[en] Public concerns regarding animal welfare are changing the selection of rearing systems in laying hens. This study investigated the effects of rearing systems on eggshell quality, bone parameters and relative expression levels of genes related to bone remodeling in aged laying hens. A total of 2,952 55-day-old Jing Tint Six pullets were randomly assigned to place in the conventional caging system (CCS) or aviary system (AVS) and kept until 95 weeks of age. The AVS group delayed the decrease of eggshell quality and alleviated the symptoms of osteoporosis in the humerus rather than in the femur. Eggshell breaking strength, thickness, weight, weight ratio, stiffness and fracture toughness were decreased linearly with age (from 55 to 95 weeks of age, p < 0.05). The AVS group had higher eggshell breaking strength, stiffness and fracture toughness than the CCS group (p < 0.05). Higher total calcium and phosphorus per egg were presented in the AVS group at 95 weeks of age (p < 0.05). At 95 weeks of age, the AVS group had a humerus with higher weight, volume, length, midpoint perimeter, cortical index, fat-free dry weight, ash content, total calcium per bone, total phosphorus per bone, average bone mineral density, strength, stiffness and work to fracture compared to the CCS group (p < 0.05). Such differences did not appear in the femur. The relative expression levels of alkaline phosphatase (ALP) and osteocalcin (OCN) genes in the femur and hormone receptors (vitamin D receptor (VDR), estrogen receptor alpha (ERα) and fibroblast growth factor 23 (FGF23)) genes in the humerus were significantly upregulated (p < 0.05) in the AVS group. The level of tartrate-resistant acid phosphatase (TRAP) transcripts was also increased (p < 0.05) in the femur of the AVS group. Overall, compared with the CCS, the AVS alleviated the deterioration of eggshell and bone qualities of aged laying hens, which may be related to the changes in the expression of genes associated with bone remodeling.
Disciplines :
Animal production & animal husbandry
Author, co-author :
Fu, Yu ; Université de Liège - ULiège > TERRA Research Centre ; Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China
Wang, Jing; Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China
Chen, Gang; Key Laboratory of Bio-environmental Engineering, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing, China
Zhang, Hai-Jun; Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China
Wu, Shu-Geng; Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China
Li, Bao-Ming; Key Laboratory of Bio-environmental Engineering, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing, China
Qi, Guang-Hai; Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China
Language :
English
Title :
Effects of rearing systems on the eggshell quality, bone parameters and expression of genes related to bone remodeling in aged laying hens.
Earmarked Fund for China Agriculture Research System Agricultural Science and Technology Innovation Program
Funding text :
This study was supported by China Agriculture Research System (CARS-40) and the Agricultural Science and Technology Innovation Program (ASTIP) of CAAS. Apart from providing funds, they were not involved in the experiment design, data analysis or writing of this manuscript.The authors thank the other staffs of Nutritional Modulation Lab of Institute of Feed Research and the staffs of Shangzhuang Farm of China Agricultural University for their assistance in conducting the experiment. YF acknowledges support from the graduate student’s international exchange grant of Chinese Academy of Agricultural Sciences.
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