Complementary and alternative medicine; Pharmacology
Abstract :
[en] Objectives: The chemical composition of cactus pear seed oil (Opuntia ficus-indica [L.]
Mill.) was analyzed in terms of its fatty acid composition, tocopherol content, phenolic
identification, and the oil’s phenolic-rich fraction antioxidant power was determined.
Methods: Fatty acid profiling was performed by gas chromatography coupled to an FI detector. Tocopherols and phenolic compounds were analyzed by LC-FLD/UV, and the oil’s
phenolic-rich fraction antioxidant power was determined by phosphomolybdenum, DPPH
assay and β-carotene bleaching test.
Results: Fatty acid composition was marked by a high unsaturation level (83.22 ± 0.34%).
The predominant fatty acid was linoleic acid (66.79 ± 0.78%), followed by oleic acid
(15.16 ± 0.42%) and palmitic acid (12.70 ± 0.03%). The main tocopherol was γ-tocopherol
(172.59 ± 7.59 mg/kg. In addition, Tyrosol, vanillic acid, vanillin, ferulic acid, pinoresinol,
and cinnamic acid were identified as phenolic compounds in the analyzed seed oil.
Moreover, the oil’s phenolics-rich fraction showed a significant total antioxidant activity,
scavenged DPPH up to 97.85%, and effectively protected β-carotene against bleaching
(97.56%).
Conclusion: The results support the potential use of cactus pear seed oil as a functional
food.
Disciplines :
Agriculture & agronomy Chemistry
Author, co-author :
Ali, Berraaouan ; Laboratory of Bioressources, Biotechnologies, Ethnopharmacology and Health, Mohammed the First University, Faculty of Sciences, Oujda, Morocco
Abderrahim, Ziyyat ; Laboratory of Bioressources, Biotechnologies, Ethnopharmacology and Health, Mohammed the First University, Faculty of Sciences, Oujda, Morocco
Hassane, Mekhfi ; Laboratory of Bioressources, Biotechnologies, Ethnopharmacology and Health, Mohammed the First University, Faculty of Sciences, Oujda, Morocco
Abdelkhaleq, Legssyer ; Laboratory of Bioressources, Biotechnologies, Ethnopharmacology and Health, Mohammed the First University, Faculty of Sciences, Oujda, Morocco
Mohammed, Aziz ; Laboratory of Bioressources, Biotechnologies, Ethnopharmacology and Health, Mohammed the First University, Faculty of Sciences, Oujda, Morocco
Mohamed, Bnouham ; Laboratory of Bioressources, Biotechnologies, Ethnopharmacology and Health, Mohammed the First University, Faculty of Sciences, Oujda, Morocco
Language :
English
Title :
Chemical Composition of Cactus Pear Seed Oil: phenolics identification and antioxidant activity
El Mannoubi I, Barrek S, Skanji T, Casabianca H, Zarrouk H. Characterization of Opuntia ficus indica seed oil from Tunisia. Chem Nat Compd. 2009;45(5):616-20.
Tounsi MS, Ouerghemmi I, Ksouri R, Wannes WA, Hammrouni I, Marzouk B. HPLC-determination of phenolic composition and antioxidant capacity of cactus prickly pears seeds. Asian J Chem. 2011;23(3):1006-10.
Chougui N, Tamendjari A, Hamidj W, Hallal S, Barras A, Richard T, et al. Oil composition and characterisation of phenolic compounds of Opuntia ficus-indica seeds. Food Chem. 2013;139(1-4):796-803.
Nounah I, Chbani M, Matthaus B, Charrouf Z, Hajib A, Willenberg I. Profile of volatile aroma-active compounds of cactus seed oil (Opuntia ficus-indica) from different locations in Morocco and their fate during seed roasting. Foods. 2020;9(9):1280.
Moumen AB, Mansouri F, Richard G, Abid M, Fauconnier ML, Sindic M, et al. Biochemical characterisation of the seed oils of four safflower (Carthamus tinctorius) varieties grown in northeastern of Morocco. Int J Food Sci Technol. 2015;50(3):804-10.
