[en] Herbivore-induced plant volatiles (HIPVs) play a key role in the interactions between plants and herbivorous insects, as HIPVs can promote or deter herbivorous insects’ behavior. While aphids are common and serious phloem-feeding pests in farmland ecosystems, little is known about how aphids use their sensitive olfactory system to detect HIPVs. In this study, the antennal transcriptomes of the aphid species Megoura crassicauda were sequenced, and expression level analyses of M. crassicauda odorant receptors (ORs) were carried out. To investigate the chemoreception mechanisms that M. crassicauda uses to detect HIPVs, we performed in vitro functional studies of the ORs using 11 HIPVs reported to be released by aphid-infested plants. In total, 54 candidate chemosensory genes were identified, among which 20 genes were ORs. McraOR20 and McraOR43 were selected for further functional characterization because their homologs in aphids were quite conserved and their expression levels in antennae of M. crassicauda were relatively high. The results showed that McraOR20 specifically detected cis-jasmone, as did its ortholog ApisOR20 from the pea aphid Acyrthosiphon pisum, while McraOR43 did not respond to any of the HIPV chemicals that were tested. This study characterized the ability of the homologous OR20 receptors in the two aphid species to detect HIPV cis-jasmone, and provides a candidate olfactory target for mediating aphid behaviors.
Disciplines :
Entomology & pest control
Author, co-author :
WANG, Bo ✱; Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity, Tianjin Normal University, Tianjin, China ; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
Huang, Tianyu ✱; Université de Liège - ULiège > TERRA Research Centre ; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China ; Guangdong Laboratory for Lingnan Modern Agriculture (Shenzhen Branch), Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China
YAO, Yuan; Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity, Tianjin Normal University, Tianjin, China
Francis, Frédéric ; Université de Liège - ULiège > TERRA Research Centre > Gestion durable des bio-agresseurs
YAN, Chun-cai; Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity, Tianjin Normal University, Tianjin, China
WANG, Gui-rong; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China ; Guangdong Laboratory for Lingnan Modern Agriculture (Shenzhen Branch), Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China
WANG, Bing; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
✱ These authors have contributed equally to this work.
Language :
English
Title :
A conserved odorant receptor identified from antennal transcriptome of Megoura crassicauda that specifically responds to cis-jasmone
Publication date :
July 2022
Journal title :
Journal of Integrative Agriculture
ISSN :
2095-3119
eISSN :
2352-3425
Publisher :
Editorial Department of Scientia Agricultura Sinica
This work was funded by the National Natural Science Foundation of China (31572072 and 31801994), the Shenzhen Science and Technology Program, China (KQTD20180411143628272), the Natural Science Foundation of Tianjin, China (18JCYBJC96100), and the Tianjin Normal University Foundation, China (135305JF79).
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