Chronic caffeine use does not influence behavior and brain oxidative status in mice

Widely consumed in foods and as an energy supplement, caffeine has been studied given its pharmacological effects, especially on Central Nervous System (CNS). In this sense, the present study investigated whether and to what extent caffeine chronic use can influence the brain oxidative status and the behavioral activity of the C57BL/6 female mice. For this, fifteen animals were randomized into the following groups: Control (0.9% saline solution), Caf10 (10 mg/kg caffeine), and Caf50 (50 mg/kg caffeine). The animals received one daily caffeine dose by i.p route for 120 days. Twenty-four hours after the last administration, the animals were subjected to behavioral tests and euthanized. The blood was used by biochemical analysis and the brain to evaluate the oxidative status and micromineral levels. The caffeine did not influence the anthropometric parameters, lipid profile, and C- reactive protein levels. Further, superoxide dismutase (SOD) and glutathione-s- transferase (GST) activities maintained the same response profile. On the other hand, catalase (CAT) activity was decreased in both groups receiving caffeine compared to the control group. Despite this, malondialdehyde and carbonyl protein levels did not change among the groups, as well as the distribution micromineral levels. In the same way, no caffeine dose altered the findings of anxiety-like behaviors in the animals. Considering the time of caffeine administration, we believe that there was a cellular adaptation triggered by its use, tending to a protective effect on the brain.
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