The three-dimensional structure of VIM-31a metallo-blactamase from Enterobacter cloacae in its native and oxidized form

active site; antibiotic resistance; crystallographic structures; Enterobacter cloacae; metallo-b-lactamase Abbreviations DLS, dynamic light scattering; MBL, metallo-b-lactamase; PDB, Protein Data Bank; TCEP, Tris(2-carboxyethyl)phosphine; Cell Biology; Molecular Biology; Biochemistry

Abstract :

[en] His and His252Arg substitutions. Located close to the active site, the Tyr224His substitution is also present in VIM-1, VIM-4, VIM-7 and VIM-12. The VIM-31 variant was reported in 2012 from Enterobacter cloacae and kinetically characterized. It exhibits globally lower catalytic efficiencies than VIM-2. In the present study, we report the three-dimensional structures of VIM-31 in its native (reduced) and oxidized forms. The so-called 'flapping-loop' (loop 1) and loop 3 of VIM-31 were not positioned as in VIM-2 but instead were closer to the active site as in VIM-4, resulting in a narrower active site in VIM-31. Also, the presence of His224 in VIM-31 disrupts hydrogen-bonding networks close to the active site. Moreover, a third zinc-binding site, which also exists in VIM-2 structures, could be identified as a structural explanation for the decreased activity of VIM-MBLs at high zinc concentrations.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Kupper, Michaël B.; Institute of Molecular Biotechnology, RWTH-Aachen University, Germany

Herzog, Konrad; Institute of Molecular Biotechnology, RWTH-Aachen University, Germany

Bennink, Sandra; Institute of Molecular Biotechnology, RWTH-Aachen University, Germany

Schlömer, Philipp; Institute of Molecular Biotechnology, RWTH-Aachen University, Germany

Bogaerts, Pierre; Laboratory of Bacteriology, CHU Mont-Godinne-Dinant, Universit e Catholique de Louvain, Yvoir, Belgium

Glupczynski, Youri; Laboratory of Bacteriology, CHU Mont-Godinne-Dinant, Universit e Catholique de Louvain, Yvoir, Belgium

Fischer, Rainer; Institute of Molecular Biotechnology, RWTH-Aachen University, Germany

Bebrone, Carine ^✱; Université de Liège - ULiège > GIGA > GIGA Platforms

Hoffmann, Kurt ^✱; Institute of Molecular Biotechnology, RWTH-Aachen University, Germany

^✱ These authors have contributed equally to this work.

Language :

English

Title :

The three-dimensional structure of VIM-31a metallo-blactamase from Enterobacter cloacae in its native and oxidized form

Publication date :

13 April 2015

Journal title :

FEBS Journal

ISSN :

1742-464X

eISSN :

1742-4658

Publisher :

Wiley

Volume :

282

Issue :

Pages :

2352-2360

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Ffebs.13283

Available on ORBi :

since 30 March 2023

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