Osteoblast Transcriptome in 4 days old zebrafish larvae and the role of Fbln1 and Col10a1a bone extracellular matrix proteins during skeletal development
[en] Understanding osteoblast differentiation and their function in bone matrix
deposition and mineralization is central to the comprehension of bone development and of
various bone pathologies. It is, therefore, crucial to not only follow the expression of some
landmark transcription factors or ECM proteins, but to also investigate the status of
signaling pathways and factors regulating these processes. This can only be achieved by
assessing the entire transcriptome of these cells. The aim is to study the osteoblast
transcriptome, focusing on the possible existence of specific subpopulations at 4dpf and to investigate the role of efemp1, fbln1 and col10a1a genes that encode bone ECM proteins in zebrafish skeletal development indicated in the osteoblast transcriptomic data.
Research center :
GIGA I3-Laboratory for Organogenesis and Regeneration -ULiège GIGA Platform Genomics GIGA Platform Zebrafish Facility & Transgenics - ULiège GIGA CRC (Cyclotron Research Center) In vivo Imaging-Aging & Memory - ULiège INSERM U1132 Bioscar and Universite de Paris Cite, Reference Centre for Rare Bone Diseases, Hospital Lariboisiere, F-75010, Paris, France
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Raman, Ratish ; Université de Liège - ULiège > GIGA > GIGA I3 - Laboratory for Organogenesis and Regeneration
Salomao D’Cruz Alison; GIGA-I3, University of Liege > Laboratory of Organogenesis and Regeneration
Dikoum Cyrille; GIGA-I3, University of Liege > Laboratory of Organogenesis and Regeneration
Zappia, Jérémie ; Université de Liège - ULiège > Département des sciences de la motricité > musculoSkeletal Innovative research Lab (mSKIL)
Anthony Mishal; GIGA-I3, University of Liege > Laboratory of Organogenesis and Regeneration
Caetano da Silva Caroline; INSERM U1132 Bioscar and Universite de Paris Cite > Reference Centre for Rare Bone Diseases, Hospital Lariboisiere, F-75010, Paris, France
Nivelle, Renaud ; Université de Liège - ULiège > GIGA > GIGA I3 - Laboratory for Organogenesis and Regeneration
Degueldre Christian; University of Liege, GIGA CRC In Vivo Imaging, GIGA Platform, Sart Tilman Campus, 4000 Liege, Belgium
Ostertag Agnes; INSERM U1132 Bioscar and Universite de Paris-Cite, > Reference Centre for Rare Bone Diseases, Hospital Lariboisiere, F-75010, Paris, France
Bahri, Mohamed Ali ; Université de Liège - ULiège > GIGA > GIGA CRC In vivo Imaging - Aging & Memory
Renn, Jörg ; ULiège - University of Liège [BE] > Laboratory of Organogenesis and Regeneration
Collet Corrine; INSERM U1132 Bioscar and Universite de Paris-Cite, > Reference Centre for Rare Bone Diseases, Hospital Lariboisiere, F-75010, Paris, France
Plenevaux, Alain ; Université de Liège - ULiège > GIGA > GIGA CRC In vivo Imaging - Preclinical Imaging
Solal Cohen Martine; INSERM U1132 Bioscar and Universite de Paris Cite, > Reference Centre for Rare Bone Diseases, Hospital Lariboisiere, F-75010, Paris, France
Henrotin, Yves ; Université de Liège - ULiège > Département des sciences de la motricité > musculoSkeletal Innovative research Lab (mSKIL)
Muller, Marc ; Université de Liège - ULiège > GIGA > GIGA I3 - Laboratory for Organogenesis and Regeneration
Osteoblast Transcriptome in 4 days old zebrafish larvae and the role of Fbln1 and Col10a1a bone extracellular matrix proteins during skeletal development
Publication date :
07 September 2022
Event name :
German Society for Matrix biology (Deutsche Gesellschaft fuer Matrixbiologie e.V.) DGMB 2022
Event organizer :
Frank Zaucke, Uniklinikum Frankfurt am Main, Germany
Event place :
Frankfurt am Main, Germany
Event date :
07/09/2022
Audience :
International
European Projects :
H2020 - 766347 - BioMedaqu - Aquaculture meets Biomedicine: Innovation in Skeletal Health research.
Funders :
MSCA-ITN H2020 BioMedaqu Project, Fonds de la Recherche Scientifique (FNRS) and Fondation Arthrose "Jean Henrotin Prize"
Funding number :
ETN 766347
Funding text :
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 766347.
