand A. Kermanshahi H. Sekhavati M.H. Javadmanesh A. Ahmadian M. Antimicrobial Peptide, CLF36, Affects Performance and Intestinal Morphology, Microflora, Junctional Proteins, and Immune Cells in Broilers Challenged with E. Coli Sci. Rep. 2019 9 14176 10.1038/s41598-019-50511-7
Inatomi T. Otomaru K. Effect of Dietary Probiotics on the Semen Traits and Antioxidative Activity of Male Broiler Breeders Sci. Rep. 2018 8 5874 10.1038/s41598-018-24345-8
Dong Y. Li R. Liu Y. Ma L. Zha J. Qiao X. Chai T. Wu B. Benefit of Dietary Supplementation with Bacillus Subtilis BYS2 on Growth Performance, Immune Response, and Disease Resistance of Broilers Probiotics Antimicrob. Proteins 2020 12 1385 1397 10.1007/s12602-020-09643-w
Liew W.P.P. Mohd-Redzwan S. Than L.T.L. Gut Microbiota Profiling of Aflatoxin B1-Induced Rats Treated with Lactobacillus casei Shirota Toxins 2019 11 49 10.3390/toxins11010049
Jung Y.S. Wang W. Jun S. Zhang J. Srivastava M. Kim M.J. Lien E.M. Shang J. Chen J. McCrea P.D. et al. Deregulation of CRAD-Controlled Cytoskeleton Initiates Mucinous Colorectal Cancer via β-Catenin Nat. Cell Biol. 2018 20 1303 10.1038/s41556-018-0215-z
Hong Y. Cheng Y. Li Y. Li X. Zhou Z. Shi D. Li Z. Xiao Y. Preliminary Study on the Effect of Bacillus Amyloliquefaciens TL on Cecal Bacterial Community Structure of Broiler Chickens BioMed Res. Int. 2019 10 5431354 10.1155/2019/5431354
Konstantinov S.R. Favier C.F. Zhu W.Y. Williams B.A. Klüß J. Souffrant W.B. De Vos W.M. Akkermans A.D.L. Smidt H. Microbial Diversity Studies of the Porcine Gastrointestinal Ecosystem during Weaning Transition Anim. Res. 2004 53 317 324 10.1051/animres:2004019
Guo S. Xi Y. Xia Y. Wu T. Zhao D. Zhang Z. Ding B. Dietary Lactobacillus fermentum and Bacillus Coagulans Supplementation Modulates Intestinal Immunity and Microbiota of Broiler Chickens Challenged by Clostridium perfringens Front. Vet. Sci. 2021 8 483 10.3389/fvets.2021.680742
Mora A. Herrera A. Mamani R. López C. Alonso M.P. Blanco J.E. Blanco M. Dahbi G. García-Garrote F. Pita J.M. et al. Recent Emergence of Clonal Group O25b:K1:H4-B2-ST131 IbeA Strains among Escherichia Coli Poultry Isolates, Including CTX-M-9-Producing Strains, and Comparison with Clinical Human Isolates Appl. Environ. Microbiol. 2010 76 6991 6997 10.1128/AEM.01112-10 20817805
Yang W.Y. Lee Y. Lu H. Chou C.H. Wang C. Analysis of Gut Microbiota and the Effect of Lauric Acid against Necrotic Enteritis in Clostridium Perfringens and Eimeria Side-by-Side Challenge Model PLoS ONE 2019 14 e0205784 10.1371/journal.pone.0205784 31150394
Araos R. Andreatos N. Ugalde J. Mitchell S. Mylonakis E. D’Agata E.M.C. Fecal Microbiome Among Nursing Home Residents with Advanced Dementia and Clostridium Difficile Dig. Dis. Sci. 2018 63 1525 1531 10.1007/s10620-018-5030-7 29594967
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.