SARS-CoV-2; adenovirus; blue LED light; respiratory syncytial virus; viral inactivation; Immunology and Allergy; Molecular Biology; Immunology and Microbiology (all); Microbiology (medical); Infectious Diseases; General Immunology and Microbiology
Abstract :
[en] Blue LED light has proven to have a powerful bacteria-killing ability; however, little is known about its mechanism of virucidal activity. Therefore, we analyzed the effect of blue light on different respiratory viruses, such as adenovirus, respiratory syncytial virus and SARS-CoV-2. The exposure of samples to a blue LED light with a wavelength of 420 nm (i.e., in the visible range) at 20 mW/cm2 of irradiance for 15 min appeared optimal and resulted in the complete inactivation of the viral load. These results were similar for all the three viruses, demonstrating that both enveloped and naked viruses could be efficiently inactivated with blue LED light, regardless of the presence of envelope and of the viral genome nature (DNA or RNA). Moreover, we provided some explanations to the mechanisms by which the blue LED light could exert its antiviral activity. The development of such safe and low-cost light-based devices appears to be of fundamental utility for limiting viral spread and for sanitizing small environments, objects and surfaces, especially in the pandemic era.
Disciplines :
Microbiology Immunology & infectious disease
Author, co-author :
Terrosi, Chiara; Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy
Anichini, Gabriele ; Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy
Docquier, Jean-Denis ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'Ingénierie des Protéines (CIP)
Gori Savellini, Gianni ; Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy
Gandolfo, Claudia ; Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy
Pavone, Francesco Saverio; Department of Physics and Astronomy, European Laboratory for Non Linear Spectroscopy (LENS), University of Florence, 50121 Florence, Italy
Cusi, Maria Grazia ; Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy
Language :
English
Title :
Efficient Inactivation of SARS-CoV-2 and Other RNA or DNA Viruses with Blue LED Light.
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