Acyrthosiphon pisum; odorant receptor; phylogenetic analysis; trans-2-hexen-1-al; two-electrode voltage clamp; Food Science; Biochemistry; Ecology; Food Animals; Animal Science and Zoology; Agronomy and Crop Science; Plant Science
Abstract :
[en] Pea aphid, Acyrthosiphon pisum, is a serious pest of many different leguminous plants, and it mainly relies on its odorant receptors (Ors) to discriminate among host species. However, less is known about the role that Ors play in the host plant location. In this study, we identified a novel conserved odorant receptor clade by phylogenetic analysis, and conducted the functional analysis of ApisOr23 in A. pisum. The results showed that the homologous Ors from A. pisum, Aphis glycines and Aphis gossypii share 94.28% identity in amino acid sequences. Moreover, conserved motifs were analyzed using the annotated homologous Or23 from eight aphid species, providing further proof of the high conservation level of the Or23 clade. According to the tissue expression pattern analysis, ApisOr23 was mainly expressed in the antennae. Further functional study using a heterologous Xenopus expression system revealed that ApisOr23 was tuned to five plant volatiles, namely trans-2-hexen-1-al, cis-2-hexen-1-ol, 1-heptanol, 4´-ethylacetophenone, and hexyl acetate. Among them, trans-2-hexen-1-al, which is one of the main volatile organic compounds released from legume plants, activated the highest response of ApisOr23. Our findings suggest that the conserved Or23 clade in most aphid species might play an important role in host plant detection.
Disciplines :
Entomology & pest control
Author, co-author :
Huang, Tianyu ✱; Université de Liège - ULiège > TERRA Research Centre ; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
ZHANG, Rui-bin ✱; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China ; Guangdong Laboratory for Lingnan Modern Agriculture (Shenzhen Branch), Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China
YANG, Lu-lu; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
CAO, Song; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
Francis, Frédéric ; Université de Liège - ULiège > TERRA Research Centre > Gestion durable des bio-agresseurs
WANG, Bing; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
WANG, Gui-rong; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China ; Guangdong Laboratory for Lingnan Modern Agriculture (Shenzhen Branch), Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China
✱ These authors have contributed equally to this work.
Language :
English
Title :
Identification and functional characterization of ApisOr23 in pea aphid Acyrthosiphon pisum
Publication date :
08 November 2020
Journal title :
Journal of Integrative Agriculture
ISSN :
2095-3119
eISSN :
2352-3425
Publisher :
Editorial Department of Scientia Agricultura Sinica
This work was funded by the National Natural Science Foundation of China (31572072 and 31725023), the Intergovernmental International Science, Technology and Innovation Cooperation Key Project, China (2019YFE0105800), and the Shenzhen Science and Technology Program, China (KQTD20180411143628272). The funders had no role in study design, data collection or analysis, decision to publish or preparation of the manuscript.
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