[en] We investigated the effects of punctual A-to-V and A-to-T mutations in the amyloid precursor protein APP, corresponding to position 2 of Aβ1-42. Those mutations had opposite effects on the onset and progression of Alzheimer disease, the former inducing early AD pathology and the latter protecting against the onset of the disease. We applied Static and Dynamic Light Scattering and Circular Dichroism, to study the different mutants in the early stages of the aggregation process, essential for the disease. Comparative results showed that the aggregation pathways differ in the kinetics and extent of the process, in the size of the aggregates and in the evolution of the secondary structure, resulting in fibrils of different morphology, as seen by AFM. Mutated peptides had comparable toxic effects on N2a cells. Moreover, as assessed by X-ray scattering, all of them displayed disordering effects on the internal structure of mixed phospholipids-gangliosides model membranes.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Colombo, Laura; Department of Molecular Biochemistry and Pharmacology, IRCCS Istituto di Ricerche Farmacologiche "Mario Negri", Via La Masa 19, 20156 Milan, Italy
Gamba, Alessio ; Université de Liège - ULiège > GIGA > GIGA In silico medecine - Biomechanics Research Unit ; Department of Medical Biotechnology and Translational Medicine, University of Milan, V.le F.lli Cervi 93, 20090 Segrate, Italy
Cantù, Laura; Department of Medical Biotechnology and Translational Medicine, University of Milan, V.le F.lli Cervi 93, 20090 Segrate, Italy
Salmona, Mario; Department of Molecular Biochemistry and Pharmacology, IRCCS Istituto di Ricerche Farmacologiche "Mario Negri", Via La Masa 19, 20156 Milan, Italy
Tagliavini, Fabrizio; Neurology V & Neuropathology, IRCCS Foundation "Carlo Besta" Neurological Institute, Via Celoria 11, 20133 Milan, Italy
Rondelli, Valeria; Department of Medical Biotechnology and Translational Medicine, University of Milan, V.le F.lli Cervi 93, 20090 Segrate, Italy
Del Favero, Elena; Department of Medical Biotechnology and Translational Medicine, University of Milan, V.le F.lli Cervi 93, 20090 Segrate, Italy. Electronic address: elena.delfavero@unimi.it
Brocca, Paola; Department of Medical Biotechnology and Translational Medicine, University of Milan, V.le F.lli Cervi 93, 20090 Segrate, Italy
Language :
English
Title :
Pathogenic Aβ A2V versus protective Aβ A2T mutation: Early stage aggregation and membrane interaction.
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