Escherichia coli O80 in Healthy Cattle: Absence of Shigatoxigenic and Enteropathogenic E. coli O80:H2 and (Phylo) Genomics of Non-Clonal Complex 165 E. coli O80
[en] The origin of human and calf infections by Shigatoxigenic (STEC) and enteropathogenic
(EPEC) Escherichia coli O80:H2 is still unknown. The aim of this study was to identify E. coli O80 in
healthy cattle with an emphasis on melibiose non-fermenting E. coli O80:H2. Faecal materials collected
from 149 bulls at 1 slaughterhouse and 194 cows on 9 farms were tested with O80 antigen-encoding
gene PCR after overnight growth in enrichment broths. The 53 O80 PCR-positive broths were streaked
on different (semi-)selective agar plates. Five E. coli colonies from 3 bulls and 11 from 2 cows tested
positive with the O80 PCR, but no melibiose non-fermenting E. coli was isolated. However, these
16 E. coli O80 were negative with PCR targeting the fliCH2, eae, stx1, stx2 and hlyF genes and were
identified by WGS to serotypes and sequence types O80:H6/ST8619 and O80:H45/ST4175. They
were phylogenetically related to E. coli O80:H6 and O80:H45 isolated from different animal species in
different countries, respectively, but neither to STEC and EPEC O80:H2/ST301, nor to other serotypes
of the clonal complex 165. As a conclusion, healthy adult cattle were not identified as a source of
contamination of humans and calves by STEC or EPEC O80:H2.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Ikeda, Rie ✱; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) ; Licensee MDPI, Basel, Switzerland
Thiry, Damien ✱; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie vétérinaire et maladies bactériennes animales ; Licensee MDPI, Basel, Switzerland
Mainil, Jacques ✱; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) ; Licensee MDPI, Basel, Switzerland
✱ These authors have contributed equally to this work.
Language :
English
Title :
Escherichia coli O80 in Healthy Cattle: Absence of Shigatoxigenic and Enteropathogenic E. coli O80:H2 and (Phylo) Genomics of Non-Clonal Complex 165 E. coli O80
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