[en] Dietary sodium (Na) levels were related to the content of the eggshell matrix. We therefore speculated that dietary Na supplementation as sodium bicarbonate (NaHCO3) or sodium sulfate (Na2SO4) may improve eggshell quality. Additionally, dietary NaHCO3 or Na2SO4 supplementation may further affect eggshell quality in different ways due to differences in anions. This study investigated and compared the effects of dietary Na supplementation in either NaHCO3 or Na2SO4 form on laying performance, eggshell quality, ultrastructure and components in laying hens. A total of 576 29-week-old Hy-Line Brown laying hens were randomly allocated to 8 dietary treatments that were fed a Na-deficient basal diet (0.07% Na, 0.15% Cl) supplemented with Na2SO4 or NaHCO3 at 0.08, 0.18, 0.23 or 0.33% Na for 12 weeks. No differences were observed in laying production performance with dietary Na supplementation. Dietary Na supplementation resulted in quadratic increases of eggshell breaking strength in both Na2SO4 and NaHCO3 added groups (P < 0.05), and Na2SO4-fed groups had a quadratic increase in the eggshell ratio at week 12 (P < 0.05). Compared with supplementing 0.08% Na, dietary supplementation of 0.23% Na increased the effective thickness (P < 0.05) in both Na2SO4 and NaHCO3 added groups, but decreased the thickness and knob width of the mammillary layer (P < 0.05). A linear increase on the calcium content of the shell was only observed with Na supplementation from NaHCO3 (P < 0.05). No differences were observed in Na contents of the shell with dietary Na supplemented by both sources. Dietary Na addition had a quadratic increase on uronic acid contents of shell membrane in NaHCO3-fed groups (P < 0.05). Moreover, the sulfated glycosaminoglycan (GAG) contents of shell membranes increased linearly with dietary Na supplementation (P < 0.05). Dietary supplementation of 0.23% Na from Na2SO4 increased the sulfated GAG contents of calcified eggshell (P < 0.05). Additionally, compared with NaHCO3-fed groups, Na2SO4-fed groups had higher eggshell breaking strength, thickness, eggshell weight ratio, effective thickness and the sulfated GAG contents of calcified eggshell at week 12. Overall, dietary supplementation of NaHCO3 or Na2SO4 could increase eggshell breaking strength, which may be related to increased sulfated GAG contents in eggshell membranes and improved ultrastructure. Higher eggshell breaking strength, thickness and eggshell ratio could be obtained when the diet was supplemented with 0.23% Na from Na2SO4.
Disciplines :
Animal production & animal husbandry
Author, co-author :
Fu, Yu ; Université de Liège - ULiège > TERRA Research Centre ; Laboratory of Quality & Safety Risk Assessment for Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
Zhang, H.J.; Laboratory of Quality & Safety Risk Assessment for Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
Wu, S.G.; Laboratory of Quality & Safety Risk Assessment for Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
Zhou, J.M.; Laboratory of Quality & Safety Risk Assessment for Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
Qi, G.H.; Laboratory of Quality & Safety Risk Assessment for Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
Wang, J.; Laboratory of Quality & Safety Risk Assessment for Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
Language :
English
Title :
Dietary supplementation with sodium bicarbonate or sodium sulfate affects eggshell quality by altering ultrastructure and components in laying hens
None. This study was supported by Shandong Key Science and Technology Innovation Program (2019JZZY010704), National Key R&D Program of China (2016YFD0501202), China Agriculture Research System (CARS-40-K12) and Agricultural Science and Technology Innovation Program (ASTIP) of the Chinese Academy of Agricultural Sciences.
Balnave, D., Muheereza, S.K., Improving eggshell quality at high temperatures with dietary sodium bicarbonate. Poultry Science 76 (1997), 588–593.
Balnave, D., Yoselewitz, I., Dixon, R.J., Physiological changes associated with the production of defective eggshells by hens receiving sodium chloride in the drinking water. The British Journal of Nutrition 61 (1989), 35–43.
Cohen, I., Hurwitz, S., The response of blood ionic constituents and acid-base balance to dietary sodium, potassium and chloride in laying fowls. Poultry Science 53 (1974), 378–383.
Davison, S., Wideman, R.F., Excess sodium bicarbonate in the diet and its effect on Leghorn chickens. British Poultry Science 33 (1992), 859–870.
Dunn, I., Poultry breeding for egg quality: traditional and modern genetic approaches. Nys, Y., Bain, M., van Immerseel, F., (eds.) Improving the safety and quality of eggs and egg products, 2011, Woodhead Publishing, Cambridge, UK, 245–260.
Dunn, I.C., Rodríguez-Navarro, A.B., Mcdade, K., Schmutz, M., Preisinger, R., Waddington, D., Wilson, P.W., Bain, M.M., Genetic variation in eggshell crystal size and orientation is large and these traits are correlated with shell thickness and are associated with eggshell matrix protein markers. Animal Genetics 43 (2012), 410–418.
Faria, D.E.D., Junqueira, O.M., Sakomura, N.K., Santana, Á.E., Influence of different levels of energy, vitamin D3 and sodium: chloride ratio on performance and eggshell quality of laying hens. Revista Brasileira de Zootecnia 29 (2000), 467–475.
Farndale, R.W., Sayers, C.A., Barrett, A.J., A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures. Connective Tissue Research 9 (1982), 247–248.