Brenes M, García A, García P, Garrido A. Rapid and complete extraction of phenols from olive oil and determination by means of a coulometric electrode array system. J Agric Food Chem. 2000;48(11):5178-83.
Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat Protoc. 2007;2(4):875-7.
Kumaran A, Joel Karunakaran R. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT. 2007;40(2):344-52.
Liu W, Fu YJ, Zu YG, Tong MH, Wu N, Liu XL, et al. Supercritical carbon dioxide extraction of seed oil from Opuntia dillenii Haw. and its antioxidant activity. Food Chem. 2009;114(1):334-9.
Sahreen S, Khan MR, Khan RA. Evaluation of antioxidant activities of various solvent extracts of Carissa opaca fruits. Food Chem. 2010;122(4):1205-11.
Ramadan M, Mörsel JT. Direct isocratic normal-phase HPLC assay of fat-soluble vitamins and β-carotene in oilseeds. Eur Food Res Technol. 2002;214(6):521-7.
Labuschagne MT, Hugo A. Oil content and fatty acid composition of cactus pear seed compared with cotton and grape seed. J Food Biochem. 2010;34(1):93-100.
Coşkuner Yn, Tekin A. Monitoring of seed composition of prickly pear (Opuntia ficus-indica L) fruits during maturation period. J Sci Food Agric. 2003;83(8):846-9.
Al-Khudairy L, Hartley L, Clar C, Flowers N, Hooper L, Rees K. Omega 6 fatty acids for the primary prevention of cardiovascular disease. Cochrane Database Syst Rev. 2015;(11):CD011094.
Martins JLR, Silva DM, Gomes EH, Fava SA, Carvalho MF, Macedo IYL, et al. Evaluation of gastroprotective activity of linoleic acid on gastric ulcer in a mice model. Curr Pharm Des. 2022;28(8):655-60.
Sales-Campos H, Souza PR, Peghini BC, da Silva JS, Cardoso CR. An overview of the modulatory effects of oleic acid in health and disease. Mini Rev Med Chem. 2013;13(2):201-10.
Matthäus B, Özcan MM. Habitat effects on yield, fatty acid composition and tocopherol contents of prickly pear (Opuntia ficus-indica L.) seed oils. Sci Hortic. 2011;131:95-8.
Cao J, Li H, Xia X, Zou XG, Li J, Zhu XM, et al. Effect of fatty acid and tocopherol on oxidative stability of vegetable oils with limited air. Int J Food Prop. 2015;18(4):808-20.
Kamal-Eldin A, Andersson R. A multivariate study of the correlation between tocopherol content and fatty acid composition in vegetable oils. J Am Oil Chem Soc. 1997;74(4):375-80.
Pryor WA. TOCOPHEROLS/Physiology. In: Caballero B, editor. Encyclopedia of food sciences and nutrition. 2nd ed: Amsterdam: Academic Press; 2003. p. 5796-800.
Jiang Q, Ames BN. Gamma-tocopherol, but not alpha-tocopherol, decreases proinflammatory eicosanoids and inflammation damage in rats. FASEB J. 2003;17(8):816-22.
Tlili N, Bargougui A, Elfalleh W, Triki S, Nasri N. Phenolic compounds, protein, lipid content and fatty acids compositions of cactus seeds. J Med Plants Res. 2011;5(18):4519-24.
Haig T. Allelochemicals in plants. In: Zeng R, Mallik A, Luo S, editors. Allelopathy in sustainable agriculture and forestry. New York (NY): Springer; 2008. p. 63-104.
Inderjit. Plant phenolics in allelopathy. Bot Rev. 1996;62(2):186-202.
Dziedzic SZ, Hudson BJF. Phenolic acids and related compounds as antioxidants for edible oils. Food Chem. 1984;14(1):45-51.
Wanasundara PKJPD, Shahidi F, Shukla VKS. Endogenous antioxidants from oilseeds and edible oils. Food Rev Int. 1997;13(2):225-92.
