[en] This study investigated the thyroidal response to administration of recombinant human thyroid stimulating hormone (rhTSH) by means of serum total thyroxine (TT(4)) concentration and pertechnetate uptake by the thyroid gland in six healthy euthyroid spayed female cats. A pertechnetate scan was performed on day 1 to calculate thyroid/salivary gland (T/S) uptake ratio. On day 3, 25 microg rhTSH was injected intravenously. Six hours later the thyroid scan was repeated as on day 1. Blood was drawn for serum TT(4) measurement prior to injection of rhTSH and performance of the pertechnetate scan. Statistically significant differences in mean serum TT(4) concentration, T/S uptake ratio before and 6h after rhTSH administration and T/S uptake ratio between left and right lobes were noted. We can conclude that 25 microg rhTSH increases pertechnetate uptake in the thyroid glands of cats, this should be taken into account when thyroid scintigraphy after rhTSH administration is interpreted.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
van Hoek, Ingrid M; Department of Medicine and Clinical Biology of Small Animals, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. ingrid.vanhoek@ugent.be
Peremans, Kathelijne; Department of Medical Imaging of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium
Vandermeulen, Eva; Department of Medical Imaging of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium
Duchateau, Luc; Department of Physiology and Biometrics, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium
Gommeren, Kris ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés (DCA) > Pathologie médicale des petits animaux ; Department of Medicine and Clinical Biology of Small Animals, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium
Daminet, Sylvie; Department of Medicine and Clinical Biology of Small Animals, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium
Language :
English
Title :
Effect of recombinant human thyroid stimulating hormone on serum thyroxin and thyroid scintigraphy in euthyroid cats.
Theon A.P., Van Vechten M.K., and Feldman E. Prospective randomized comparison of intravenous versus subcutaneous administration of radioiodine for treatment of hyperthyroidism in cats. Am J Vet Res 55 (1994) 1734-1738
Mooney C.T. Radioactive iodine therapy for feline hyperthyroidism: efficacy and administration routes. J Small Anim Pract 35 (1994) 289-294
Peterson M.E., and Becker D.V. Radioiodine treatment of 524 cats with hyperthyroidism. J Am Vet Med Assoc 207 (1995) 1422-1428
Chun R., Garrett L.D., Sargeant J., Sherman A., and Hoskinson J.J. Predictors of response to radioiodine therapy in hyperthyroid cats. Vet Radiol Ultrasound 43 (2002) 587-591
Nykamp S.G., Dykes N.L., Zarfoss M.K., and Scarlett J.M. Association of the risk of development of hypothyroidism after iodine 131 treatment with the pretreatment pattern of sodium pertechnetate Tc 99m uptake in the thyroid gland in cats with hyperthyroidism: 165 cases (1990-2002). J Am Vet Med Assoc 226 (2005) 1671-1675
Greco D.S. Diagnosis of congenital and adult-onset hypothyroidism in cats. Clin Tech Small Anim Pract 21 (2006) 40-44
Peterson M.E., Kintzer P.P., Cavanagh P.G., et al. Feline hyperthyroidism: pretreatment clinical and laboratory evaluation of 131 cases. J Am Vet Med Assoc 183 (1983) 103-110
Thoday K.L., and Mooney C.T. Historical, clinical and laboratory features of 126 hyperthyroid cats. Vet Rec 131 (1992) 257-264
Broussard J.D., Peterson M.E., and Fox P.R. Changes in clinical and laboratory findings in cats with hyperthyroidism from 1983 to 1993. J Am Vet Med Assoc 206 (1995) 302-305
Peterson M.E., and Gamble D.A. Effect of non-thyroidal illness on serum thyroxine concentrations in cats: 494 cases (1988). J Am Vet Med Assoc 197 (1990) 1203-1208
Hoenig M., and Ferguson D.C. Assessment of thyroid functional reserve in the cat by the thyrotropin-stimulation test. Am J Vet Res 44 (1983) 1229-1232
Kemppainen R.J., Mansfield P.D., and Sartin J.L. Endocrine responses of normal cats to TSH and synthetic ACTH administration. J Am Anim Hosp Assoc 20 (1984) 737-740
