[en] The mammalian pancreas is a branched organ that does not exhibit stereotypic branching patterns, similarly to most other glands. Inside branches, it contains a network of ducts that undergo a transition from unconnected microlumen to a mesh of interconnected ducts and finally to a treelike structure. This ductal remodeling is poorly understood, both on a microscopic and macroscopic level. In this article, we quantify the network properties at different developmental stages. We find that the pancreatic network exhibits stereotypic traits at each stage and that the network properties change with time toward the most economical and optimized delivery of exocrine products into the duodenum. Using in silico modeling, we show how steps of pancreatic network development can be deconstructed into two simple rules likely to be conserved for many other glands. The early stage of the network is explained by noisy, redundant duct connection as new microlumens form. The later transition is attributed to pruning of the network based on the flux of fluid running through the pancreatic network into the duodenum.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Dahl-Jensen, Svend Bertel; DanStem, University of Copenhagen, Copenhagen, Denmark ; Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark
Yennek, Siham; DanStem, University of Copenhagen, Copenhagen, Denmark
Flasse, Lydie ; Université de Liège - ULiège > GIGA > GIGA Stem Cells - Zebrafish Development and Disease Model ; DanStem, University of Copenhagen, Copenhagen, Denmark
Larsen, Hjalte List; DanStem, University of Copenhagen, Copenhagen, Denmark
Sever, Dror; DanStem, University of Copenhagen, Copenhagen, Denmark
Karremore, Gopal; DanStem, University of Copenhagen, Copenhagen, Denmark
Novak, Ivana; Cell Biology and Physiology, Department of Biology, University of Copenhagen, Copenhagen, Denmark
Sneppen, Kim; Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark
Grapin-Botton, Anne ; DanStem, University of Copenhagen, Copenhagen, Denmark
Language :
English
Title :
Deconstructing the principles of ductal network formation in the pancreas.
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