Effect of different culture systems on adipocyte differentiation-related protein (ADRP) in bovine embryos.

ADRP quantification and localisation; Culture environment; Lipid droplet; Membrane Proteins; Perilipin-2; RNA, Messenger; Animals; Cattle/embryology; Coculture Techniques/veterinary; Embryo Culture Techniques/veterinary; Embryo, Mammalian/metabolism; Fertilization in Vitro; Gene Expression Regulation, Developmental/physiology; Membrane Proteins/genetics; Membrane Proteins/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; Sheep; Food Animals; Animal Science and Zoology; Endocrinology; General Medicine

Abstract :

[en] Bovine embryos cultured in serum-containing media abnormally accumulate lipid droplets, compared to their in vivo counterparts. The objective of this study was to investigate the effect of different culture systems on the mRNA expression and on the quantification and localisation of adipocyte differentiation-related protein (ADRP), a protein associated with lipid accumulation in bovine blastocysts. Two experiments were independently performed for ADRP mRNA expression analysis. In experiment A, blastocysts were produced in modified synthetic oviduct fluid (mSOF)+10% foetal calf serum (FCS), in coculture (bovine oviduct epithelial cells, Boec) and in ewe oviducts, whereas in experiment B, they were produced in mSOF+10μM docosahexaenoic acid (DHA) and in vivo. Control groups were also performed. ADRP mRNA expression was downregulated in the Boec, ewe oviduct and in vivo groups compared to the 10% FCS or DHA groups, respectively. Moreover, the expression of this protein was downregulated in the Boec group compared to the control group (P<0.05). A third experiment (experiment C) was performed to quantify and localise ADRP protein. Boec, in vivo and control groups were tested. After immunofluorescence staining followed by confocal microscopy analysis, embryonic ADRP was clearly localised around lipid droplets, indicating that ADRP is also a lipid droplet coat protein in bovine embryos. In conclusion, our results demonstrate that bovine embryos at the blastocyst stage expressed ADRP mRNA and protein, and that the embryonic culture system modified this expression.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Al Darwich, A; INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France, CNRS, UMR7247 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France, Université François Rabelais de Tours, F-37041 Tours, France, IFCE, F-37380 Nouzilly, France

Perreau, C; INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France, CNRS, UMR7247 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France, Université François Rabelais de Tours, F-37041 Tours, France, IFCE, F-37380 Nouzilly, France

Tsikis, G; INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France, CNRS, UMR7247 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France, Université François Rabelais de Tours, F-37041 Tours, France, IFCE, F-37380 Nouzilly, France

Coudert, E; INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France, CNRS, UMR7247 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France, Université François Rabelais de Tours, F-37041 Tours, France, IFCE, F-37380 Nouzilly, France

Touzé, J L; INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France, CNRS, UMR7247 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France, Université François Rabelais de Tours, F-37041 Tours, France, IFCE, F-37380 Nouzilly, France

Briant, E; INRA, UEPAO, F-37380 Nouzilly, France

Beckers, Jean-François ; Université de Liège - ULiège > Département des sciences fonctionnelles (DSF)

Mermillod, P; INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France, CNRS, UMR7247 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France, Université François Rabelais de Tours, F-37041 Tours, France, IFCE, F-37380 Nouzilly, France

Guignot, F; INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France, CNRS, UMR7247 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France, Université François Rabelais de Tours, F-37041 Tours, France, IFCE, F-37380 Nouzilly, France. Electronic address: Florence.guignot@tours.inra.fr

Language :

English

Title :

Effect of different culture systems on adipocyte differentiation-related protein (ADRP) in bovine embryos.

Publication date :

March 2014

Journal title :

Animal Reproduction Science

ISSN :

0378-4320

eISSN :

1873-2232

Publisher :

Elsevier, Netherlands

Volume :

145

Issue :

3-4

Pages :

105 - 113

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.elsevier.com/content/article/PII:S0378432014000244?httpAccept=text/xml

Funding text :

This study was funded by the French National Institute for Agricultural Research (Nouzilly, France) and supported by the Syrian University of Aleppo through fellowships of A. Al Darwich. The authors are grateful to D. Capo and his team at INRA Nouzilly for in vivo embryo production, G. Gomot, J. Cognié, L. Ouvrard and their team for embryo production in ewe oviducts and their care of the animals. We wish to thank sincerely A. Thelie and R. Dalbies-Tran for skillful help with PCR, C. Cebo for providing us MFGM and for her precious advice in Western blot detection and C. Briant for statistical help. We also thank M. Bertoldo for English revision of the manuscript.

Available on ORBi :

since 08 January 2023

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