Virology; Infectious Diseases; Cyprinid Herpesvirus 2; Alloherpesviridae; Cyprinivirus; Carassius auratus; aquaculture; pathogenesis; portal of entry; recombinant viruses; in vivo imaging
Abstract :
[en] Cyprinid herpesvirus 2 (CyHV-2) is a virus that causes mass mortality in economically important Carassius spp. However, there have been no comprehensive studies into host susceptibility or permissivity with respect to developmental stage, and the major portal of viral entry into the host is still unclear. To help bridge these knowledge gaps, we developed the first ever recombinant strain of CyHV-2 expressing bioluminescent and fluorescent reporter genes. Infection of Carassius auratus hosts with this recombinant by immersion facilitated the exploitation of various in vivo imaging techniques to establish the spatiotemporal aspects of CyHV-2 replication at larval, juvenile, and adult developmental stages. While less susceptible than later developmental stages, larvae were most permissive to CyHV-2 replication, leading to rapid systemic infection and high mortality. Permissivity to CyHV-2 decreased with advancing development, with adults being the least permissive and, thus, also exhibiting the least mortality. Across all developmental stages, the skin was the most susceptible and permissive organ to infection at the earliest sampling points post-infection, indicating that it represents the major portal of entry into these hosts. Collectively these findings provide important fundamental insights into CyHV-2 pathogenesis and epidemiology in Carassius auratus with high relevance to other related economically important virus-host models.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
He, Bo ✱; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH)
Sridhar, Arun ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Streiff, Cindy ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH)
Deketelaere, Caroline ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI)
Zhang, Haiyan ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Gao, Yuan; Immunology-Vaccinology, Department of Infectious and Parasitic Diseases, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium
Hu, Yunlong ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Pirotte, Sébastien ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Delrez, Natacha ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Davison, Andrew J. ; MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK
Donohoe, Owen ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire ; Bioscience Research Institute, Technological University of the Shannon, Athlone N37 HD68, Co. Westmeath, Ireland
Vanderplasschen, Alain ✱; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Immunologie vétérinaire
✱ These authors have contributed equally to this work.
Language :
English
Title :
In Vivo Imaging Sheds Light on the Susceptibility and Permissivity of Carassius auratus to Cyprinid Herpesvirus 2 According to Developmental Stage
Publication date :
15 August 2023
Journal title :
Viruses
eISSN :
1999-4915
Publisher :
MDPI AG
Volume :
15
Issue :
8
Pages :
1746
Peer reviewed :
Peer Reviewed verified by ORBi
Tags :
CÉCI : Consortium des Équipements de Calcul Intensif
ULiège - University of Liège F.R.S.-FNRS - Fonds de la Recherche Scientifique EMFF - European Maritime and Fisheries Fund WELBIO - Walloon Excellence in Life Sciences and Biotechnology Walloon region MRC - Medical Research Council
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