Fully automated 18F-fluorination of N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB) for indirect labelling of nanobodies.

Nagachinta, Surasa; Novelli, Paolo; Joyard, Yoann; Maindron, Nicolas; Riss, Patrick; Dammicco, Sylvestre

doi:10.1038/s41598-022-23552-8

Article (Scientific journals)

Fully automated 18F-fluorination of N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB) for indirect labelling of nanobodies.

Nagachinta, Surasa; Novelli, Paolo; Joyard, Yoann et al.

2022 • In Scientific Reports, 12 (1), p. 18655

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/296341

DOI
10.1038/s41598-022-23552-8

PubMed
36333403

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Keywords :

Fluorine Radioisotopes; Single-Domain Antibodies; Succinimides; Fluorides; Benzoates; Radiopharmaceuticals; Halogenation; Isotope Labeling/methods; Succinimides/chemistry; Benzoates/chemistry; Radiopharmaceuticals/chemistry; Positron-Emission Tomography/methods; Fluorine Radioisotopes/chemistry; Multidisciplinary

Abstract :

[en] N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB), a widely used labeling agent to introduce the 4-[18F]fluorobenzoyl-prosthetic group, is normally obtained in three consecutive steps from [18F]fluoride ion. Here, we describe an efficient one-step labeling procedure of [18F]SFB starting from a tin precursor. This method circumvents volatile radioactive side-products and simplifies automatization. [18F]SFB was obtained after HPLC purification in a yield of 42 + 4% and a radiochemical purity (RCP) > 99% (n = 6). In addition, we investigate the automation of the coupling of [18F]SFB to a nanobody (cAbBcII10, targeting β-lactamase enzyme) and purification by size exclusion chromatography (PD-10 desalting column) to remove unconjugated reagent. Production and use of [18F]SFB were implemented on a radiosynthesis unit (Neptis®). The fully automated radiosynthesis process including purification and formulation required 160 min of synthesis time. [18F]SFB-labeled nanobody was obtained in a yield of 21 + 2% (activity yield 12 + 1% non-decay corrected) and a radiochemical purity (RCP) of > 95% (n = 3). This approach simplifies [18F]SFB synthesis to one-step, enhances the yield in comparison to the previous report and enables the production of radiolabeled nanobody on the same synthesis module.

Research Center/Unit :

GIGA CRC (Cyclotron Research Center) In vivo Imaging-Aging & Memory - ULiège

Disciplines :

Chemistry
Radiology, nuclear medicine & imaging

Author, co-author :

Nagachinta, Surasa ; Université de Liège - ULiège > Département des sciences cliniques > Neuroimagerie des troubles de la mémoire et revalidation cognitive

Novelli, Paolo ; Université de Liège - ULiège > Département de chimie (sciences)

Joyard, Yoann; ORA Neptis, Philippeville, Belgium

Maindron, Nicolas; ORA Neptis, Philippeville, Belgium

Riss, Patrick ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie organique-nucléaire

Dammicco, Sylvestre ; Université de Liège - ULiège > Département de chimie (sciences)

Language :

English

Title :

Fully automated 18F-fluorination of N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB) for indirect labelling of nanobodies.

Publication date :

04 November 2022

Journal title :

Scientific Reports

eISSN :

2045-2322

Publisher :

Springer Science and Business Media LLC, England

Volume :

Issue :

Pages :

18655

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://www.nature.com/articles/s41598-022-23552-8.pdf

Name of the research project :

Biowin Program PIT of the Walloon Region

Funders :

BioWin - BioWin : pôle santé de Wallonie

Available on ORBi :

since 08 November 2022

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