Capsid Proteins; RNA, Messenger; RNA, Viral; RNA; Capsid Proteins/genetics; Genome, Viral; High-Throughput Nucleotide Sequencing; Open Reading Frames; Phylogeny; Plant Diseases; RNA, Viral/genetics; Reunion; Ananas; Flexiviridae/genetics; Flexiviridae; Virology; General Medicine
Abstract :
[en] We report the complete genome sequence of a novel member of the genus Vitivirus (family Betaflexiviridae, subfamily Trivirinae) infecting pineapple. The complete genome sequence of this virus was obtained from total RNA extracted from pineapple leaf samples collected in Reunion Island, using a combination of high-throughput sequencing technologies. The viral genome is 6,757 nt long, excluding the poly(A) tail, and shares all the hallmarks of vitiviruses. Phylogenetic analysis performed on the replication-associated protein and capsid protein gene sequences unambiguously place this new virus, for which we propose the name "pineapple virus A", in the genus Vitivirus.
Disciplines :
Biotechnology
Author, co-author :
Massé, Delphine; ANSES, LSV RAPT, 97410, Saint Pierre, La Réunion, France ; Université de La Réunion, UMR PVBMT, 97410, Saint Pierre, La Réunion, France
Filloux, Denis; UMR PHIM, CIRAD, Campus International de Baillarguet, 34398, Montpellier Cedex-5, France ; PHIM Plant Health Institute, Univ Montpellier, CIRAD, INRAE, Institut Agro, IRD, 34090, Montpellier, France
Candresse, Thierry; Univ. Bordeaux, INRAE, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882, Villenave d'Ornon Cedex, France
Massart, Sébastien ; Université de Liège - ULiège > TERRA Research Centre > Gestion durable des bio-agresseurs
Marais, Armelle; Univ. Bordeaux, INRAE, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882, Villenave d'Ornon Cedex, France
Verdin, Eric; INRAE, UR407 Unité de Pathologie Végétale, CS 60094, 84140, Montfavet, France
Cassam, Nathalie; ANSES, LSV RAPT, 97410, Saint Pierre, La Réunion, France
Fernandez, Emmanuel; UMR PHIM, CIRAD, Campus International de Baillarguet, 34398, Montpellier Cedex-5, France ; PHIM Plant Health Institute, Univ Montpellier, CIRAD, INRAE, Institut Agro, IRD, 34090, Montpellier, France
Roumagnac, Philippe; UMR PHIM, CIRAD, Campus International de Baillarguet, 34398, Montpellier Cedex-5, France ; PHIM Plant Health Institute, Univ Montpellier, CIRAD, INRAE, Institut Agro, IRD, 34090, Montpellier, France
Teycheney, Pierre-Yves; CIRAD, UMR PVBMT, 97410, Saint Pierre, La Réunion, France
Lefeuvre, Pierre; CIRAD, UMR PVBMT, 97410, Saint Pierre, La Réunion, France
Lett, Jean-Michel ; CIRAD, UMR PVBMT, 97410, Saint Pierre, La Réunion, France. lett@cirad.fr
Language :
English
Title :
Identification of a novel vitivirus from pineapple in Reunion Island.
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