DiBartola S.P., and Tarr M.J. Corticotropin and thyrotropin response tests in Abyssinian cats with familial amyloidosis. J Am Anim Hosp Assoc 25 (1989) 217-220
Sparkes A.H., Jones B.R., Gruffyddjones T.J., and Walker M.J. Thyroid-function in the cat - assessment by the Trh response test and the thyrotropin stimulation test. J Small Anim Pract 32 (1991) 59-63
Mooney C.T., Thoday K.L., and Doxey D.L. Serum thyroxine and triiodothyronine responses of hyperthyroid cats to thyrotropin. Am J Vet Res 57 (1996) 987-991
Ribela M.T., Bianco A.C., and Bartolini P. The use of recombinant human thyrotropin produced by Chinese hamster ovary cells for the preparation of immunoassay reagents. J Clin Endocrinol Metab 81 (1996) 249-256
Sauvé F., and Paradis M. Use of recombinant human thyroid-stimulating hormone for thyrotropin stimulation test in euthyroid dogs. Can Vet J 41 (2000) 215-219
Boretti F.S., Sieber-Ruckstuhl N.S., Favrot C., et al. Evaluation of recombinant human thyroid-stimulating hormone to test thyroid function in dogs suspected of having hypothyroidism. Am J Vet Res 67 (2006) 2012-2016
Boretti F.S., Sieber-Ruckstuhl N.S., Willi B., Lutz H., Hofmann-Lehmann R., and Reusch C.E. Comparison of the biological activity of recombinant human thyroid-stimulating hormone with bovine thyroid-stimulating hormone and evaluation of recombinant human thyroid-stimulating hormone in healthy dogs of different breeds. Am J Vet Res 67 (2006) 1169-1172
Daminet S., Fifle L., Paradis M., Duchateau L., and Moreau M. Use of recombinant human thyroid-stimulating hormone for thyrotropin stimulation test in healthy, hypothyroid and euthyroid sick dogs. Can Vet J 48 (2007) 1273-1279
Stegeman J.R., Graham P.A., and Hauptman J.G. Use of recombinant human thyroid-stimulating hormone for thyrotropin-stimulation testing of euthyroid cats. Am J Vet Res 64 (2003) 149-152
Mooney C.T., Thoday K.L., Nicoll J.J., and Doxey D.J. Qualitative and quantitative thyroid imaging in feline hyperthyroidism using technetium-99m as pertechnetate. Vet Radiol Ultrasound 33 (1992) 313-320
Daniel G.B., Sharp D.S., Nieckarz J.A., and Adams W. Quantitative thyroid scintigraphy as a predictor of serum thyroxin concentration in normal and hyperthyroid cats. Vet Radiol Ultrasound 43 (2003) 374-382
De Roover K., Duchateau L., Carmichael N., van Geffen C., and Daminet S. Effect of storage of reconstituted recombinant human thyroid-stimulating hormone (rhTSH) on thyroid-stimulating hormone (TSH) response testing in euthyroid dogs. J Vet Intern Med 20 (2006) 812-817
Ekholm R., Engstrom G., Ericson L.E., and Melander A. Exocytosis of protein into the thyroid follicle lumen: an early effect of TSH. Endocrinology 97 (1975) 337-346
Collins W.T., and Capen C.C. Ultrastructural and functional alterations of the rat thyroid gland produced by polychlorinated biphenyls compared with iodide excess and deficiency, and thyrotropin and thyroxine administration. Virchows Arch B Cell Pathol Incl Mol Pathol 33 (1980) 213-231
Nilsson M., Engstrom G., and Ericson L.E. Graded response in the individual thyroid follicle cell to increasing doses of TSH. Mol Cell Endocrinol 44 (1986) 165-169
Kogai T., Endo T., Saito T., Miyazaki A., Kawaguchi A., and Onaya T. Regulation by thyroid-stimulating hormone of sodium/iodide symporter gene expression and protein levels in FRTL-5 cells. Endocrinology 138 (1997) 2227-2232
Lorenz M.D., and Stiff M.E. Serum thyroxine content before and after thyrotropin stimulation in dogs with suspected hypothyroidism. J Am Vet Med Assoc 177 (1980) 78-81
Thotakura N.R., Desai R.K., Szkudlinski M.W., and Weintraub B.D. The role of the oligosaccharide chains of thyrotropin alpha- and beta-subunits in hormone action. Endocrinology 131 (1992) 82-88
Rayalam S., Eizenstat L.D., Hoenig M., and Ferguson D.C. Cloning and sequencing of feline thyrotropin (fTSH): heterodimeric and yoked constructs. Domest Anim Endocrinol 30 (2006) 203-217
Nunez M.R., Sanders J., Jeffreys J., et al. Analysis of the thyrotropin receptor-thyrotropin interaction by comparative modeling. Thyroid 14 (2004) 991-1011
