[en] Helminth infection conditions lung macrophages in the long term, but little is known about how helminths affect the lung macrophage responses to respiratory viral coinfection. While comparing BALB/c and C57BL/6 mice, we found that helminth infection in C57BL/6 mice resulted in enhanced permissiveness to a subsequent infection with murid gammaherpesvirus 4 (MuHV-4), and that viral early tropism was mainly restricted to lung macrophages. Helminth infection resulted in enhanced type 2 airway inflammation and M(IL-4) polarization of interstitial macrophages (IntMs) in C57BL/6 mice, associated with an IL-4Rα-dependent disappearance reaction of alveolar macrophages (AlvMs) and enriched monocyte-derived IntMs proportions. Competent IL-4Rα responsiveness or intra-tracheal instillation of recombinant IL-4 or IL-13 significantly resulted in reduced numbers of AlvMs and enriched IntMs as well as increased permissiveness to MuHV-4 infection, which was restricted to AlvMs. Thus, direct IL-4Rα signaling during helminth infection affects macrophage permissiveness to gammaherpesvirus infection.
Disciplines :
Immunology & infectious disease Veterinary medicine & animal health
Author, co-author :
Petrellis, Georgios ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Rolot, Marion ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Preure, Amira ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH)
Wathieu, Caroline ; Université de Liège - ULiège > GIGA > GIGA Stem Cells - Medical Chemistry
Dougall, Annette ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Immunologie vétérinaire
Chetty, Alisha; Wellcome Centre for Infectious Diseases Research in Africa (CIDRI-Africa) > Department of Pathology, Division of Immunology > Institute of Infectious Disease and Molecular Medicine (IDM)
CHARIOT, Alain ; Université de Liège - ULiège > GIGA > GIGA Stem Cells - Medical Chemistry
Hornsell, William; Wellcome Centre for Infectious Diseases Research in Africa (CIDRI-Africa) > Department of Pathology, Division of Immunology > Institute of Infectious Disease and Molecular Medicine (IDM)
Dewals, Benjamin G ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Immunologie vétérinaire
Language :
English
Title :
IL-4rα-dependent Lung Macrophage Responses To Helminths Increase Permissiveness To Gammaherpesvirus Infection
Publication date :
09 May 2022
Event name :
Type 2 Immunity in Early Life
Event place :
Liège, Belgium
Event date :
9 & 10 May 2022
Audience :
International
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique ULiège - Université de Liège