Article (Scientific journals)
Monitoring and Occurrence of Heavy PAHs in Pomace Oil Supply Chain Using a Double-Step Solid-Phase Purification and HPLC-FLD Determination
Barp, Laura; Moret, Sabrina; Purcaro, Giorgia
2022In Foods, 11 (18), p. 2737
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Keywords :
polycyclic aromatic hydrocarbons (PAHs); olive oil; pomace oil; solid-phase extraction(SPE); high performance liquid chromatography-fluorometric detector (HPLC-FLD)
Abstract :
[en] Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental and processing contaminants generated by both spontaneous and anthropogenic incomplete combustion processes of organic matter. Contamination of PAHs in vegetable oils can result from several factors and processes, including environmental contamination, oil processing, and migration from food contact materials. The determination of PAHs in edible oil presents a challenge because of the complexity of the matrix. Since PAHs are present at lower levels than triglycerides, it is necessary to isolate the compounds of interest from the rest of the matrix. To this purpose, a new purification approach based on a double solid-phase extraction (SPE) step followed by high performance liquid chromatography–fluorometric detector (HPLC-FLD) analysis was developed. The method involves a first purification step by using a 5 g silica SPE cartridge, previously washed with dichloromethane (20 mL), dried completely, and then conditioned with n-hexane (20 mL). The triglycerides are retained by the silica, while the PAH-containing fraction is eluted with a mixture of n-hexane/dichloromethane (70/30, v/v). After evaporation, the residue is loaded on a 5 g amino SPE cartridge and eluted with n-hexane/toluene (70/30, v/v) before HPLC-FLD analysis. The focus was the evaluation of the contribution of the various phases of the pomace oil supply chain in terms of the heavy PAHs (PAH8) concentration. Data collected showed that pomace contamination increased (by 15 times) as storage time increased. In addition, the process of pomace drying, which is necessary to reduce its moisture content before solvent extraction of the residual oil, appeared to significantly contribute to the total heavy PAHs content, with increases in value by up to 75 times.
Disciplines :
Chemistry
Food science
Author, co-author :
Barp, Laura
Moret, Sabrina
Purcaro, Giorgia  ;  Université de Liège - ULiège > TERRA Research Centre > Chimie des agro-biosystèmes
Language :
English
Title :
Monitoring and Occurrence of Heavy PAHs in Pomace Oil Supply Chain Using a Double-Step Solid-Phase Purification and HPLC-FLD Determination
Publication date :
06 September 2022
Journal title :
Foods
eISSN :
2304-8158
Publisher :
MDPI AG
Special issue title :
Rapid Detection Methods for Food Fraud and Food Contaminants Series II
Volume :
11
Issue :
18
Pages :
2737
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 September 2022

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