Article (Scientific journals)
Engineering bone-forming biohybrid sheets through the integration of melt electrowritten membranes and cartilaginous microspheroids.
Hall, Gabriella Nilsson; Chandrakar, Amit; Pastore, Angela et al.
2022In Acta Biomaterialia
Peer Reviewed verified by ORBi
 

Files


Full Text
ActaBiomat_2023_Hall.pdf
Publisher postprint (4 MB) Creative Commons License - Public Domain Dedication
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Melt electrowriting; developmental engineering; endochondral ossification; spheroids
Abstract :
[en] Bone fractures are one of the most common traumatic large-organ injuries and although many fractures can heal on their own, 2-12% of fractures are slow healing or do not heal (nonunions). Autologous grafts are currently used for treatment of nonunions but are associated with limited healthy bone tissue. Tissue engineered cell-based products have promise for an alternative treatment method. It was previously demonstrated that cartilaginous microspheroids of periosteum-derived cells could be assembled into scaffold-free constructs and heal murine critically-sized long bone defects (nonunions). However, the handleability of such scaffold-free implants can be compromised when scaling-up. In this work, cartilaginous spheroids were combined with melt electrowritten (MEW) meshes to create an engineered cell-based implant, able to induce in vivo bone formation. MEW polycaprolactone meshes were tailored to contain pores (116 ± 28 µm) of a size that captured microspheroids (180 ± 15 µm). Periosteum-derived microspheroids pre-cultured for 4 days, were seeded on MEW meshes and gene expression analysis demonstrated up-regulation of chondrogenic (SOX9, COL2) and prehypertrophic (VEGF) gene markers after 14 days, creating a biohybrid sheet. When implanted subcutaneously (4 weeks), the biohybrid sheets mineralized (23 ± 3 % MV/TV) and formed bone and bone marrow. Bone formation was also observed when implanted in a murine critically-sized long bone defect, though a high variation between samples was detected. The high versatility of this biofabrication approach lies in the possibility to tailor the scaffolds to shape and dimensions corresponding to the large bone defects and the individual patient using robust bone forming building blocks. These strategies are instrumental in the development of personalized regenerative therapies with predictive clinical outcomes. STATEMENT OF SIGNIFICANCE: : Successful treatments for healing of large long bone defects are still limited and 2-12% of fractures do not heal properly. We combined a novel biofabrication technique: melt electrowriting (MEW), with robust biology: bone forming cartilaginous spheroids to create biohybrid sheets able to form bone upon implantation. MEW enabled the fabrication of scaffolds with micrometer-sized fibers in defined patterns which allowed the capturing of and merging with cartilaginous spheroids which had the potency to mature into bone via the developmental process of endochondral ossification. The present study contributes to the rapidly growing field of "Biofabrication with Spheroid and Organoid Materials'' and demonstrates design considerations that are of great importance for biofabrication of functional tissues through the assembly of cellular spheroids.
Disciplines :
Engineering, computing & technology: Multidisciplinary, general & others
Author, co-author :
Hall, Gabriella Nilsson;  Prometheus, Div. of Skeletal Tissue Engineering, KU Leuven, Belgium, Skeletal
Chandrakar, Amit;  Department of Complex Tissue Regeneration, Maastricht University, The
Pastore, Angela;  Prometheus, Div. of Skeletal Tissue Engineering, KU Leuven, Belgium, Skeletal
Ioannidis, Konstantinos;  Prometheus, Div. of Skeletal Tissue Engineering, KU Leuven, Belgium, Skeletal
Moisley, Katrina;  The Electrospinning Company Ltd, England.
Cirstea, Matei;  The Electrospinning Company Ltd, England.
Geris, Liesbet  ;  Université de Liège - ULiège > Département d'aérospatiale et mécanique > Génie biomécanique ; Prometheus, Div. of Skeletal Tissue Engineering, KU Leuven, Belgium, Biomechanics
Moroni, Lorenzo;  Department of Complex Tissue Regeneration, Maastricht University, The
Luyten, Frank P;  Prometheus, Div. of Skeletal Tissue Engineering, KU Leuven, Belgium.
Wieringa, Paul;  Department of Complex Tissue Regeneration, Maastricht University, The
Papantoniou, Ioannis;  Prometheus, Div. of Skeletal Tissue Engineering, KU Leuven, Belgium, Skeletal
Language :
English
Title :
Engineering bone-forming biohybrid sheets through the integration of melt electrowritten membranes and cartilaginous microspheroids.
Publication date :
22 October 2022
Journal title :
Acta Biomaterialia
ISSN :
1742-7061
eISSN :
1878-7568
Publisher :
Elsevier, Nl
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright © 2022. Published by Elsevier Ltd.
Available on ORBi :
since 05 July 2023

Statistics


Number of views
45 (1 by ULiège)
Number of downloads
35 (0 by ULiège)

Scopus citations®
 
2
Scopus citations®
without self-citations
2
OpenCitations
 
0

Bibliography


Similar publications



Contact ORBi