Article (Scientific journals)
Experimental Neospora Caninum Infection in Pregnant Dairy Heifers Raises Concentrations of Pregnancy-Associated Glycoproteins 1 and 2 in Foetal Fluids.
Mur-Novales, R; López-Gatius, F; Serrano-Pérez, B et al.
2016In Reproduction in Domestic Animals, 51 (2), p. 282 - 286
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Keywords :
Pregnancy Proteins; Amniotic Fluid/chemistry; Animals; Cattle; Cattle Diseases/metabolism; Cattle Diseases/parasitology; Coccidiosis/metabolism; Coccidiosis/parasitology; Coccidiosis/veterinary; Female; Pregnancy; Pregnancy Complications, Parasitic/metabolism; Pregnancy Complications, Parasitic/veterinary; Pregnancy Proteins/chemistry; Pregnancy Proteins/metabolism; Neospora; Amniotic Fluid; Cattle Diseases; Coccidiosis; Pregnancy Complications, Parasitic; Biotechnology; Animal Science and Zoology; Endocrinology; Pregnancy-associated glycoprotein; Assay
Abstract :
[en] Plasma concentrations of PAG-1 are used for pregnancy diagnosis and as a marker of placental/foetal well-being, while those of PAG-2 may be an indicator of abortion risk in Neospora caninum-infected cows. Studies have shown that N. caninum infection modifies PAG-1 and PAG-2 patterns in maternal blood plasma. However, no prior work has examined the effects of N. caninum infection on concentrations of PAGs in foetal fluids. In this study, PAG-1, PAG-2 and pH levels were determined in the amniotic and allantoic fluids of foetuses collected at 152 days of gestation from control uninfected dams and from dams experimentally infected with N. caninum on Day 110 of gestation. Foetal fluids from infected foetuses had significantly higher PAG-2 concentrations (p = 0.026) and pH values (p = 0.02) than fluids from non-infected foetuses. In infected foetuses, significantly higher concentrations of PAG-1 (p < 0.001) and PAG-2 (p < 0.001) were detected in fluid samples showing antibodies against N. caninum than those without antibodies. Moreover, pH values were significantly higher (p = 0.011) in foetal fluid samples with antibodies than in samples from non-infected foetuses. In conclusion, this is the first report on the effect of N. caninum infection on PAG levels in foetal fluids. Our results indicate that following the experimental infection of dams with N. caninum on Day 110 of gestation, foetal fluids collected from the infected foetuses of these dams featured higher PAG-1 and PAG-2 levels and pH values than fluids from non-infected controls, provided that the samples tested showed the presence of antibodies. The clinical implications of these findings are that following infection with N. caninum, most cows will experience some level of placental damage and that this injury correlates with foetal fluid PAG levels and pH.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Mur-Novales, R;  Department of Animal Production, University of Lleida, Lleida, Spain
López-Gatius, F;  Agrotecnio Center, University of Lleida, Lleida, Spain
Serrano-Pérez, B;  Department of Animal Production, University of Lleida, Lleida, Spain ; Agrotecnio Center, University of Lleida, Lleida, Spain
García-Ispierto, I;  Department of Animal Production, University of Lleida, Lleida, Spain ; Agrotecnio Center, University of Lleida, Lleida, Spain
Darwich, L;  Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona (UAB), Cerdanyola del Vallès, Spain ; Centre de Recerca en Sanitat Animal (CReSA), Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Cerdanyola del Vallès, Spain
Cabezón, O;  SeFAS, UAB, Cerdanyola del Vallés, Spain, Spain
de Sousa, N M;  Physiology of Reproduction, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
Beckers, Jean-François  ;  Université de Liège - ULiège > Département des sciences fonctionnelles (DSF)
Almería, S;  Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona (UAB), Cerdanyola del Vallès, Spain ; Centre de Recerca en Sanitat Animal (CReSA), Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Cerdanyola del Vallès, Spain
Language :
English
Title :
Experimental Neospora Caninum Infection in Pregnant Dairy Heifers Raises Concentrations of Pregnancy-Associated Glycoproteins 1 and 2 in Foetal Fluids.
Publication date :
April 2016
Journal title :
Reproduction in Domestic Animals
ISSN :
0936-6768
eISSN :
1439-0531
Publisher :
Blackwell Publishing Ltd, Germany
Volume :
51
Issue :
2
Pages :
282 - 286
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
MINECO - Gobierno de Espana. Ministerio de Economia y Competitividad
FEDER - Federación Española de Enfermedades Raras
MICINN - Ministerio de Ciencia e Innovacion
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