Animals; In Vitro Techniques; Jaw/radiation effects; Mouth Mucosa/radiation effects; Swine; Body Temperature; Hot Temperature; Lasers, Solid-State; Low level laser therapy; Nd: YAG laser; Thermal camera; Thermal increase; Thermocouple; Surgery; Otorhinolaryngology; Dentistry (all); General Dentistry
Abstract :
[en] [en] OBJECTIVE: Literature reports bactericidal and biostimulant effects for Nd:YAG laser procedures on bone and oral mucosa but the possible overheating can cause damage to anatomical structures. The aim of the study was to evaluate the is the evaluation of thermal increase in different levels of oral tissues: mucosa, periosteum and bone during defocused application of Nd:YAG laser at different parameters.
STUDY DESIGN: Superficial thermal evaluation was performed in pig jaws with a thermal camera device; deep thermal evaluation was realized by 4 thermocouples placed at a subperiosteal level and at 1,2 and 4 mm depth in the jaw bone. Laser applications of 1 minute were performed 5 times (with a pause of 1 minute) on a surface of 4 cm² with a Nd:YAG laser (MSP mode, 320 micrometer fiber, defocused mode) with different parameters. Temperatures were recorded before and after laser applications and after each pause in order to evaluate also the thermal relaxation of tissues.
RESULTS: At submucosal level, mean thermal increase was between 1.1°C and 13.2°C, at 1 mm depth between 1.1°C and 8.5°C, at 2 mm depth between 1.1°C and 6.8°C, at 4 mm depth between 1.0°C and 5.3°C. Temperature decrease during the rest time period was variable between 0°C and 2.5°C.
CONCLUSIONS: Temperatures reached during clinical procedures with parameters reported in the literature in biostimulation protocols (1.25-2 Watts) for the five minutes of application are not dangerous for biological structures. The decrease in temperature during the rest time period is less considerable in the bone in comparison to oral mucosa.
Disciplines :
Dentistry & oral medicine
Author, co-author :
Vescovi, Paolo; Unit of Oral Medicine, Oral Pathology and Laser-Assisted Oral Surgery, Department of ENT-Dental Ophtalmological and Cervico-Facial Sciences, University of Parma, Italy
Merigo, Elisabetta; Faculty of Medicine, University of Liège, Liège, Belgium
Fornaini, Carlo; School in Dental sciences, Faculty of Medicine, University of Liège, Liège, Belgium
Rocca, Jean-Paul; TELEO Laboratory, Faculty of Dentistry, University of Nice Sophia Antipolis, Nice, France
Namour, Samir ; Université de Liège - ULiège > Département des sciences dentaires
Language :
English
Title :
Thermal increase in the oral mucosa and in the jawbone during Nd:YAG laser applications. Ex vivo study.
Angiero F, Benedicenti S, Romanos GE, Crippa R. Treatment of hemangioma of the head and neck with diode laser and forced dehydration with induced photocoagulation. Photomed Laser Surg. 2008;26:113-118.
Pirnat S, Lukac M, Ihan A. Study of the direct bactericidal effect of Nd:YAG and diode laser parameters used in endodontics on pigmented and nonpigmented bacteria. Lasers Med Sci. 2011;26:755-761.
Chellini F, Sassoli C, Nosi D, Deledda C, Tonelli P, Zecchi-Or-landini S, et al. Low pulse energy Nd:YAG laser irradiation exerts a biostimulative effect on different cells of the oral microenvironment: "an in vitro study". Lasers Surg Med. 2010;42:527-539.
Giannelli M, Bani D, Tani A, Pini A, Margheri M, Zecchi-Or-landini S, et al. In vitro evaluation of the effects of low-intensity Nd:YAG laser irradiation on the inflammatory reaction elicited by bacterial lipopolysaccharide adherent to titanium dental implants. J Periodontol. 2009;80:977-984.
Khadra M, Kazem N, Haanaes HR, Ellingsen JE, Lyngstadaas SP. Enhancement of bone formation in rat calvarial bone defects using low-level laser therapy. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004;97:693-700.
Karu T. Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol B. 1999;49:1-17.
Bashardoust TS, Macdermid JC, Houghton P, Grewal R. Effects of low power laser irradiation on bone healing in animals: a meta-analysis. J Orthop Surg Res. 2010;5:1.
David R, Nissan M, Cohen I, Soudry M. Effect of low-power He-Ne laser on fracture healing in rats. Lasers Surg Med. 1996;19:458-464.
Luger EJ, Rochkind S, Wollman Y, Kogan G, Dekel S. Effect of low-power laser irradiation on the mechanical properties of bone fracture healing in rats. Lasers Surg Med. 1998;22:97-102.
