Conservation genetics; Muscardinus avellanarius; nGBS; Non-invasive monitoring; SNP discovery; Ecology, Evolution, Behavior and Systematics; Genetics
Abstract :
[en] The hazel dormouse Muscardinus avellanarius presents an exemplary non-model species that is both locally threatened and whose genetic status is not fully understood owing to insufficient resolution of the currently available molecular tools. We performed normalized Genotyping-by-Sequencing (nGBS) on 48 hazel dormouse samples collected across the species European distribution, aiming at discovering useful single nucleotide polymorphism (SNP) markers for the assessment of population structure and genomic diversity. The analyses of > 24,000 SNPs showed a high divergence between the Eastern and Western lineage of the species with high rates of SNP allele fixation, consistent with previous studies suggesting the divergence of lineages occurred over 2 mya. These results indicate that investigating inter-lineage as well as within-lineage genetic composition will be a conclusive approach for identifying conservation strategies in the future. Results presented here indicate the highest genetic divergence in the Italian and Lithuanian populations. We document how nGBS allows the discovery of SNPs that can characterize patterns of genetic variation at multiple spatial scales in a non-model organism. We document how nGBS allows the discovery of SNPs that can characterize patterns of genetic variation at multiple spatial scales in a non-model organism, potentially informing monitoring and conservation strategies.
Disciplines :
Zoology
Author, co-author :
Leyhausen, Johanna ; Conservation Genetics Group, Senckenberg Research Institute and Natural History Museum Frankfurt, Gelnhausen, Germany
Cocchiararo, Berardino; Conservation Genetics Group, Senckenberg Research Institute and Natural History Museum Frankfurt, Gelnhausen, Germany ; LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Frankfurt am Main, Germany
Nowak, Carsten; Conservation Genetics Group, Senckenberg Research Institute and Natural History Museum Frankfurt, Gelnhausen, Germany ; LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Frankfurt am Main, Germany
Ansorge, Hermann; Senckenberg Museum of Natural History Görlitz, Görlitz, Germany
Bertolino, Sandro ; Department of Life Sciences and Systems Biology, University of Turin, Torino, Italy
Büchner, Sven; Senckenberg Museum of Natural History Görlitz, Görlitz, Germany
Fietz, Joanna ; Department of Zoology, Institute of Biology, Stuttgart, Germany
Foppen, Ruud ; Department of Animal Ecology and Ecophysiology, Radboud Institute for Biological and Environmental Sciences, Radboud University Nijmegen/Dutch Mammal Society, Nijmegen, Netherlands
Juškaitis, Rimvydas ; Nature Research Centre, Vilnius, Lithuania
La Haye, Maurice ; Department of Animal Ecology and Ecophysiology, Radboud Institute for Biological and Environmental Sciences, Radboud University Nijmegen/Dutch Mammal Society, Nijmegen, Netherlands
Lang, Johannes ; Working Group for Wildlife Research, Clinic for Birds, Reptiles, Amphibians and Fish, Justus-Liebig-University Giessen, Giessen, Germany
Michaux, Johan ; Université de Liège - ULiège > Département des sciences de la vie > Laboratoire de génétique de la conservation
Verbeylen, Goedele ; Natuurpunt Studie (Mammal Working Group), Mechelen, Belgium
von Thaden, Alina ; Conservation Genetics Group, Senckenberg Research Institute and Natural History Museum Frankfurt, Gelnhausen, Germany ; LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Frankfurt am Main, Germany
Mueller, Sarah A. ; Conservation Genetics Group, Senckenberg Research Institute and Natural History Museum Frankfurt, Gelnhausen, Germany ; LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Frankfurt am Main, Germany
This study was partially funded by the Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz of the German Federal State of Hesse (LOEWE). Part of funding for sample collection came from the DFG Grant DFG: FI 831/6-1 (Joanna Fietz).
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