Bioscreening and pre-clinical evaluation of the impact of bioactive molecules from Ptychotis verticillata on the multilineage potential of mesenchymal stromal cells towards immune- and inflammation-mediated diseases.
[en] OBJECTIVE AND DESIGN: Mesenchymal stromal cells (MSCs) are currently used in cell reparative medicine due to their trophic and ant-inflammatory properties. The modulation of stem cell properties by phytochemicals has been suggested as a tool to empower their tissue repair capacity. In vitro, MSCs are characterized by their tri-lineage potential that holds great interest for tissue regeneration. Ptychotis Verticillata (PV), an aromatic and medicinal plant, may be thus used to modulate the in vitro multilineage potential of MSCs.
MATERIALS AND METHODS: We screened the impact of PV-derived essential oil and their bioactive molecules (thymol and carvacrol) on the in vitro multilineage potential of MSCs. Different concentrations and incubation times of these compounds were assessed during the osteogenesis and adipogenesis of MSCs.
RESULTS: The analysis of 75 conditions indicates that these compounds are biologically active by promoting two major differentiation lineages from MSCs. In a time- and dose-dependent manner, thymol and carvacrol increased the osteogenesis and adipogenesis.
CONCLUSION: According to these preliminary observations, the addition of PV extract may stimulate the tissue regenerative and repair functions of MSCs. Further optimization of compound extraction and characterization from PV as well as cell treatment conditions should increase their therapeutic value in combination with MSCs.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Bouhtit, Fatima; Laboratory of Experimental Hematology, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium ; Genetics and Immune Cell Therapy Unit, Faculty of Sciences, University Mohammed Premier, Oujda, Morocco
Najar, Mehdi ; Laboratory of Clinical Cell Therapy, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium. mnajar@ulb.ac.be ; Osteoarthritis Research Unit, University of Montreal Hospital Research Center (CRCHUM), Montreal, Canada. mnajar@ulb.ac.be ; Department of Medicine, University of Montreal, Montreal, Canada. mnajar@ulb.ac.be
Rahmani, Saida; Laboratory of Experimental Hematology, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium ; Genetics and Immune Cell Therapy Unit, Faculty of Sciences, University Mohammed Premier, Oujda, Morocco
Melki, Rahma; Genetics and Immune Cell Therapy Unit, Faculty of Sciences, University Mohammed Premier, Oujda, Morocco
Najimi, Mustapha; Institut de Recherche Expérimentale et Clinique (IREC), Laboratory of Pediatric Hepatology and Cell Therapy, Université Catholique de Louvain, Brussels, Belgium
Sadki, Khalid; Laboratory of Human Pathologies Biology, Faculty of Sciences, Mohammed V University Rabat, Agdal, Rabat, Morocco
Boukhatem, Noreddine; Genetics and Immune Cell Therapy Unit, Faculty of Sciences, University Mohammed Premier, Oujda, Morocco
Twizere, Jean-Claude ; Université de Liège - ULiège > TERRA Research Centre > Microbial technologies ; Center for Cancer Systems Biology (CCSB), Dana-Farber Cancer Institute, Boston, MA, USA
Meuleman, Nathalie; Laboratory of Experimental Hematology, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
Lewalle, Philippe; Laboratory of Experimental Hematology, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
Lagneaux, Laurence; Laboratory of Clinical Cell Therapy, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
Merimi, Makram; Laboratory of Experimental Hematology, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium ; Genetics and Immune Cell Therapy Unit, Faculty of Sciences, University Mohammed Premier, Oujda, Morocco
Language :
English
Title :
Bioscreening and pre-clinical evaluation of the impact of bioactive molecules from Ptychotis verticillata on the multilineage potential of mesenchymal stromal cells towards immune- and inflammation-mediated diseases.
Publication date :
August 2022
Journal title :
Inflammation Research
ISSN :
1023-3830
eISSN :
1420-908X
Publisher :
Springer Science and Business Media Deutschland GmbH, Switzerland
F.R.S.-FNRS - Fonds de la Recherche Scientifique Télévie
Funding text :
We would like to thank the Cell Therapy Unit team for their helpful discussions and Karlien Pieters for her technical assistance. This study has received support from the Generation Life Foundation, “Fonds National de la Recherche Scientifique (FNRS),” “Télévie,” “Les Amis de l’Institut Jules Bordet”, the Research Project (PDR).
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