[en] Dentoalveolar tissue engineering is an emerging yet challenging field, considering the lack of suitable materials and difficulty to produce patient-specific hydrogel scaffolds. The present paper aims to produce a 3D printable and tuneable biomaterial by copolymerizing a synthesized water-soluble chitosan derivative called maleic anhydride grafted chitosan (MA-C) with gelatin using genipin, a natural crosslinking agent. Development and testing of this material for 3D printing, degradation, and swelling demonstrated the ability to fabricate scaffolds with controlled physical properties based on pre-determined designs. The MA-C-gelatin copolymer demonstrated excellent biocompatibility, which was verified by analyzing the viability, growth and proliferation of human dental pulp stem cells seeded on MA-C-gelatin constructs through live/dead, alamar blue and DNA quantification assays. Based on the present findings, the proposed material might be a suitable candidate for dentoalveolar tissue engineering, while further research is required to achieve this goal.
Disciplines :
Engineering, computing & technology: Multidisciplinary, general & others
Author, co-author :
Salar Amoli, Mehdi; Surface and Interface Engineered Materials (SIEM), Campus Group T, KU Leuven, Andreas Vesaliusstraat 13, 3000 Leuven, Belgium, OMFS IMPATH Research Group, Faculty of Medicine, Department of Imaging and Pathology, KU Leuven and Oral and Maxillofacial Surgery, University Hospitals Leuven, Kapucijnenvoer 33, 3000 Leuven, Belgium. Electronic address: mehdi.salaramoli@kuleuven.be
Anand, Resmi; Surface and Interface Engineered Materials (SIEM), Campus Group T, KU Leuven, Andreas Vesaliusstraat 13, 3000 Leuven, Belgium, Prometheus, Division of Skeletal Tissue Engineering Leuven, KU Leuven, Leuven, Belgium, Inter University Centre for Biomedical Research and Super Speciality Hospital, Mahatma Gandhi University Campus at Thalappady, Kottayam, Kerala 686009, India. Electronic address: resmi.anand@list.lu
EzEldeen, Mostafa; OMFS IMPATH Research Group, Faculty of Medicine, Department of Imaging and Pathology, KU Leuven and Oral and Maxillofacial Surgery, University Hospitals Leuven, Kapucijnenvoer 33, 3000 Leuven, Belgium, Department of Oral Health Sciences, KU Leuven and Paediatric Dentistry and Special Dental Care, University Hospitals Leuven, Kapucijnenvoer 33, 3000 Leuven, Belgium. Electronic address: mostafa.ezeldeen@kuleuven.be
Amorim, Paulo Alexandre; Surface and Interface Engineered Materials (SIEM), Campus Group T, KU Leuven, Andreas Vesaliusstraat 13, 3000 Leuven, Belgium, Prometheus, Division of Skeletal Tissue Engineering Leuven, KU Leuven, Leuven, Belgium. Electronic address: pauloalexandre.amorim@kuleuven.be
Geris, Liesbet ; Université de Liège - ULiège > GIGA > GIGA In silico medecine - Biomechanics Research Unit
Jacobs, Reinhilde; OMFS IMPATH Research Group, Faculty of Medicine, Department of Imaging and Pathology, KU Leuven and Oral and Maxillofacial Surgery, University Hospitals Leuven, Kapucijnenvoer 33, 3000 Leuven, Belgium, Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden. Electronic address: reinhilde.jacobs@kuleuven.be
Bloemen, Veerle; Surface and Interface Engineered Materials (SIEM), Campus Group T, KU Leuven, Andreas Vesaliusstraat 13, 3000 Leuven, Belgium, Prometheus, Division of Skeletal Tissue Engineering Leuven, KU Leuven, Leuven, Belgium. Electronic address: veerle.bloemen@kuleuven.be
Language :
English
Title :
The development of a 3D printable chitosan-based copolymer with tunable properties for dentoalveolar regeneration.
ERC - European Research Council KU Leuven - Katholieke Universiteit Leuven
Funding text :
Supported by the Research Council of KU Leuven grant number ( C24/18/068 ), and the European Research Council (ERC) under the European Union 's Horizon 2020 research and innovation programme ( ERC CoG 772418 ). RA acknowledges DST INSPIRE Faculty award ( DST/INSPIRE/04/2016/000482 ).
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