Article (Scientific journals)
Atmospheric-Pressure Plasma Deposited Epoxy-Rich Thin Films as Platforms for Biomolecule Immobilization-Application for Anti-Biofouling and Xenobiotic-Degrading Surfaces
Camporeale, Giuseppe; Moreno-Couranjou, Maryline; Bonot, Sébastien et al.
2015In Plasma Processes and Polymers, 12 (11), p. 1208-1219
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Keywords :
atmospheric-pressure dielectric barrier discharges; bioactive surfaces; immobilization of biomolecules; plasma polymerization; plasma-enhanced chemical vapor deposition (PECVD); Polymers and Plastics; Condensed Matter Physics
Abstract :
[en] In this work, an atmospheric-pressure dielectric barrier discharge process is exploited for the fast deposition of adherent epoxy-rich layers acting as a versatile platform for the efficient one-step biomolecule immobilization in mild aqueous conditions. Particular attention is given to the influence of the plasma process parameters on the chemical and morphological properties of the deposited layers and on their subsequent exploitation for chemical interfacial reactions. As a proof-of-concept, two enzymes with drastically different biological properties, namely dispersin B and a laccase, are immobilized onto functionalized metallic surfaces. The pH of the enzyme solution appears as a key parameter to control the amount of immobilized enzyme on the plasma functionalized surfaces, thus leading to bioactive surfaces with improved stability and activity.
Disciplines :
Biotechnology
Author, co-author :
Camporeale, Giuseppe;  Universita degli studi di Bari > Dipartimento di Chimica
Moreno-Couranjou, Maryline;  LIST - Luxembourg Institute of Science and Technology [LU]
Bonot, Sébastien;  LIST - Luxembourg Institute of Science and Technology [LU]
Mauchauffé, Rodolphe;  LIST - Luxembourg Institute of Science and Technology [LU]
Boscher, Nicolas;  LIST - Luxembourg Institute of Science and Technology [LU]
Bebrone, Carine ;  Université de Liège - ULiège > GIGA > GIGA Platforms
Van de Weerdt, Cécile ;  Université de Liège - ULiège > GIGA > GIGA Cardiovascular Sciences - Molecular Biomimetic and Protein Engineering Laboratory
Cauchie, Henry-Michel;  Unilu - Université du Luxembourg [LU] > Luxembourg Institute of Science and Technology (LIST)
Favia, Pietro;  Università Degli Studi di Bari aldo Moro
Choquet, Patrick;  Unilu - Université du Luxembourg [LU] > Luxembourg Institute of Science and Technology (LIST)
Language :
English
Title :
Atmospheric-Pressure Plasma Deposited Epoxy-Rich Thin Films as Platforms for Biomolecule Immobilization-Application for Anti-Biofouling and Xenobiotic-Degrading Surfaces
Publication date :
15 May 2015
Journal title :
Plasma Processes and Polymers
ISSN :
1612-8850
eISSN :
1612-8869
Publisher :
Wiley
Volume :
12
Issue :
11
Pages :
1208-1219
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FNR - Fonds National de la Recherche
SPW DGO6 - Service Public de Wallonie. Economie, Emploi, Recherche
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since 30 April 2023

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