Kamat JP, Ghosh A, Devasagayam TP. Vanillin as an antioxidant in rat liver mitochondria: inhibition of protein oxidation and lipid peroxidation induced by photosensitization. Mol Cell Biochem. 2000;209(1-2):47-53.
Lirdprapamongkol K, Sakurai H, Kawasaki N, Choo MK, Saitoh Y, Aozuka Y, et al. Vanillin suppresses in vitro invasion and in vivo metastasis of mouse breast cancer cells. Eur J Pharm Sci. 2005;25(1):57-65.
Lee J, Cho JY, Lee SY, Lee KW, Lee J, Song JY. Vanillin protects human keratinocyte stem cells against ultraviolet B irradiation. Food Chem Toxicol. 2014;63:30-7.
Bu Y, Rho S, Kim J, Kim MY, Lee DH, Kim SY, et al. Neuroprotective effect of tyrosol on transient focal cerebral ischemia in rats. Neurosci Lett. 2007;414(3):218-21.
Dewapriya P, Himaya SW, Li YX, Kim SK. Tyrosol exerts a protective effect against dopaminergic neuronal cell death in in vitro model of Parkinson’s disease. Food Chem. 2013;141(2):1147-57.
Ou S, Kwok KC. Ferulic acid: pharmaceutical functions, preparation and applications in foods. J Sci Food Agric. 2004;84(11): 1261-9.
Tai A, Sawano T, Ito H. Antioxidative properties of vanillic acid esters in multiple antioxidant assays. Biosci Biotechnol Biochem. 2012;76(2):314-8.
Kumar S, Prahalathan P, Raja B. Vanillic acid: a potential inhibitor of cardiac and aortic wall remodeling in l-NAME induced hypertension through upregulation of endothelial nitric oxide synthase. Environ Toxicol Pharmacol. 2014;38(2):643-52.
Shen SC, Chang WC, Chen JW, Kuo PL, Wu JSB. Anti-hyperglycemic effect of vanillic acid in high-fat diet induced prediabetic rats. Ann Nutr Metab. 2013;63:1181.
Akao Y, Maruyama H, Matsumoto K, Ohguchi K, Nishizawa K, Sakamoto T, et al. Cell growth inhibitory effect of cinnamic acid derivatives from propolis on human tumor cell lines. Biol Pharm Bull. 2003;26(7):1057-9.
Cinkilic N, Tüzün E, Çetintaş SK, Vatan Ö, Yılmaz D, Çavaş T, et al. Radio-protective effect of cinnamic acid, a phenolic phytochemical, on genomic instability induced by X-rays in human blood lymphocytes in vitro. Mutat Res Genet Toxicol Environ Mutagen. 2014;770:72-9.
Antolovich M, Prenzler PD, Patsalides E, McDonald S, Robards K. Methods for testing antioxidant activity. Analyst. 2002;127(1):183-98.
Koleva II, van Beek TA, Linssen JP, de Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal. 2002;13(1):8-17.
Grigelmo-Miguel N, Rojas-Graü MA, Soliva-Fortuny R, Martín-Belloso O. Methods of analysis of antioxidant capacity of phytochemicals. In: de la Rosa LA, Alvarez-Parrilla E, González-Aguilar GA, editors. Fruit and vegetable phytochemicals: chemistry and human health. 2nd ed. Chichester: Wiley-Blackwell; 2009. p. 271-307.
Del Rio D, Rodriguez-Mateos A, Spencer JP, Tognolini M, Borges G, Crozier A. Dietary (poly)phenolics in human health: structures, bioavailability, and evidence of protective effects against chronic diseases. Antioxid Redox Signal. 2013;18(14):1818-92.
Benayad Z, Martinez-Villaluenga C, Frias J, Gomez-Cordoves C, Es-Safi NE. Phenolic composition, antioxidant and anti-inflammatory activities of extracts from Moroccan Opuntia ficus-indica flowers obtained by different extraction methods. Ind Crops Prod. 2014;62:412-20.
