[en] Methacryloyl gelatin (GelMA) is a versatile material for bioprinting because of its tunable physical properties and inherent bioactivity. Bioprinting of GelMA is often met with challenges such as lower viscosity of GelMA inks due to higher methacryloyl substitution and longer physical gelation time at room temperature. In this study, a tunable interpenetrating polymer network (IPN) hydrogel was prepared from gelatin-hyaluronan dialdehyde (Gel-HDA) Schiff's polymer, and 100% methacrylamide substituted GelMA for biofabrication through extrusion based bioprinting. Temperature sweep rheology measurements show a higher sol-gel transition temperature for IPN (30 °C) compared to gold standard GelMA (27 °C). Furthermore, to determine the tunability of the IPN hydrogel, several IPN samples were prepared by combining different ratios of Gel-HDA and GelMA achieving a compressive modulus ranging from 20.6 ± 2.48 KPa to 116.7 ± 14.80 KPa. Our results showed that the mechanical properties and printability at room temperature could be tuned by adjusting the ratios of GelMA and Gel-HDA. To evaluate cell response to the material, MC3T3-E1 mouse pre-osteoblast cells were embedded in hydrogels and 3D-printed, demonstrating excellent cell viability and proliferation after 10 d of 3Din vitroculture, making the IPN an interesting bioink for the fabrication of 3D constructs for tissue engineering applications.
Disciplines :
Engineering, computing & technology: Multidisciplinary, general & others
Author, co-author :
Anand, Resmi ; Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, O&N 1, Herestraat 49, 3000 Leuven, Belgium ; Surface and Interface Engineered Materials (SIEM), Campus Group T, KU Leuven, Andreas Vesaliusstraat 13, 3000 Leuven, Belgium ; Inter University Centre for Biomedical Research and Super Speciality Hospital, Mahatma Gandhi University Campus at Thalappady, Kottayam, Kerala 686009, India
Salar Amoli, Mehdi; Surface and Interface Engineered Materials (SIEM), Campus Group T, KU Leuven, Andreas Vesaliusstraat 13, 3000 Leuven, Belgium ; Department of Imaging & Pathology/OMFS-IMPATH Research Group, Campus Sint-Rafaël, KU Leuven, Kapucijnenvoer 33, 3000 Leuven, Belgium
Huysecom, An-Sofie; Soft Matter, Rheology and Technology, Department of Chemical Engineering, KU Leuven, Leuven, Belgium
Amorim, Paulo Alexandre; Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, O&N 1, Herestraat 49, 3000 Leuven, Belgium ; Surface and Interface Engineered Materials (SIEM), Campus Group T, KU Leuven, Andreas Vesaliusstraat 13, 3000 Leuven, Belgium
Agten, Hannah; Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, O&N 1, Herestraat 49, 3000 Leuven, Belgium ; Surface and Interface Engineered Materials (SIEM), Campus Group T, KU Leuven, Andreas Vesaliusstraat 13, 3000 Leuven, Belgium
Geris, Liesbet ; Université de Liège - ULiège > GIGA > GIGA In silico medecine - Biomechanics Research Unit ; Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, O&N 1, Herestraat 49, 3000 Leuven, Belgium ; Biomechanics Section, KU Leuven, Celestijnenlaan 300C (2419), Leuven, Belgium
Bloemen, Veerle; Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, O&N 1, Herestraat 49, 3000 Leuven, Belgium ; Surface and Interface Engineered Materials (SIEM), Campus Group T, KU Leuven, Andreas Vesaliusstraat 13, 3000 Leuven, Belgium
Language :
English
Title :
A tunable gelatin-hyaluronan dialdehyde/methacryloyl gelatin interpenetrating polymer network hydrogel for additive tissue manufacturing.
H2020 - 772418 - INSITE - Development and use of an integrated in silico-in vitro mesofluidics system for tissue engineering
Funders :
ERC - European Research Council KU Leuven - Catholic University of Leuven EU - European Union
Funding number :
DST/ INSPIRE/04/2016/000482
Funding text :
The authors acknowledge funding from European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation
programme (Grant Agreement No. 772418). R A acknowledges DST INSPIRE Faculty award (DST/ INSPIRE/04/2016/000482) for financial support.
M S A acknowledges Research Council of KU Leuven Grant No. (C24/18/068).
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