A method for rapid and homogenous initiation of post-harvest physiological deterioration in cassava storage roots identifies Indonesian cultivars with improved shelf-life performance.
Zainuddin, Ima M; Lecart, Brieuc; Sudarmonowati, Ennyet al.
[en] Cassava is the most cultivated and consumed root crop in the world. One of the major constraints to the cassava value chain is the short shelf life of cassava storage roots which is primarily due to the so-called post-harvest physiological deterioration (PPD). The identification of natural sources of PPD tolerance represents a key approach to mitigating PPD losses by generating farmer- and industry-preferred cassava cultivars with prolonged shelf life. In the present study, a PPD assessment method was developed to screen for PPD tolerance in the cassava germplasm. The proposed PPD assessment method displayed a reduced rate of microbial infection and allowed a rapid and homogenous development of typical PPD symptoms in the cassava storage roots. We successfully used the PPD assessment method in combination with an image-based PPD scoring method to identify and characterize PPD tolerance in 28 cassava cultivars from the Indonesian cassava germplasm. Our analysis showed a significant and positive correlation between PPD score and dry matter content (r = 0.589-0.664, p-value < 0.001). Analysis of additional root parameters showed a significant and positive correlation between PPD scores at 2 days post-harvest (dph) and root length (r = 0.388, p-value < 0.05). Our analysis identified at least 4 cultivars displaying a significantly delayed onset of PPD symptoms as compared to the other selected cultivars. The availability of cassava cultivars contrasting for tolerance to PPD will be particularly instrumental to understanding the molecular mechanisms associated with delayed PPD in cassava roots.
Zainuddin, Ima M; Department of Biology, Plant Biotechnology, Eidgenössische Technische Hochschule (ETH) Zurich, Universitätstrasse 2, 8092, Zurich, Switzerland. ima.zainuddin@kuleuven.be ; Department of Biosystems, KU Leuven, Willem de Croylaan 42, Box 2455, 3001, Louvain, Belgium. ima.zainuddin@kuleuven.be ; Institut Teknologi Bandung (ITB), Jl. Ganesha 10, Bandung, 40132, Indonesia. ima.zainuddin@kuleuven.be ; Research Center for Genetics Engineering, National Research and Innovation Agency (BRIN), Jl. Raya Bogor Km. 46, Cibinong, 16911, Indonesia. ima.zainuddin@kuleuven.be
Lecart, Brieuc ; Université de Liège - ULiège > TERRA Research Centre
Sudarmonowati, Enny; Research Center for Genetics Engineering, National Research and Innovation Agency (BRIN), Jl. Raya Bogor Km. 46, Cibinong, 16911, Indonesia
Vanderschuren, Hervé ; Université de Liège - ULiège > TERRA Research Centre > Plant Sciences ; Department of Biology, Plant Biotechnology, Eidgenössische Technische Hochschule (ETH) Zurich, Universitätstrasse 2, 8092, Zurich, Switzerland. herve.vanderschuren@kuleuven.be ; Department of Biosystems, KU Leuven, Willem de Croylaan 42, Box 2455, 3001, Louvain, Belgium. herve.vanderschuren@kuleuven.be
Language :
English
Title :
A method for rapid and homogenous initiation of post-harvest physiological deterioration in cassava storage roots identifies Indonesian cultivars with improved shelf-life performance.
This work was supported by the Sawiris Foundation for Social Development through Engineering for Development (E4D) doctoral scholarship program (ETH Global) and P3MI ITB fund to I.M.Z. as well as by a FOSC ERA-NET grant (PHEALING).We thank Prof. Wilhelm Gruissem for access to laboratory facilities at ETHZ and Sarah Grimm from Seminar for Statistics ETHZ for valuable statistical consultation. We thank Dr. Sri Hartati, Ibu Hartati, Nawawi and the team from BRIN for their help in the whole process during the PPD assessment.
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