[en] A promising keratin-degrading strain from the genus Chryseobacterium (Chryseobacterium sp. KMC2) was investigated using comparative genomic tools against three publicly available reference genomes to reveal the keratinolytic potential for biosynthesis of valuable secondary metabolites. Genomic features and metabolic potential of four species were compared, showing genomic differences but similar functional categories. Eleven different secondary metabolite gene clusters of interest were mined from the four genomes successfully, including five common ones shared across all genomes. Among the common metabolites, we identified gene clusters involved in biosynthesis of flexirubin-type pigment, microviridin, and siderophore, showing remarkable conservation across the four genomes. Unique secondary metabolite gene clusters were also discovered, for example, ladderane from Chryseobacterium sp. KMC2. Additionally, this study provides a more comprehensive understanding of the potential metabolic pathways of keratin utilization in Chryseobacterium sp. KMC2, with the involvement of amino acid metabolism, TCA cycle, glycolysis/gluconeogenesis, propanoate metabolism, and sulfate reduction. This work uncovers the biosynthesis of secondary metabolite gene clusters from four keratinolytic Chryseobacterium species and shades lights on the keratinolytic potential of Chryseobacterium sp. KMC2 from a genome-mining perspective, can provide alternatives to valorize keratinous materials into high-value bioactive natural products.
Disciplines :
Microbiology
Author, co-author :
Kang, Dingrong ; Université de Liège - ULiège > Département GxABT > Microbial technologies ; Section of Microbiology, Department of Biology, University of Copenhagen, 2100 Copenhagen, Denmark ; Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK ; Department of Bioengineering, Imperial College London, London SW7 2AZ, UK ; Centre for Host-Microbiome Interactions, Faculty of Dentistry, Oral & Craniofacial Sciences, King's College London, Lodon SE1 9RT, UK
Shoaie, Saeed; Centre for Host-Microbiome Interactions, Faculty of Dentistry, Oral & Craniofacial Sciences, King's College London, Lodon SE1 9RT, UK ; Science for Life Laboratory, Department of Protein Science, KTH Royal Institute of Technology, 114 17 Stockholm, Sweden
Jacquiod, Samuel; Agroécologie, AgroSup Dijon, INRAE, Université de Bourgogne Franche-Comté, F-21000 Dijon, France
Sørensen, Søren J; Section of Microbiology, Department of Biology, University of Copenhagen, 2100 Copenhagen, Denmark
Ledesma-Amaro, Rodrigo ; Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK ; Department of Bioengineering, Imperial College London, London SW7 2AZ, UK
Language :
English
Title :
Comparative Genomics Analysis of Keratin-Degrading Chryseobacterium Species Reveals Their Keratinolytic Potential for Secondary Metabolite Production.
This research was funded by the Innovation Fund Denmark (Grant Number 1308-00015B, Keratin2Protein) and also under the support of the Chinese Scholarship Council Program. R.L.-A. received funding from BBSRC (BB/R01602X/1), 19-ERACoBioTech-33 SyCoLim BB/T011408/1, BBSRC BB/T013176/1, British Council 527429894, European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (DEUSBIO-949080). S.S. was supported by Engineering and Physical Sciences Research Council (EPSRC) (EP/S001301/1), Biotechnology Biological Sciences Research Council (BBSRC) (BB/S016899/1), and Science for Life Laboratory (SciLifeLab).
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