In vitro virucidal activity of nebulized citrate-complexed silver nanoparticles against equine herpesvirus-1 and murine norovirus

Frippiat, Thibault; Dams, Lorène; Wielick, Constance; Delguste, Catherine; Ludwig-Begall, Louisa; Art, Tatiana; Thiry, Etienne

doi:10.1016/j.virol.2023.06.003

Article (Scientific journals)

In vitro virucidal activity of nebulized citrate-complexed silver nanoparticles against equine herpesvirus-1 and murine norovirus

Frippiat, Thibault; Dams, Lorène; Wielick, Constance et al.

2023 • In Virology

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/304813

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10.1016/j.virol.2023.06.003

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Keywords :

Virology

Abstract :

[en] Viruses can be involved in respiratory disorders in horses, with limited therapeutic options. Citrate-complexed silver nanoparticles (C–AgNP) have shown bactericidal properties after in vitro nebulization. The aim of the present study was to assess the virucidal activity of C–AgNP after in vitro instillation or nebulization on equine herpesvirus-1 (EHV-1) and murine norovirus (MNV), the latter used as surrogate for small non-enveloped viruses. Both viruses were instilled or nebulized with C–AgNP of increasing concentrations, and titres were determined via TCID50 method. We demonstrated efficient inactivation of enveloped EHV-1 following instillation and nebulization of C–AgNP (infectivity losses of ≥ three orders of magnitude). While tenacious MNV was inactivated via 2000 ppm C–AgNP instillation, nebulized C–AgNP did not lead to reduction in MNV titres. Nebulization of C–AgNP may represent a novel virucidal therapeutic approach in horses. Further investigations are needed to assess its safety and effective concentrations for in vivo use.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Frippiat, Thibault ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH)

Dams, Lorène ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI)

Wielick, Constance ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire

Delguste, Catherine ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée

Ludwig-Begall, Louisa ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire

Art, Tatiana ; Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > Physiologie

Thiry, Etienne ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI)

Language :

English

Title :

In vitro virucidal activity of nebulized citrate-complexed silver nanoparticles against equine herpesvirus-1 and murine norovirus

Publication date :

13 June 2023

Journal title :

Virology

ISSN :

0042-6822

eISSN :

1096-0341

Publisher :

Elsevier BV

Peer reviewed :

Peer Reviewed verified by ORBi

Data Set :

https://doi.org/10.1016/j.virol.2023.06.003

Available on ORBi :

since 03 July 2023

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