A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOT ® Technology for Assessing Total Anti-Oxidative Capacity
_A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility - Biomedicines - Pincemail - 04-2023.pdf
[en] Background: Oxidative stress (OS) could cause various COVID-19 complications. Recently, we have developed the Pouvoir AntiOxydant Total (PAOT®) technology for reflecting the total antioxidant capacity (TAC) of biological samples. We aimed to investigate systemic oxidative stress status (OSS) and to evaluate the utility of PAOT®for assessing TAC during the recovery phase in critical COVID-19 patients in a rehabilitation facility. Materials and Methods: In a total of 12 critical COVID-19 patients in rehabilitation, 19 plasma OSS biomarkers were measured: antioxidants, TAC, trace elements, oxidative damage to lipids, and inflammatory biomarkers. TAC level was measured in plasma, saliva, skin, and urine, using PAOT and expressed as PAOT-Plasma, -Saliva, -Skin, and -Urine scores, respectively. Plasma OSS biomarker levels were compared with levels from previous studies on hospitalized COVID-19 patients and with the reference population. Correlations between four PAOT scores and plasma OSS biomarker levels were analyzed. Results: During the recovery phase, plasma levels in antioxidants ( -tocopherol, -carotene, total glutathione, vitamin C and thiol proteins) were significantly lower than reference intervals, whereas total hydroperoxides and myeloperoxidase (a marker of inflammation) were significantly higher. Copper negatively correlated with total hydroperoxides (r = 0.95, p = 0.001). A similar, deeply modified OSS was already observed in COVID-19 patients hospitalized in an intensive care unit. TAC evaluated in saliva, urine, and skin correlated negatively with copper and with plasma total hydroperoxides. To conclude, the systemic OSS, determined using a large number of biomarkers, was always significantly increased in cured COVID-19 patients during their recovery phase. The less costly evaluation of TAC using an electrochemical method could potentially represent a good alternative to the individual analysis of biomarkers linked to pro-oxidants.
Disciplines :
Laboratory medicine & medical technology Orthopedics, rehabilitation & sports medicine
Author, co-author :
Pincemail, Joël ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Rousseau, Anne-Françoise ; Centre Hospitalier Universitaire de Liège - CHU > > Service des soins intensifs
Kaux, Jean-François ; Centre Hospitalier Universitaire de Liège - CHU > > Service de médecine de l'appareil locomoteur
CHERAMY-BIEN, Jean-Paul ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chirurgie cardio-vasculaire et thoracique
Bruyere, Christine ; Centre Hospitalier Universitaire de Liège - CHU > > Service de médecine de l'appareil locomoteur
PRICK, Jeanine ; Centre Hospitalier Universitaire de Liège - CHU > > Unité de médecine B
Stern, David ; Université de Liège - ULiège > GIGA > GIGA Platform Genomics
Kaci, Mouna-Messaouda; Research Department, Institut Européen des Antioxydants (IEA), Oxystress Technologies PAOTScan, Vandoeuvre-lès-Nancy, France
Albert, Adelin ; Université de Liège - ULiège > Département des sciences de la santé publique
Eubelen, Céline ; Centre Hospitalier Universitaire de Liège - CHU > > Service de médecine de l'appareil locomoteur
Le Goff, Caroline ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Misset, Benoît ; Centre Hospitalier Universitaire de Liège - CHU > > Service des soins intensifs
Cavalier, Etienne ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chimie clinique
Charlier, Corinne ; Centre Hospitalier Universitaire de Liège - CHU > > Service de toxicologie clinique, médicolégale, environnementale et en entreprise
Meziane, Smail; Research Department, Institut Européen des Antioxydants (IEA), Oxystress Technologies PAOTScan, Vandoeuvre-lès-Nancy, France
A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOT ® Technology for Assessing Total Anti-Oxidative Capacity
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