Effect of GnRH antagonist-induced prolonged follicular phase on follicular atresia and oocyte developmental competence in vitro in superovulated heifers.
Oussaid, B; Lonergan, P; Khatir, Het al.
2000 • In Journal of Reproduction and Fertility, 118 (1), p. 137 - 144
[en] A GnRH antagonist (Antarelix) was used to suppress endogenous pulsatile secretion of LH and delay the preovulatory LH surge in superovulated heifers to study the effect of a prolonged follicular phase on both follicle and oocyte quality. Oestrous cycles were synchronized in 12 heifers with progestagen (norgestomet) implants for 10 days. On day 4 (day 0 = day of oestrus), heifers were stimulated with 24 mg pFSH for 4 days and luteolysis was induced at day 6 with PGF2 alpha (2 ml Estrumate). Animals in the control group (n = 4) were killed 24 h after the last FSH injection. At this time, heifers in group A36h (n = 4) and group A60h (n = 4) were treated with 1.6 mg of Antarelix every 12 h for 36 and 60 h, respectively, and then killed. After dissection of ovarian follicles, oocytes were collected for individual in vitro maturation, fertilization and culture; follicular fluid was collected for determination of steroid concentrations, and granulosa cells were smeared, fixed and stained for evaluation of pycnosis rates. Granulosa cell smears showed that 90% of follicles were healthy in the control group. In contrast, 36 and 58% of the follicles in group A36h showed signs of early or advanced atresia, respectively, while 90% of the follicles in group A60h showed signs of late atresia. Intrafollicular concentrations of oestradiol decreased (P < 0.0001) from healthy follicles (799.14 +/- 40.65 ng ml-1) to late atretic follicles (3.96 +/- 0.59 ng ml-1). Progesterone concentrations were higher (P < 0.0001) in healthy follicles compared with atretic follicles, irrespective of degree of atresia. Oestradiol:progesterone ratios decreased (P < 0.0001) from healthy (4.58 +/- 0.25) to late atretic follicles (0.07 +/- 0.009). The intrafollicular concentrations of oestradiol and progesterone were significantly higher (P < 0.0001) in the control than in the treated groups. The oestradiol:progesterone ratio was higher (P < 0.0001) in the control (4.55 +/- 0.25) than in the A36h (0.40 +/- 0.05) and A60h (0.07 +/- 0.009) groups. Unexpectedly, the cleavage rate of fertilized oocytes, blastocyst rate and number of cells per blastocyst were not significantly different among control (85%, 41% and 95 +/- 8), A36h (86%, 56% and 93 +/- 5) and A60h (88%, 58% and 79 +/- 4) groups. In addition, there were no significant differences in the blastocyst rates from oocytes derived from healthy (45%), early atretic (54%), advanced atretic (57%) and late atretic follicles (53%). In conclusion, the maintenance of the preovulatory follicles in superovulated heifers with a GnRH antagonist induced more atresia and a decrease in oestradiol and progesterone concentrations. However, the developmental potential in vitro to day 8 of the oocytes recovered from these atretic follicles was not affected.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Oussaid, B; INRA, Unité Physiologie de la Reproduction des Mammiféres Domestiques, Nouzilly, France
Lonergan, P; INRA, U. Physiol. Repro. Mammiferes D., 37380 Nouzilly, France ; Dept. of Anim. Sci. and Production, University College Dublin, Lyons Research Farm, Newcastle, Co. Dublin, Ireland
Khatir, H; INRA, U. Physiol. Repro. Mammiferes D., 37380 Nouzilly, France
Guler, A; INRA, U. Physiol. Repro. Mammiferes D., 37380 Nouzilly, France
Monniaux, D; INRA, U. Physiol. Repro. Mammiferes D., 37380 Nouzilly, France
Touze, J L; INRA, U. Physiol. Repro. Mammiferes D., 37380 Nouzilly, France
Beckers, Jean-François ; Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > Physiologie de la reproduction ; INRA, U. Physiol. Repro. Mammiferes D., 37380 Nouzilly, France
Cognie, Y; INRA, U. Physiol. Repro. Mammiferes D., 37380 Nouzilly, France
Mermillod, P; INRA, U. Physiol. Repro. Mammiferes D., 37380 Nouzilly, France
Language :
English
Title :
Effect of GnRH antagonist-induced prolonged follicular phase on follicular atresia and oocyte developmental competence in vitro in superovulated heifers.
Publication date :
January 2000
Journal title :
Journal of Reproduction and Fertility
ISSN :
0022-4251
Publisher :
Journals of Reproduction and Fertility Ltd, England
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