Szkudlinski M.W., Thotakura N.R., Bucci I., et al. Purification and characterization of recombinant human thyrotropin (TSH) isoforms produced by Chinese hamster ovary cells: the role of sialylation and sulfation in TSH bioactivity. Endocrinology 133 (1993) 1490-1503
Nieckarz J.A., and Daniel G.B. The effect of methimazole on thyroid uptake of pertechnetate and radioiodine in normal cats. Vet Radiol Ultrasound 42 (2001) 448-457
Lambrechts N., Jordaan M.M., Pilloy W.J., van Heerden J., and Clauss R.P. Thyroidal radioisotope uptake in euthyroid cats: a comparison between 131I and 99mTcO4. J S Afr Vet Assoc 68 (1997) 35-39
Jhiang S.M., Cho J.Y., Ryu K.Y., et al. An immunohistochemical study of Na+/I- symporter in human thyroid tissues and salivary gland tissues. Endocrinology 139 (1998) 4416-4419
Spitzweg C., Joba W., Eisenmenger W., and Heufelder A.E. Analysis of human sodium iodide symporter gene expression in extrathyroidal tissues and cloning of its complementary deoxyribonucleic acids from salivary gland, mammary gland, and gastric mucosa. J Clin Endocrinol Metab 83 (1998) 1746-1751
De La Vieja A., Dohan O., Levy O., and Carrasco N. Molecular analysis of the sodium/iodide symporter: impact on thyroid and extrathyroid pathophysiology. Physiol Rev 80 (2000) 1083-1105
Scrivani P.V., Dykes N.L., Page R.B., and Erb H.N. Investigation of two methods for assessing thyroid-lobe asymmetry during pertechnetate scintigraphy in suspected hyperthyroid cats. Vet Radiol Ultrasound 48 (2007) 383-387
Wisner E.R., Theon A.P., Vet M., Nyland T.G., and Hornof W.J. Ultrasonographic examination of the thyroid gland of hyperthyroid cats: a comparison to 99mTcO4- scintigraphy. Vet Radiol Ultrasound 35 (1994) 53-58
Balogh L., Thuroczy J., Biksi I., et al. Thyroid volumetric measurement and quantitative thyroid scintigraphy in dogs. Acta Vet Hung 46 (1998) 145-156
Yildirim M., Dane S., and Seven B. Morphological asymmetry in thyroid lobes, and sex and handedness differences in healthy young subjects. Int J Neurosci 116 (2006) 1173-1179
Gerendai I., and Halasz B. Neuroendocrine asymmetry. Front Neuroendocrinol 18 (1997) 354-381
Gontova I.A., Abramov V.V., and Kozlov V.A. Asymmetry of delayed type hypersensitivity reaction in mice. Bull Exp Biol Med 135 (2003) 67-69
Huysmans D.A., Nieuwlaat W.A., Erdtsieck R.J., et al. Administration of a single low dose of recombinant human thyrotropin significantly enhances thyroid radioiodide uptake in nontoxic nodular goiter. J Clin Endocrinol Metab 85 (2000) 3592-3596
Nieuwlaat W.A., Hermus A.R., Sivro-Prndelj F., Corstens F.H., and Huysmans D.A. Pretreatment with recombinant human TSH changes the regional distribution of radioiodine on thyroid scintigrams of nodular goiters. J Clin Endocrinol Metab 86 (2001) 5330-5336
Nieuwlaat W.A., Huysmans D.A., van den Bosch H.C., et al. Pretreatment with a single, low dose of recombinant human thyrotropin allows dose reduction of radioiodine therapy in patients with nodular goiter. J Clin Endocrinol Metab 88 (2003) 3121-3129
Duick D.S., and Baskin H.J. Significance of radioiodine uptake at 72 hours versus 24 hours after pretreatment with recombinant human thyrotropin for enhancement of radioiodine therapy in patients with symptomatic non-toxic or toxic multinodular goiter. Endocr Pract 10 (2004) 253-260
Nieuwlaat W.A., Hermus A.R., Ross H.A., et al. Dosimetry of radioiodine therapy in patients with nodular goiter after pretreatment with a single, low dose of recombinant human thyroid-stimulating hormone. J Nucl Med 45 (2004) 626-633
Feeney D.A., Jessen C.R., Weichselbaum R.C., Cronk D.E., and Anderson K.L. Relationship between orally administered dose, surface emission rate for gamma radiation, and urine radioactivity in radioiodine-treated hyperthyroid cats. Am J Vet Res 64 (2003) 1242-1247
Weichselbaum R.C., Feeney D.A., and Jessen C.R. Evaluation of relationships between pretreatment patient variables and duration of isolation for radioiodine-treated hyperthyroid cats. Am J Vet Res 64 (2003) 425-427
