Absorption; Food additive; Intestine; Microbiota; Nutrition; Phospholipid; Medicine (miscellaneous); Food Science; Nutrition and Dietetics
Abstract :
[en] Synthetic emulsifiers promote metabolic syndrome and considerably alter gut microbiota. Data is lacking regarding natural emulsifiers like plant lecithins, a polar lipid-rich source of 18:3n-3 PUFA (ALA). For 13 weeks, male Swiss mice were fed ALA-replete semi-synthetic high-fat diet (HFD) including lecithin from rapeseed (RL) or soy, vs 2 HFD-controls devoid of lecithin (ALA-replete; low-ALA), vs Chow. Lecithins did not enhance HFD-induced adiposity nor increased inflammation, did not alter gut barrier markers and caecal bile acids, and contributed to n-3 PUFA status. Lecithins improved gut microbiota diversity. RL (10% in fat) even restored α-diversity similar to Chow, increased Lachnospiraceae NK4A136, Lactobacillus and Ruminococcaceae UCG-014 groups, and decreased Blautia genus bacteria. The abundance of most beneficial lecithin-enhanced bacteria was positively correlated to the amount of faecal polar lipid-bound ALA. These findings show that lecithins can beneficially affect the gut microbiota in association with changes in lipid residues in the distal gut.
Disciplines :
Food science
Author, co-author :
Robert, Chloé; Univ-Lyon, CarMeN laboratory, INSERM, INRAE, Pierre Bénite, France ; ITERG, Equipe Nutrition, Santé et Biochimie des Lipides, Canéjan, France
Penhoat, Armelle; Univ-Lyon, CarMeN laboratory, INSERM, INRAE, Pierre Bénite, France
Couëdelo, Leslie; ITERG, Equipe Nutrition, Santé et Biochimie des Lipides, Canéjan, France
Monnoye, Magali; Micalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, France
Rainteau, Dominique; Sorbonne Université, INSERM, Centre de Recherche Saint-Antoine, CRSA, AP-HP, Saint Antoine Hospital, Clinical Metabolomics Department, France
Meugnier, Emmanuelle; Univ-Lyon, CarMeN laboratory, INSERM, INRAE, Pierre Bénite, France
Bary, Sofia; ITERG, Equipe Nutrition, Santé et Biochimie des Lipides, Canéjan, France
Abrous, Hélène; ITERG, Equipe Nutrition, Santé et Biochimie des Lipides, Canéjan, France
Loizon, Emmanuelle; Univ-Lyon, CarMeN laboratory, INSERM, INRAE, Pierre Bénite, France
Krasniqi, Pranvera; Sorbonne Université, INSERM, Centre de Recherche Saint-Antoine, CRSA, AP-HP, Saint Antoine Hospital, Clinical Metabolomics Department, France
Chanon, Stéphanie; Univ-Lyon, CarMeN laboratory, INSERM, INRAE, Pierre Bénite, France
Vieille-Marchiset, Aurélie; Univ-Lyon, CarMeN laboratory, INSERM, INRAE, Pierre Bénite, France
Caillet, François; Univ-Lyon, CarMeN laboratory, INSERM, INRAE, Pierre Bénite, France
Danthine, Sabine ; Université de Liège - ULiège > TERRA Research Centre > Technologie Alimentaire (TA)
Vidal, Hubert; Univ-Lyon, CarMeN laboratory, INSERM, INRAE, Pierre Bénite, France
Guillot, Nicolas; Univ-Lyon, CarMeN laboratory, INSERM, INRAE, Pierre Bénite, France ; LIBM, INSA-Lyon, France
Gérard, Philippe; Micalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, France
Vaysse, Carole; ITERG, Equipe Nutrition, Santé et Biochimie des Lipides, Canéjan, France
Michalski, Marie-Caroline; Univ-Lyon, CarMeN laboratory, INSERM, INRAE, Pierre Bénite, France
The authors would like to thank Béatrice Morio, Frédéric Capel and Mélanie Le Barz for helpful advice on experimental procedures and useful discussion of the results. The authors thank Alexandre Debain for this help during the animal study. We also thank Didier Majou (ACTIA) for useful discussion. CR thanks ANRT (Association Nationale de la Recherche et de la Technologie) for PhD funding (CIFRE 2017/12/2). CarMeN and ITERG are members of UMT ACTIA BALI. CR is grateful to GERLI and Société Francophone de Nutrition for the GERLI-SFN Thesis Prize 2022. We are grateful to the @BRIDGe sequencing facility (GABI, INRAE, AgroParisTech, Paris-Saclay University) and to the INRAE MIGALE bioinformatics facility (MIGALE, INRAE, 2020. Migale bioinformatics Facility, doi: 10.15454/1.5572390655343293E12) for providing help and storage resources. SD acknowledges the support of Wallonie-Bruxelles International and Fonds de la Recherche scientifique (FNRS). MCM acknowledges Partenariat Hubert Curien PHC TOURNESOL project N°46226VD for funding. Part of the artwork is derived from Freepik, Pixabay and Servier Medical Art.The authors would like to thank Béatrice Morio, Frédéric Capel and Mélanie Le Barz for helpful advice on experimental procedures and useful discussion of the results. The authors thank Alexandre Debain for this help during the animal study. We also thank Didier Majou (ACTIA) for useful discussion. CR thanks ANRT (Association Nationale de la Recherche et de la Technologie) for PhD funding (CIFRE 2017/12/2). CarMeN and ITERG are members of UMT ACTIA BALI. CR is grateful to GERLI and Société Francophone de Nutrition for the GERLI-SFN Thesis Prize 2022. We are grateful to the @BRIDGe sequencing facility (GABI, INRAE, AgroParisTech, Paris-Saclay University) and to the INRAE MIGALE bioinformatics facility (MIGALE, INRAE, 2020. Migale bioinformatics Facility, doi: 10.15454/1.5572390655343293E12) for providing help and storage resources. SD acknowledges the support of Wallonie-Bruxelles International and Fonds de la Recherche scientifique (FNRS). MCM acknowledges Partenariat Hubert Curien PHC TOURNESOL project N°46226VD for funding. Part of the artwork is derived from Freepik, Pixabay and Servier Medical Art.
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