high-throughput sequencing; plant pest diagnostics; reference material; test performance study; training; validation
Abstract :
Ensuring the reliability of diagnostic activities is an essential cornerstone of plant health
strategies to reduce the risk of entry and spread of plant pests in a region and ultimately
their impacts. Diagnostic tests should be validated to ensure that they are fit for purpose.
Validation is usually done by diagnostic laboratories, although companies commercializing
diagnostic kits also produce validation data for their products. Due to the high number of
pest,matrix, and method combinations and given the significant resources required to validate
tests, it is essential that validation data are shared with the entire diagnostic community
and produced in a harmonized way to facilitate their use by different stakeholders. Indeed,
the selection of tests to be used in specific contexts is not the sole responsibility of diagnostic
laboratories but also involves national plant protection organizations.The VALITEST
EU project (2018 to 2021) was established to tackle all these issues.New validation data for
tests targeting important pests for the European and Mediterranean Plant Protection Organization
region were produced. Guidelines to improve and harmonize the validation framework
were developed. Sharing of validation data and experience was ensured through the
development of new or existing databases, the organization of training courses, and the
dissemination of the project outputs in scientific publications and standards. Finally, the
involvement of researchers, diagnosticians, policy makers, inspectors, and industries and
the establishment of the European Plant Diagnostic Industry Association were important
actions to strengthen the interactions between plant health stakeholders
Disciplines :
Biotechnology
Author, co-author :
Trontin, Charlotte; European, Mediterranean Plant Protection Organization (EPPO), 21 Bd Richard Lenoir, 75011 Paris, France
Agstner, Barbara; Fera Science Ltd., York Biotech Campus, Sand Hutton, York, YO41 1LZ, U.K.
Altenbach, Denise; Agroscope,Research Division, Plant Protection, Phytopathology and Zoology in Fruit and Vegetable Production, Wädenswil, 8820, Switzerland
Anthoine, Geraldine; French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Plant Health Laboratory, 7 rue Jean Dixméras, 49044 Angers, France
Baginska, Hanna; Main Inspectorate of Plant Health and Seed Inspection, ˙ Zwirki i Wigury 73 str., 87-100 Toru´ n, Poland
Brittain, Ian; Fera Science Ltd., York Biotech Campus, Sand Hutton, York, YO41 1LZ, U.K.
Chabirand, Aude; French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Plant Health Laboratory, Pests and Tropical Pathogens Unit, Pôle de Protection des Plantes, Bât.CIRAD, Ligne Paradis, 7 chemin de l’IRAT, 97410 Saint Pierre, France
Chappé, Anne-Marie; French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Plant Health Laboratory, Nematology unit, Domaine de la Motte au Vicomte, BP 35327, 35653 Le Rheu Cedex, France
Dahlin, Paul; Agroscope,Research Division, Plant Protection, Phytopathology and Zoology in Fruit and Vegetable Production, Wädenswil, 8820, Switzerland
Dreo, Tanja; National Institute of Biology, Veˇcna pot 111, 1000 Ljubljana, Slovenia
Gianinazzi, Camilo; International Plant Analysis and Diagnostics srl (IpadLab), Via Einstein, SNC - Cascina Codazza, 26900 Lodi, Italy
Harrison, Catherine; Fera Science Ltd., York Biotech Campus, Sand Hutton, York, YO41 1LZ, U.K.
Jones, Glyn; Fera Science Ltd., York Biotech Campus, Sand Hutton, York, YO41 1LZ, U.K.
Kaiser, Marco Stefan; BIOREBA AG, Christoph Merian Ring 7,CH - 4153 Reinach, Switzerland
Massart, Sébastien ; Université de Liège - ULiège > TERRA Research Centre > Gestion durable des bio-agresseurs
Luigi, Marta; Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA),Centro di Ricerca Difesa e Certificazione, Via C.G. Bertero, 22, 00156 Rome, Italy
Mehle, Natasa; National Institute of Biology, Veˇcna pot 111, 1000 Ljubljana, Slovenia and University of Nova Gorica, School for Viticulture and Enology, Dvorec Lanthieri Glavni trg 8, 5271 Vipava, Slovenia
Mezzalama, Monica; GROINNOVA - Centre of Competence University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
Mouaziz, Hanna; French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Plant Health Laboratory, 7 rue Jean Dixméras, 49044 Angers, France
Ravnikar, Maja; National Institute of Biology, Veˇcna pot 111, 1000 Ljubljana, Slovenia
Raaymakers, Tom; Dutch National Plant Protection Organization, National Reference Centre,Geertjesweg 15, 6706EA, Wageningen, The Netherlands
Renvoisé, Jean-Philippe; French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Plant Health Laboratory, Quarantine Unit, 6 rue Aimé Rudel, Marmilhat, 63370 Lempdes, France
Rolland, Mathieu; French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Plant Health Laboratory, 7 rue Jean Dixméras, 49044 Angers, France