Glycoproteins; Pregnancy Proteins; Animals; Breeding; Cattle; Female; Glycoproteins/blood; Goats; Pregnancy; Pregnancy Proteins/blood; Pregnancy Tests/methods; Pregnancy Tests/veterinary; Radioimmunoassay; Reproducibility of Results; Sensitivity and Specificity; Time Factors; PAG; Pregnancy diagnosis; Small Animals; Food Animals; Animal Science and Zoology; Equine; Pregnancy-associated glycoprotein
Abstract :
[en] Different RIA systems available for measuring the concentrations of pregnancy-associated glycoproteins (PAGs) in dairy goats were compared in order to evaluate their accuracy in early pregnancy diagnosis. Plasma concentrations of PAGs were determined by 3 heterologous RIA systems with a bovine PAG standard and tracer in combination with antisera anti-ovine PAG (RIA 1), anti-caprine PAG55 + 62 (RIA 2), anti-caprine PAG55 + 59 (RIA 3), and by 2 homologous RIA systems that employed caprine PAG55 + 62 and caprine PAG55 + 59 and their specific antisera (RIAs 4 and 5, respectively). In all of the RIAs, the mean concentrations of PAGs were significantly higher (P < 0.01) in pregnant than in nonpregnant goats from Day 21 onwards after breeding. On Day 21, the accuracy rates of early pregnancy diagnoses were 56% (RIA 1), 96% (RIA 2), 99% (RIA 3), 95% (RIA 4) and 90% (RIA 5), whereas on Day 28 these rates were > 99% for RIAs 2, 3, 4 and 5. The RIAs for PAGs depend on proteins from the placenta being present in maternal plasma and require only a single sample of blood, to distinguish pregnant goats from those that fail to return to estrus for other reasons. The homologous and semi-heterologous assays are highly accurate as early as Day 21 of pregnancy.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
González, F; Faculty of Veterinary, Las Palmas de Gran Canaria, Spain
Sulon, J; Physiology of Reproduction, Faculty of Veterinary Medicine, Liège, Belgium
Garbayo, J M; Physiology of Reproduction, Faculty of Veterinary Medicine, Liège, Belgium
Batista, M; Reproduction and Obstetrics, Faculty of Veterinary, Las Palmas de Gran Canaria, Spain
Cabrera, F; Reproduction and Obstetrics, Faculty of Veterinary, Las Palmas de Gran Canaria, Spain
Calero, P; Reproduction and Obstetrics, Faculty of Veterinary, Las Palmas de Gran Canaria, Spain
Gracia, A; Reproduction and Obstetrics, Faculty of Veterinary, Las Palmas de Gran Canaria, Spain
Beckers, Jean-François ; Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > Physiologie de la reproduction ; Physiology of Reproduction, Faculty of Veterinary Medicine, Liège, Belgium
Language :
English
Title :
Early pregnancy diagnosis in goats by determination of pregnancy-associated glycoprotein concentrations in plasma samples.
Acknowledgments This work wass upportedi,n part, by a grant from D.G. de Universidadees Investigationd el Gobiemod e Canariasto F. Gonzaleza nd Agriculture Ministry and FNRS grantst o J.F. BeckersT. he authorst hankJ .L. Alabart (AgriculturalResearchS ervice,A ragbn, Spain)f or statisticafa nalyseso f data, and Dr. R.M. Roberts( Departmento f Animal Sciencesa nd Biochemistry,U niversity of Missouri-Columbiaa)n d Dr. M. MC Namara( Biochemistry. Faculty of Medicine,U niversity of Liege)f or revisiono f the manuscript. “Reproductiona nd Obstetrics,F aculty of Veterinary, University of Las Palmasd e Gran Canaria3, 5416A rucas,L asP almasS, pain.
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