High Prevalence of blaNDM Among Carbapenem Non‑Susceptible Klebsiella pneumoniae in a Tunisian Hospital First Report of blaNDM‑9, blaKPC‑20, and blaKPC‑26 Genes
[en] Fifty-four carbapenem non-susceptible Klebsiella pneumoniae (CNSKP) isolates were collected from a Tunisian hospital over a period of 13 consecutive months. Carbapenemase production and the prevalence of carbapenemase-encoding genes
were investigated using combined-disk test (CDT), modified Carba NP (mCarba NP) test, and UV-spectrophotometry method complemented by PCR experiments and sequencing. Carbapenemase production was detected by the mCarba NP test and CDT in 92.59% and 96.29% of the 54 CNSKP isolates, respectively; while imipenem hydrolysis was detected using UV-spectrophotometry in the crude extracts of 44 isolates. blaNDM, blaOXA-48-like , and blaKPC carbapenemase-encoding genes were found in 48, 31, and 22 isolates, respectively. Remarkably, blaNDM-9, blaKPC-20 , and blaKPC-26 genes were reported. The
co-occurrence of carbapenemase-encoding genes in a single isolate was detected in 62.96% of the isolates. The analysis of clonal relationships between the isolates by pulsed field gel electrophoresis revealed that the majority of them were geneti-cally unrelated. Our investigation provides molecular data on enzymatic mechanism of carbapenem non-susceptibility among 54 CNSKP showing the dominance of blaNDM, and comprises the first identification of blaNDM-9, blaKPC-20 , and blaKPC-26 genes in a Tunisia hospital.
Disciplines :
Microbiology
Author, co-author :
Zouaoui, Emna; Biochemistry and Biotechnology Laboratory LR01ES05, Faculty of Sciences of Tunis, University Tunis El Manar, 2092 El Manar II, Tunisia
Mercuri, Paola ; Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Radaoui, Anis; Research Laboratory LR18ES39, Faculty of Medicine of Tunis, University of Tunis El Manar, 2092 El Manar II
Ben Salah, Naouel; Laboratory of Clinical Biology, Regional Hospital of Ben Arous, Medina Jadida 3, 2096 Ben Arous, Tunisia
Galleni, Moreno ; Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Ben-Mahrez, Kamel; Biochemistry and Biotechnology Laboratory LR01ES05, Faculty of Sciences of Tunis, University Tunis El Manar, 2092 El Manar II, Tunisia
Réjiba, Samia; Biochemistry and Biotechnology Laboratory LR01ES05, Faculty of Sciences of Tunis, University Tunis El Manar, 2092 El Manar II, Tunisia
Language :
English
Title :
High Prevalence of blaNDM Among Carbapenem Non‑Susceptible Klebsiella pneumoniae in a Tunisian Hospital First Report of blaNDM‑9, blaKPC‑20, and blaKPC‑26 Genes
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