Differential Kendrick's Plots as an Innovative Tool for Lipidomics in Complex Samples: Comparison of Liquid Chromatography and Infusion-Based Methods to Sample Differential Study.
Lipids; Lipidomics; Biological samples; Complex samples; Direct infusions; Kendrick plots; Liquid chromatography - mass spectrometries; Non-targeted; Structural diversity; Visualization tools
Abstract :
[en] Lipidomics has developed rapidly over the past decade. Nontargeted lipidomics from biological samples remains a challenge due to the high structural diversity, the concentration range of lipids, and the complexity of biological samples. We introduce here the use of differential Kendrick's plots as a rapid visualization tool for a qualitative nontargeted analysis of lipids categories and classes from data generated by either liquid chromatography-mass spectrometry (LC-MS) or direct infusion (nESI-MS). Each lipid class is easily identified by comparison with the theoretical Kendrick plot pattern constructed from exact mass measurements and by using MSKendrickFilter, an in-house Python software. The lipids are identified with the LIPID MAPS database. In addition, in LC-MS, the software based on the Kendrick plots returns the retention time from all the lipids belonging to the same series. Lipid extracts from a yeast (Saccharomyces cerevisiae) are used as a model. An on/off case comparing Kendrick plots from two cell lines (prostate cancer cell lines treated or not with a DGAT2 inhibition) clearly shows the effect of the inhibition. Our study demonstrates the good performance of direct infusion as a fast qualitative screening method as well as for the analysis of chromatograms. A fast screening semiquantitative approach is also possible, while the targeted mode remains the golden standard for precise quantitative analysis.
Disciplines :
Chemistry
Author, co-author :
Hustin, Justine ; Université de Liège - ULiège > Molecular Systems (MolSys)
Kune, Christopher ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Far, Johann ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Eppe, Gauthier ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Debois, Delphine ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) ; ZenTech S.A., Avenue du Pré Aily 10, 4031Liège, Belgique
Quinton, Loïc ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie biologique
De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences)
Language :
English
Title :
Differential Kendrick's Plots as an Innovative Tool for Lipidomics in Complex Samples: Comparison of Liquid Chromatography and Infusion-Based Methods to Sample Differential Study.
Publication date :
07 December 2022
Journal title :
Journal of the American Society for Mass Spectrometry
We thank the Interreg EMR project EURLIPIDS (R-8598) for financial support. We acknowledge Lipometrix and the KU Leuven lipidomics core who cultured the cells and performed the lipid extraction on those. Thank you to the FEDER BIOMED HUB Technology Support (number 2.2.1/996) and the EU_FT-ICR_MS network (under Grant Agreement No.73107) for the funding of FTRMS on the SolariX XR 9.4T FT-ICR. Finally, we acknowledge Johnson & Johnson (Janssen) for the donation of the LTQ FT Ultra 7T instrument; H2020 Research Infrastructures
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