Article (Scientific journals)
Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production.
Bustos Cosios, Cristina Veronica; Quezada, Johan; Veas, Rhonda et al.
2022In Metabolites, 12 (4), p. 346
Peer Reviewed verified by ORBi
 

Files


Full Text
Metabolites_2022.pdf
Author postprint (984.73 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Komagataella phaffii; Pichia pastoris; cell engineering; metabolic engineering; recombinant protein; Endocrinology, Diabetes and Metabolism; Biochemistry; Molecular Biology
Abstract :
[en] Komagataella phaffii (formerly known as Pichia pastoris) has become an increasingly important microorganism for recombinant protein production. This yeast species has gained high interest in an industrial setting for the production of a wide range of proteins, including enzymes and biopharmaceuticals. During the last decades, relevant bioprocess progress has been achieved in order to increase recombinant protein productivity and to reduce production costs. More recently, the improvement of cell features and performance has also been considered for this aim, and promising strategies with a direct and substantial impact on protein productivity have been reported. In this review, cell engineering approaches including metabolic engineering and energy supply, transcription factor modulation, and manipulation of routes involved in folding and secretion of recombinant protein are discussed. A lack of studies performed at the higher-scale bioreactor involving optimisation of cultivation parameters is also evidenced, which highlights new research aims to be considered.
Disciplines :
Biotechnology
Author, co-author :
Bustos Cosios, Cristina Veronica  ;  Université de Liège - ULiège > TERRA Research Centre ; School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso 2362803, Chile
Quezada, Johan ;  School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso 2362803, Chile
Veas, Rhonda;  School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso 2362803, Chile
Altamirano, Claudia;  School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso 2362803, Chile
Braun-Galleani, Stephanie ;  School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso 2362803, Chile
Fickers, Patrick  ;  Université de Liège - ULiège > Département GxABT > Microbial technologies
Berrios, Julio ;  School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso 2362803, Chile
Language :
English
Title :
Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production.
Publication date :
14 April 2022
Journal title :
Metabolites
eISSN :
2218-1989
Publisher :
MDPI, Switzerland
Volume :
12
Issue :
4
Pages :
346
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FONDECYT - Chile Fondo Nacional de Desarrollo Científico y Tecnológico [CL]
WBI - Wallonie-Bruxelles International [BE]
Funding text :
Funding: This research was funded by FONDECYT Regular grant number 1191196, FONDECYT Ini-ciación grant number 11200933, ANILLO Regular de Ciencia y Tecnología grant number ACT210068, and Becas Doctorado Nacional grant numbers 21191422, 21211138, 21211233—Agencia Nacional de Investigación y Desarrollo (ANID), Chile; and Wallonie-Bruxelles International through the Cooperation bilateral Belgique-Chili project SUB/2019/435787.This research was funded by FONDECYT Regular grant number 1191196, FONDECYT Iniciaci?n grant number 11200933, ANILLO Regular de Ciencia y Tecnolog?a grant number ACT210068, and Becas Doctorado Nacional grant numbers 21191422, 21211138, 21211233?Agencia Nacional de Investigaci?n y Desarrollo (ANID), Chile; and Wallonie-Bruxelles International through the Cooperation bilateral Belgique-Chili project SUB/2019/435787.
Available on ORBi :
since 16 May 2022

Statistics


Number of views
43 (2 by ULiège)
Number of downloads
83 (2 by ULiège)

Scopus citations®
 
15
Scopus citations®
without self-citations
14
OpenCitations
 
2

Bibliography


Similar publications



Contact ORBi