Investigation of PAG2 mRNA Expression in Water Buffalo Peripheral Blood Mononuclear Cells and Polymorphonuclear Leukocytes from Maternal Blood at the Peri-Implantation Period.
PAG2 mRNA; peri-implantation; pregnancy diagnosis; water buffalo; Veterinary (all); General Veterinary; pregnancy-associated glycoprotein
Abstract :
[en] : The main objective of this study was to assess PAG2 mRNA expression in maternal blood cells at the peri-implantation period in water buffalo; moreover, we wanted to evaluate the earliest time in which PAG-2 could be detected in maternal blood. Thirty-two lactating buffaloes artificially inseminated (AI) were utilized. Blood was collected at Days 0, 14, 18, 28, 40 after AI (AI = day 0). Pregnancy was diagnosed by ultrasound at Days 28 and 40 post AI. Out of 32 buffaloes, 14 were pregnant (P group) and 18 were not pregnant (NP group). The plasma PAG-2 threshold of 1.0 ng/mL in the P group was reached at day 40 post AI. PAG2 mRNA expression differed between the P and NP groups, and was either evaluated in Peripheral Blood Mononuclear Cells (PBMC) or Polymorphonuclear Leukocytes (PMN), starting from day 14. However, both the estimated marginal means and multiple comparisons showed that PAG2 mRNA expression was higher in PMN than PBMC. In the present study, PAG-2 appeared in the blood (40 Days post AI), and an early expression of PAG2 mRNA at Day 14 post AI was also observed. Although further research is undoubtedly required, PAG2 mRNA in peripheral blood leukocytes could be using to better understand the role that PAGs play during pregnancy in buffalo.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Barbato, Olimpia ; Dipartimento di Medicina Veterinaria, Università degli studi di Perugia, 06100 Perugia, Italy. olimpia.barbato@unipg.it
Guelfi, Gabriella ; Dipartimento di Medicina Veterinaria, Università degli studi di Perugia, 06100 Perugia, Italy. gabriella.guelfi@unipg.it
Menchetti, Laura ; Dipartimento di Medicina Veterinaria, Università degli studi di Perugia, 06100 Perugia, Italy. laura.menchetti7@gmail.com
Brecchia, Gabriele; Dipartimento di Medicina Veterinaria, Università degli studi di Perugia, 06100 Perugia, Italy. gabriele.brecchia@unipg.it
de Sousa, Noelita Melo; Laboratoires d'Endocrinologie Animale et de Reproduction, Faculté de Médicine Vétérinaire, Université de Liège, B-4000 Liège, Belgium. noelitamelo@gmail.com
Canali, Claudio; Dipartimento di Medicina Veterinaria, Università degli studi di Perugia, 06100 Perugia, Italy. claudio.canali@unipg.it
Grandoni, Francesco; CREA Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca Zootecnia e Acquacoltura, 00015 Monterotondo (Roma), Italy. francesco.grandoni@crea.gov.it
Scatà, Maria Carmela; CREA Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca Zootecnia e Acquacoltura, 00015 Monterotondo (Roma), Italy. mariacarmela.scata@crea.gov.it
De Matteis, Giovanna; CREA Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca Zootecnia e Acquacoltura, 00015 Monterotondo (Roma), Italy. giovanna.dematteis@crea.gov.it
Casano, Anna Beatrice; Dipartimento di Medicina Veterinaria, Università degli studi di Perugia, 06100 Perugia, Italy. noelitamelo@gmail.com
Beckers, Jean-François ; Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > Physiologie de la reproduction
Barile, Vittoria Lucia; CREA Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca Zootecnia e Acquacoltura, 00015 Monterotondo (Roma), Italy. vittorialucia.barile@crea.gov.it
Language :
English
Title :
Investigation of PAG2 mRNA Expression in Water Buffalo Peripheral Blood Mononuclear Cells and Polymorphonuclear Leukocytes from Maternal Blood at the Peri-Implantation Period.
Publication date :
14 January 2019
Journal title :
Veterinary Sciences
eISSN :
2306-7381
Publisher :
MDPI Multidisciplinary Digital Publishing Institute, Switzerland
Funding: This study was supported by Scientific and Technological Research Program founded by Fondazione Cassa di Risparmio of Perugia and Sterling SPA.
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