Article (Scientific journals)
Virus-induced interference as a means for accelerating fitness-based selection of cyprinid herpesvirus 3 single-nucleotide variants in vitro and in vivo.
Gao, Yuan; Sridhar, Arun; Bernard, Noah et al.
2023In Virus Evolution, 9 (1), p. 003
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Keywords :
alloherpesvirus; fitness-based selection; herpesvirus; purifying selection; virus evolution; Virology; Microbiology
Abstract :
[en] Cyprinid herpesvirus 3 (CyHV-3) is the archetype of fish alloherpesviruses and is advantageous to research because, unlike many herpesviruses, it can be studied in the laboratory by infection of the natural host (common and koi carp). Previous studies have reported a negative correlation among CyHV-3 strains between viral growth in vitro (in cell culture) and virulence in vivo (in fish). This suggests the existence of genovariants conferring enhanced fitness in vitro but reduced fitness in vivo and vice versa. Here, we identified the syncytial plaque formation in vitro as a common trait of CyHV-3 strains adapted to cell culture. A comparison of the sequences of virion transmembrane protein genes in CyHV-3 strains, and the use of various recombinant viruses, demonstrated that this trait is linked to a single-nucleotide polymorphism (SNP) in the open reading frame (ORF) 131 coding sequence (C225791T mutation) that results in codon 183 encoding either an alanine (183A) or a threonine (183T) residue. In experiments involving infections with recombinant viruses differing only by this SNP, the 183A genovariant associated with syncytial plaque formation was the more fit in vitro but the less fit in vivo. In experiments involving coinfection with both viruses, the more fit genovariant contributed to the purifying selection of the less fit genovariant by outcompeting it. In addition, this process appeared to be accelerated by viral stimulation of interference at a cellular level and stimulation of resistance to superinfection at a host level. Collectively, this study illustrates how the fundamental biological properties of some viruses and their hosts may have a profound impact on the degree of diversity that arises within viral populations.
Disciplines :
Genetics & genetic processes
Author, co-author :
Gao, Yuan ;  Université de Liège - ULiège > Université de Liège - ULiège
Sridhar, Arun ;  Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Bernard, Noah ;  Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
He, Bo ;  Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH)
Zhang, Haiyan  ;  Université de Liège - ULiège > GIGA - MASSGPFS
Pirotte, Sébastien  ;  Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Desmecht, Salomé  ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI)
Vancsok, Catherine ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Immunologie vétérinaire
Boutier, Maxime ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Immunologie vétérinaire
Suárez, Nicolás M ;  MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK
Davison, Andrew J;  MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK
Donohoe, Owen  ;  Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire ; Bioscience Research Institute, Technological University of the Shannon, Midlands Midwest, Athlone, Co. Westmeath N37HD68, Ireland
Vanderplasschen, Alain ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Immunologie vétérinaire
More authors (3 more) Less
Language :
English
Title :
Virus-induced interference as a means for accelerating fitness-based selection of cyprinid herpesvirus 3 single-nucleotide variants in vitro and in vivo.
Publication date :
2023
Journal title :
Virus Evolution
eISSN :
2057-1577
Publisher :
Oxford University Press (OUP), England
Volume :
9
Issue :
1
Pages :
vead003
Peer reviewed :
Peer reviewed
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
ULiège - Université de Liège [BE]
MRC - Medical Research Council [GB]
WELBIO - Walloon Excellence in Life Sciences and Biotechnology [BE]
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