Article (Scientific journals)
Potentials of a Plasma-Aerosol System for Wound Healing Advanced by Drug Introduction: An In Vitro Study.
Sremački, Ivana; Asadian, Mahtab; De Geyter, Nathalie et al.
2023In ACS Biomaterial Science and Engineering, 9 (5), p. 2392 - 2407
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Keywords :
FTIC dextran; biomedical application; cold plasma; drug delivery; wound healing; Reactive Oxygen Species; Hydrogen Peroxide; Collagen; Reactive Nitrogen Species; Aerosols; Reactive Oxygen Species/metabolism; Collagen/pharmacology; Reactive Nitrogen Species/pharmacology; Aerosols/pharmacology; Hydrogen Peroxide/pharmacology; Wound Healing; Aerosol systems; Biomedical applications; Biomedical fields; Cold plasmas; In-vitro; Plasma treatment; Vitro studies; Biomaterials; Biomedical Engineering
Abstract :
[en] Cold plasmas have found their application in a wide range of biomedical fields by virtue of their high chemical reactivity. In the past decades, many attempts have been made to use cold plasmas in wound healing, and within this field, many studies have focused on plasma-induced cell proliferation mechanisms. In this work, one step further has been taken to demonstrate the advanced role of plasma in wound healing. To this end, the simultaneous ability of plasma to induce cell proliferation and permeabilize treated cells has been examined in the current study. The driving force was to advance the wound healing effect of plasma with drug delivery. On this subject, we demonstrate in vitro the healing effect of Ar, Ar+N2 plasma, and their aerosol counterparts. A systematic study has been carried out to study the role of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in cell adhesion, signaling, differentiation, and proliferation. An additional investigation was also performed to study the permeabilization of cells and the delivery of the modeled drug carrier fluorescein isothiocyanate (FITC) labeled dextran into cells upon plasma treatment. Short 35 s plasma treatments were found to promote fibroblast adhesion, migration, signaling, proliferation, and differentiation by means of reactive oxygen and nitrogen species (RONS) created by plasma and deposited into the cell environment. The impact of the plasma downstream products NO2- and NO3- on the expressions of the focal adhesion's genes, syndecans, and collagens was observed to be prominent. On the other hand, the differentiation of fibroblasts to myofibroblasts was mainly initiated by ROS produced by the plasma. In addition, the ability of plasma to locally permeabilize fibroblast cells was demonstrated. During proliferative cell treatment, plasma can simultaneously induce cell membrane permeabilization (d ∼ 7.3 nm) by the species OH and H2O2. The choice for a plasma or a plasma-aerosol configuration thus allows the possibility to change the spatial chemistry of drug delivery molecules and thus to locally deliver drugs. Accordingly, this study offers a pivotal step toward plasma-assisted wound healing advanced by drug delivery.
Disciplines :
Engineering, computing & technology: Multidisciplinary, general & others
Author, co-author :
Sremački, Ivana ;  Department of Applied Physics, Ghent University, Sint-Pietersnieuwstraat 41, Gent 9000, Belgium
Asadian, Mahtab ;  Department of Applied Physics, Ghent University, Sint-Pietersnieuwstraat 41, Gent 9000, Belgium ; Skeletal Biology & Engineering Research Center, ON1 Herestraat 49, 3000 Leuven, Belgium
De Geyter, Nathalie;  Department of Applied Physics, Ghent University, Sint-Pietersnieuwstraat 41, Gent 9000, Belgium
Leys, Christophe;  Department of Applied Physics, Ghent University, Sint-Pietersnieuwstraat 41, Gent 9000, Belgium
Geris, Liesbet  ;  Université de Liège - ULiège > Département d'aérospatiale et mécanique > Génie biomécanique ; Skeletal Biology & Engineering Research Center, ON1 Herestraat 49, 3000 Leuven, Belgium
Nikiforov, Anton ;  Department of Applied Physics, Ghent University, Sint-Pietersnieuwstraat 41, Gent 9000, Belgium
Language :
English
Title :
Potentials of a Plasma-Aerosol System for Wound Healing Advanced by Drug Introduction: An In Vitro Study.
Publication date :
08 May 2023
Journal title :
ACS Biomaterial Science and Engineering
eISSN :
2373-9878
Publisher :
American Chemical Society, United States
Volume :
9
Issue :
5
Pages :
2392 - 2407
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FWO - Fonds Wetenschappelijk Onderzoek Vlaanderen [BE]
Funding text :
This study was performed as a collaboration between Ghent University and KU Leuven and was supported by the FWO project “Plasma-skin interactions: from wound treatment to topical introduction of molecules”, number G084917N.
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since 06 July 2023

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