Grönqvist A, Wiström J, Axner O, Monsen TJ. Bactericidal effect of pulsed 1,064 nm Nd:YAG laser light on Staphylococcus epider-midis is of photothermal origin: an in vitro study. Lasers Surg Med. 2000;27:336-340.
Bahcall J, Howard P, Miserendino L, Walia H. Preliminary investigation of the histological effects of laser endodontic treatment on the periradicular tissues in dogs. J Endod. 1992;18:47-51.
Ramsköld LO, Fong CD, Strömberg T. Thermal effects and antibacterial properties of energy levels required to sterilize stained root canals with an Nd: YAG laser. J Endod. 1997;23:96-100.
Nammour S, Kowaly K, Powell GL, Van Reck J, Rocca J P. External temperature during KTP-Nd: YAG laser irradiation in root canals: an in vitro study. Lasers Med Sci. 2004;19:27-32.
Zach L, Cohen G. Pulp response to externally applied heat. Oral Surg Oral Med Oral Pathol. 1965;19:515-530.
el Yazami H, Zeinoun T, Bou Saba S, Lamard L, Peremans A, Limme M, et al. Pulp temperature increase during photo-activated disinfection (PAD) of periodontal pockets: an in vitro study. Lasers Med Sci. 2010;25:655-659.
Nammour S, Zeinoun T, Bogaerts I, Lamy M, Geerts SO, Bou Saba S, et al. Evaluation of dental pulp temperature rise during photo-activated decontamination (PAD) of caries: an in vitro study. Lasers Med Sci. 2010;25:651-654.
Foth H, Färber S, Gauer A, Wagner R. Thermal damage threshold at 633 nm of tympanic membrane of pig. Hear Res. 2000;142:71-78.
Ricci T, Mazzoni M. Experimental investigation of temperature gradients in the inner ear following argon laser exposure. J Laryngol Otol. 1985;99:359-362.
Gómez-Santos L, Arnabat-Domínguez J, Sierra-Rebolledo A, Gay-Escoda C. Thermal increment due to ErCr:YSGG and CO2 laser irradiation of different implant surfaces. A pilot study. Med Oral Patol Oral Cir Bucal. 2010;15:e782-787.
Vescovi P, Merigo E, Manfredi M, Meleti M, Fornaini C, Bonani-ni M, et al. Nd:YAG laser biostimulation in the treatment of bisphos-phonate-associated osteonecrosis of the jaw: clinical experience in 28 cases. Photomed Laser Surg. 2008;26:37-46.
Vescovi P, Corcione L, Meleti M, Merigo E, Fornaini C, Manfredi M, et al. Nd:YAG laser versus traditional scalpel. A preliminary histological analysis of specimens from the human oral mucosa. Lasers Med Sci. 2010;25:685-691.
Sedlin ED, Hirsch C. Factors affecting the determination of the physical properties of femoral cortical bone. Acta Orthop Scand. 1966;37:29-48.
Fornaini C, Bertrand C, Rocca JP, Mahler P, Bonanini M, Vescovi P, et al.Intra-oral laser welding: an in vitro evaluation of thermal increase. Laser Med Sci. 2010;25:473-477.
Fornaini C, Vescovi P, Merigo E, Rocca JP, Mahler P, Bertrand C, et al. Intraoral metal laser welding: a case report. Lasers Med Sci. 2010;25:303-307.
Martins GR, Cavalcanti BN, Rode SM. Increases in intrapulpal temperature during polymerization of composite resin. J Prosthet Dent. 2006;96:328-331.
Sulieman M, Rees JS, Addy M. Surface and pulp chamber temperature rises during tooth bleaching using a diode laser: a study in vitro. Br Dent J. 2006;200:631-634.
Mehl A, Kremers L, Salzmann K, Hickel R. 3D volume-ablation rate and thermal side effects with the Er:YAG and Nd:YAG laser. Dent Mater. 1997;13:246-251.
Augustin G, Davila S, Mihoci K, Udiljak T, Vedrina DS, Antabak A. Thermal osteonecrosis and bone drilling parameters revisited. Arch Orthop Trauma Surg. 2008;128:71-77.
Raiss P, Pape G, Jäger S, Loew M, Bitsch R, Rickert M. In vitro measurement of temperature changes during implantation of cemented glenoid components. Acta Orthop. 2010;81:211-215.
Eriksson AR, Albrektsson T. Temperature threshold levels for heat-induced bone tissue injury: a vital-microscopic study in the rabbit. J Prosthet Dent. 1983;50:101-107.
