Sa/So ratio; adsorbent; aflatoxin M1 carry-over; aflatoxins; bentonite; biomarkers; cocontamination; fumonisin esterase; fumonisins; General Agricultural and Biological Sciences; General Chemistry
Abstract :
[en] The objective of the study was to investigate the efficacy of bentonite and fumonisin esterase, separately or combined, in mitigating the effects of aflatoxins (AF) and fumonisins (FUM) in Boran and Friesian-Boran crossbreed cattle. These effects were studied by measuring mycotoxins, their metabolites, and biomarkers that relate to animal health, productivity, and food safety. The study was divided into three experiments each lasting for 2 weeks. Cows in experiment 1 received in random order aflatoxin B1 (AFB1) [788 μg/cow/day (69.7 μg/kg dry matter intake (DMI)) for Borans and 2,310 μg/cow/day (154 μg/kg DMI) for crossbreeds], bentonite (60 g/cow/day), or both AFB1 and bentonite. Boran cows in experiment 2 received in random order FUM (12.4 mg/cow/day (1.1 mg/kg DMI)), fumonisin esterase (120 U/cow/day), or both FUM and fumonisin esterase. Boran cows in experiment 3 received in random order AFB1 (952 μg/cow/day (84.2 μg/kg DMI)) + FUM (30.4 mg/cow/day (2.7 mg/kg DMI)), bentonite (60 g/cow/day) + fumonisin esterase (120 U/cow/day), or both AFB1 + FUM and bentonite + fumonisin esterase. Feeding AFB1 and/or FUM contaminated feed with or without the addition of the detoxifiers for 14 days did not affect DMI, milk composition, hematology, and blood biochemical parameters. The addition of bentonite in a diet contaminated with AFB1 led to a decrease in milk aflatoxin M1 (AFM1) concentration of 30% and 43%, with the carry-over subsequently decreasing from 0.35% to 0.20% and 0.08% to 0.06% for crosses and Borans, respectively. No significant change was observed in the sphinganine/sphingosine (Sa/So) ratio following feeding with FUM alone or in combination with fumonisin esterase; however, the ability of fumonisin esterase to hydrolyze FUM into less toxic fully hydrolyzed FUM and partially hydrolyzed FUM was evident in the rumen fluid and feces. These results indicate bentonite was effective in decreasing AFM1 concentration in milk, and AFB1 and AFM1 in plasma, while fumonisin esterase can convert FUM into less toxic metabolites and can be a suitable addition to feed cocontaminated with AFB1 and FUM.
Research center :
FARAH. Santé publique vétérinaire - ULiège
Disciplines :
Food science
Author, co-author :
Kemboi, David ; Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke 9820, Belgium ; Department of Veterinary Pathology, Microbiology, and Parasitology, Faculty of Veterinary Medicine, University of Nairobi, P.O Box 29053, Nairobi 00100, Kenya ; Department of Animal Science, Chuka University, P.O Box 109-00625, Chuka 00625, Kenya
Antonissen, Gunther; Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke 9820, Belgium ; Chair Poultry Health Sciences, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke 9820, Belgium
Ochieng, Phillis; Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke 9820, Belgium ; Department of Food Sciences, Faculty of Veterinary Medicine, University of Liège, Avenue de Cureghem 10, Liège 4000, Belgium
Croubels, Siska ; Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke 9820, Belgium
De Baere, Siegrid; Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke 9820, Belgium
Scippo, Marie-Louise ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Analyse des denrées alimentaires
Okoth, Sheila; Department of Biology, Faculty of Science and Technology, University of Nairobi, P.O Box 30197 Nairobi 00100, Kenya
Kangethe, Erastus; Consultant, P.O Box 34405, Nairobi 00100, Kenya
Faas, Johannes; DSM-BIOMIN Research Center, Technopark 1, Tulln 3430, Austria
Doupovec, Barbara; DSM-BIOMIN Research Center, Technopark 1, Tulln 3430, Austria
Lindahl, Johanna ; International Livestock Research Institute (ILRI), P.O Box 30709, Nairobi 00100, Kenya ; Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala SE-751 05, Sweden ; Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala SE-750 07, Sweden
Gathumbi, James; Department of Veterinary Pathology, Microbiology, and Parasitology, Faculty of Veterinary Medicine, University of Nairobi, P.O Box 29053, Nairobi 00100, Kenya
Language :
English
Title :
Efficacy of Bentonite and Fumonisin Esterase in Mitigating the Effects of Aflatoxins and Fumonisins in Two Kenyan Cattle Breeds.
Norges Forskningsråd [NO] BELSPO - Belgian Science Policy Office [BE] F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] NRF - National Research Foundation [ZA] Ministry of Education, Science and Technology [KE]
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