Article (Scientific journals)
Discovering Novel Bioactivities of Controversial Food Additives by Means of Simple Zebrafish Embryotoxicity (ZET) Assays
Dinh Duy Thanh; Nguyen, Bich Ngoc; Van den Bossche, François et al.
2022In Toxics, 11 (1), p. 8
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Keywords :
food additives; bioactivities; zebrafish; embryos; food safety; developmental toxicology
Abstract :
[en] The rising concerns about controversial food additives’ potential hazardous properties require extensive yet animal-minimized testing strategies. Zebrafish embryos are the ideal in vivo model representing both human and environmental health. In this study, we exposed zebrafish embryos to eight controversial food additives. Our results indicate that Sodium Benzoate is a Cat.3 aquatic toxicant, while Quinoline Yellow is a strong teratogen. At high concentrations, non-toxic chemicals induced similar phenotypes, suggesting the impact of ionic strength and the applicability of the darkened yolk phenotype as an indicator of nephrotoxicity. Three food additives showed unpredicted bioactivities on the zebrafish embryos: Brilliant Blue could weaken the embryonic yolk, Quinoline Yellow may interfere with nutrient metabolism, and Azorubine induced precocious zebrafish hatching. In conclusion, the zebrafish embryo is ideal for high throughput chemical safety and toxicity screening, allowing systematic detection of biological effects—especially those unexpected by targeted in vitro and in silico models. Additionally, our data suggest the need to reconsider the safety status of food additives Quinoline Yellow, Brilliant Blue, Sodium Benzoate, and other controversial food additives in further studies, as well as pave the way to further applications based on the newly found properties of Brilliant Blue and Azorubine.
Research center :
GIGA I3-Laboratory for Organogenesis and Regeneration -ULiège
Disciplines :
Pharmacy, pharmacology & toxicology
Biochemistry, biophysics & molecular biology
Food science
Veterinary medicine & animal health
Author, co-author :
Dinh Duy Thanh  ;  Université de Liège - ULiège > GIGA ; Université de Liège - ULiège > Faculté des Sciences > Form. doct. sc. (bioch., biol. mol. cel., bioinf. - paysage) ; Université de Liège - ULiège > Faculté des Sciences > Doct. sc. (bioch., biol. mol. cel., bioinf. - paysage) ; Université de Liège - ULiège > Faculté des Sciences > Doct. scienc. (bioch. biol. moléc. cell. bioinf. mod.
Nguyen, Bich Ngoc  ;  Université de Liège - ULiège > Urban and Environmental Engineering  ; Université de Liège - ULiège > Département ArGEnCo > LEMA (Local environment management and analysis)
Van den Bossche, François;  UNamur - Université de Namur [BE] > Molecular Physiology Research Unit, Faculty of Medicine
Lai-Thanh, Nguyen ;  VNU University of Science > Department of Cell Biology, Faculty of Biology
Muller, Marc  ;  Université de Liège - ULiège > GIGA > GIGA I3 - Laboratory for Organogenesis and Regeneration
Language :
English
Title :
Discovering Novel Bioactivities of Controversial Food Additives by Means of Simple Zebrafish Embryotoxicity (ZET) Assays
Publication date :
22 December 2022
Journal title :
Toxics
eISSN :
2305-6304
Publisher :
MDPI AG
Special issue title :
Zebrafish as a Model for Pharmacological and Toxicological Research
Volume :
11
Issue :
1
Pages :
8
Peer reviewed :
Peer Reviewed verified by ORBi
Development Goals :
3. Good health and well-being
Name of the research project :
Test d'additifs alimentaires et de leurs propriétés biologiques sur cellules et sur les poissons zèbres
Funders :
WBI - Wallonie-Bruxelles International [BE]
Funding number :
CMP9.10; CMP8.24
Available on ORBi :
since 22 December 2022

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