Fathi, M.M., Zein El-Dein, A., El-Safty, S.A., Radwan, L.M., Using scanning electron microscopy to detect the ultrastructural variations in eggshell quality of Fayoumi and Dandarawi chicken breeds. International Journal of Poultry Science 6 (2007), 236–241.
Fernandez, M.S., Moya, A., Lopez, L., Arias, J.L., Secretion pattern, ultrastructural localization and function of extracellular matrix molecules involved in eggshell formation. Matrix Biology 19 (2001), 793–803.
Gezen, S.S., Eren, M., Deniz, G., The effect of different dietary electrolyte balances on eggshell quality in laying hens. Revista de Medicina Veterinaria 156 (2005), 491–497.
Ha, Y.W., Son, M.J., Yun, K.S., Kim, Y.S., Relationship between eggshell strength and keratan sulfate of eggshell membranes. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology 147 (2007), 1109–1115.
Jiang, M.J., Zhao, J.P., Jiao, H.C., Wang, X.J., Zhang, Q., Lin, H., Dietary supplementation with sodium bicarbonate improves calcium absorption and eggshell quality of laying hens during peak production. British Poultry Science 56 (2015), 740–747.
Junqueira, O.M., Costa, P.T., Miles, R.D., Harms, R.H., Interrelationship between sodium chloride, sodium bicarbonate, calcium, and phosphorus in laying hen diets. Poultry Science 63 (1984), 123–130.
Liu, Z., Zhang, F., Li, L., Li, G., He, W., Linhardt, R.J., Compositional analysis and structural elucidation of glycosaminoglycans in chicken eggs. Glycoconjugate Journal 31 (2014), 593–602.
Liu, Z., Sun, X., Cai, C., He, W., Zhang, F., Linhardt, R.J., Characteristics of glycosaminoglycans in chicken eggshells and the influence of disaccharide composition on eggshell properties. Poultry Science 95 (2016), 2879–2888.
Machnik, A., Neuhofer, W., Jantsch, J., Dahlmann, A., Tammela, T., Machura, K., Park, J., Beck, F.X., Muller, D.N., Derer, W., Goss, J., Ziomber, A., Dietsch, P., Wagner, H., Rooijen, N.V., Kurtz, A., Hilgers, K.F., Alitalo, K., Eckardt, K., Luft, F.C., Kerjaschki, D., Titze, J., Macrophages regulate salt-dependent volume and blood pressure by a vascular endothelial growth factor-C–dependent buffering mechanism. Nature Medicine 15 (2009), 545–552.
Makled, M.N., Charles, O.W., Eggshell quality as influenced by sodium bicarbonate, calcium source and photoperiod. Poultry Science 66 (1987), 705–712.
Nobakht, A., Shivazad, M., Chamany, M., Safameher, A.R., The effects of dietary electrolyte balance on the performance and eggshell quality in the early laying period. Pakistan Journal of Nutrition 6 (2007), 991–995.
Nys, Y., Guyot, N., Egg formation and chemistry. Nys, Y., Bain, M., van Immerseel, F., (eds.) Improving the safety and quality of eggs and egg products, 2011, Woodhead Publishing, Cambridge, UK, 83–126.
Özturk, E., Effects of sodium bicarbonate on the egg production, egg quality and some blood parameters in Japanese quails. Turkish Journal of Veterinary and Animal Sciences 23 (1999), 359–366.
Panheleux, M., Bain, M., Fernandez, M.S., Morales, I., Gautron, J., Arias, J.L., Solomon, S.E., Hincke, M.T., Nys, Y., Organic matrix component and ultrastructure of eggshell: a comparative study. British Poultry Science 40 (1999), 240–252.
Radwan, L.M., Eggshell quality: a comparison between Fayoumi, Gimieizah and Brown Hy-line strains for mechanical properties and ultrastructure of their eggshells. Animal Production Science 56 (2016), 908–912.
Solomon, S.E., The eggshell: strength, structure and function. British Poultry Science 51 (2010), 52–59.
Sugar, D., Agocs, R.I., Tatar, E., Toth, G., Horvath, P., Sulyok, E., Szabo, A.J., The contribution of skin glycosaminoglycans to the regulation of sodium homeostasis in rats. Physiological Research 67 (2018), 777–785.
Suzuki, S., Strominger, J.L., Enzymatic sulfation of mucopolysaccharides in hen oviduct I. transfer of sulfate from 3′-phosphoadenosine 5′-phosphosulfate to mucopolysaccharides. The Journal of Biological Chemistry 235 (1960), 257–266.
Van Toledo, B., Parsons, A.H., Combs, G.F., Role of ultrastructure in determining eggshell strength. Poultry Science 61 (1982), 569–572.
Wei, S.M., Yue, H.Y., Wu, S.G., Zhang, H.J., Wang, J., Qi, G.H., Study on the tolerance of Jinghong laying hens to dietary sodium sulfate. Chinese Journal of Animal Nutrition 27 (2015), 2493–2501.
Zhang, Y.N., Zhang, H.J., Wang, J., Yue, H.Y., Qi, X.L., Wu, S.G., Qi, G.H., Effect of dietary supplementation of organic or inorganic manganese on eggshell quality, ultrastructure, and components in laying hens. Poultry Science 96 (2017), 2184–2193.
Zhang, Y.N., Zhang, H.J., Wu, S.G., Wang, J., Qi, G.H., Dietary manganese supplementation modulated mechanical and ultrastructural changes during eggshell formation in laying hens. Poultry Science 96 (2017), 2699–2707.
