Article (Scientific journals)
Automated Open-Hardware Multiwell Imaging Station for Microorganisms Observation
Gervasi, Alain; Cardol, Pierre; Meyer, Patrick
2022In Micromachines, 13 (6), p. 833
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Keywords :
Electrical and Electronic Engineering; Mechanical Engineering; Control and Systems Engineering
Abstract :
[en] Bright field microscopes are particularly useful tools for biologists for cell and tissue observation, phenotyping, cell counting, and so on. Direct cell observation provides a wealth of information on cells’ nature and physiological condition. Microscopic analyses are, however, time-consuming and usually not easy to parallelize. We describe the fabrication of a stand-alone microscope able to automatically collect samples with 3D printed pumps, and capture images at up to 50× optical magnification with a digital camera at a good throughput (up to 24 different samples can be collected and scanned in less than 10 min). Furthermore, the proposed device can store and analyze pictures using computer vision algorithms running on a low power integrated single board computer. Our device can perform a large set of tasks, with minimal human intervention, that no single commercially available machine can perform. The proposed open-hardware device has a modular design and can be freely reproduced at a very competitive price with the use of widely documented and user-friendly components such as Arduino, Raspberry pi, and 3D printers.
Research Center/Unit :
InBios - Integrative Biological Sciences - ULiège
Disciplines :
Biotechnology
Author, co-author :
Gervasi, Alain  ;  Université de Liège - ULiège > Faculté des Sciences > Master bioinf. & mod., à fin.
Cardol, Pierre  ;  Université de Liège - ULiège > Integrative Biological Sciences (InBioS)
Meyer, Patrick  ;  Université de Liège - ULiège > Integrative Biological Sciences (InBioS)
Language :
English
Title :
Automated Open-Hardware Multiwell Imaging Station for Microorganisms Observation
Publication date :
26 May 2022
Journal title :
Micromachines
ISSN :
2072-666X
Publisher :
MDPI AG
Volume :
13
Issue :
6
Pages :
833
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique
Funding number :
PDR T.0032
Available on ORBi :
since 31 May 2022

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