Article (Scientific journals)
Prospective flow cytometry analysis of leucocyte subsets in critically ill patients who develop sepsis: a pilot study.
Layios, Nathalie; Gosset, Christian; Maes, Nathalie et al.
2023In Infection
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Keywords :
Flow cytometry; HLA-DR; Injury; L-selectin; Monocytes; Sepsis; Infectious Diseases; critical care
Abstract :
[en] ("[en] PURPOSE: Sepsis in critically ill patients with injury bears a high morbidity and mortality. Extensive phenotypic monitoring of leucocyte subsets in critically ill patients at ICU admission and during sepsis development is still scarce. The main objective of this study was to identify early changes in leukocyte phenotype which would correlate with later development of sepsis. METHODS: Patients who were admitted in a tertiary ICU for organ support after severe injury (elective cardiac surgery, trauma, necessity of prolonged ventilation or stroke) were sampled on admission (T1) and 48-72 h later (T2) for phenotyping of leukocyte subsets by flow cytometry and cytokines measurements. Those who developed secondary sepsis or septic shock were sampled again on the day of sepsis diagnosis (Tx). RESULTS: Ninety-nine patients were included in the final analysis. Nineteen (19.2%) patients developed secondary sepsis or septic shock. They presented significantly higher absolute monocyte counts and CRP at T1 compared to non-septic patients (1030/µl versus 550/µl, p = 0.013 and 5.1 mg/ml versus 2.5 mg/ml, p = 0.046, respectively). They also presented elevated levels of monocytes with low expression of L-selectin (CD62Lneg monocytes) (OR[95%CI] 4.5 (1.4-14.5), p = 0.01) and higher SOFA score (p < 0.0001) at T1 and low mHLA-DR at T2 (OR[95%CI] 0.003 (0.00-0.17), p = 0.049). Stepwise logistic regression analysis showed that both monocyte markers and high SOFA score (> 8) were independently associated with nosocomial sepsis occurrence. No other leucocyte count or surface marker nor any cytokine measurement correlated with sepsis occurrence. CONCLUSION: Monocyte counts and change of phenotype are associated with secondary sepsis occurrence in critically ill patients with injury.","[en] ","")
Research center :
GIGA Cardiovascular Sciences-Cardiology - ULiège
Disciplines :
Anesthesia & intensive care
Author, co-author :
Layios, Nathalie  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service des soins intensifs
Gosset, Christian ;  Centre Hospitalier Universitaire de Liège - CHU > > Service d'hématologie biologique et immuno-hématologie
Maes, Nathalie ;  Centre Hospitalier Universitaire de Liège - CHU > > Service des informations médico économiques (SIME)
Delierneux, Céline ;  Université de Liège - ULiège > GIGA > GIGA Cardiovascular Sciences - Cardiology
Hego, Alexandre ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques
Huart, Justine  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de néphrologie
LECUT, Christelle ;  Centre Hospitalier Universitaire de Liège - CHU > > Service d'hématologie biologique et immuno-hématologie
DAMAS, Pierre ;  Centre Hospitalier Universitaire de Liège - CHU > > Service des soins intensifs
Oury, Cécile  ;  Université de Liège - ULiège > GIGA > GIGA Cardiovascular Sciences - Cardiology
Gothot, André ;  Centre Hospitalier Universitaire de Liège - CHU > > Service d'hématologie biologique et immuno-hématologie
Language :
English
Title :
Prospective flow cytometry analysis of leucocyte subsets in critically ill patients who develop sepsis: a pilot study.
Publication date :
25 January 2023
Journal title :
Infection
ISSN :
0300-8126
eISSN :
1439-0973
Publisher :
Springer Science and Business Media LLC, Germany
Peer reviewed :
Peer Reviewed verified by ORBi
Data Set :
Available on ORBi :
since 26 February 2023

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