Article (Scientific journals)
Inflammatory profile of induced sputum composition in systemic sclerosis and comparison with healthy volunteers.
JACQUERIE, Pierre; HENKET, Monique; André, B et al.
2021In Scientific Reports, 11 (1), p. 10679
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Keywords :
Biomarkers; Cytokines; Inflammation Mediators; Cytokines/metabolism; Healthy Volunteers; Humans; Inflammation Mediators/blood; Inflammation Mediators/metabolism; Leukocyte Count; Leukocytes/pathology; Respiratory Function Tests; Scleroderma, Systemic/diagnosis; Scleroderma, Systemic/etiology; Scleroderma, Systemic/metabolism; Severity of Illness Index; Sputum/metabolism; Leukocytes; Scleroderma, Systemic; Sputum; Multidisciplinary
Abstract :
[en] Systemic sclerosis (SSc) is a potentially serious and disabling connective tissue disease specially in case of interstitial lung disease (SSc-ILD). The aim of our study was to evaluate the potential utility of dosing in the induced sputum (IS) and to compare their levels in SSc-ILD and SSc-nonILD patients, as well as in healthy volunteers (HV). IS and sera values were also compared. In a prospective cross-sectional analysis, we studied the IS and serum provided from 25 SSc patients, 15 SSc-nonILD and 10 SSc-ILD, compared to 25 HV. We analyzed sputum cell composition and quantified in the supernatant and corresponding serum by commercially available immunoassays: IGFBP-1, IGFBP-2, IGFBP-3, TGF-β, IL-8, TNF-α, YKL-40, MMP-7 and MMP-9. Lung function was studied by the determination of FEV-1 (%), FVC (%), DLCO (%) and KCO (%). The IS of SSc patients had a lower weight than HV (p<0.05, p<0.01) without any significant difference with regard to the cellularity. IGFBP-1 (p < 0.0001), TGF-β (p < 0.05), IL-8 (p < 0.05), YKL-40 (p < 0.0001) and MMP-7 (p < 0.01) levels were increased in the IS of SSc patients compared to HV. Only IL-8 serum levels (p < 0.001) were increased in SSc patients compared to HV. Neither in IS nor in serum were observed differences between SSc-ILD and SSc-nonILD patients. Correlations were observed between IS IL-8 levels and FEV-1 (%) (r =  = - 0.53, p < 0.01), FVC (%) (r = - 0.51, p < 0.01) and annualized ∆KCO (%) (r = 0.57, p < 0.05), between IS TGF-β levels and annualized ∆FEV-1 (%) (r =  = - 0.57, p < 0.05), between IS IGFBP-2 levels and annualized ∆KCO (%) (r = 0.56, p < 0.05). Our study showed that SSc patients exhibit raised IS levels of IGFBP-1, TGF-β, IL-8, YKL-40 and MMP-7, molecules known to be involved in lung remodeling and fibrotic process, without any significant difference between SSc-ILD and SSc-nonILD patients. IL-8, TGF-β and IGFBP-2 are correlated with lung function in SSc patients which emphasize clinical relevance. IS analysis represents a new approach to understand lung inflammatory process in SSc patients. A longitudinal study is needed to evaluate their pathophysiological relevance.
Disciplines :
Cardiovascular & respiratory systems
Rheumatology
Author, co-author :
JACQUERIE, Pierre  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie
HENKET, Monique ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de pneumologie - allergologie
André, B;  Rheumatology Department, CHU Liège, GIGA Research, ULiège, University Hospital of Liege, Domaine Universitaire du Sart-Tilman, B35, 4020, Liège, Belgium
MOERMANS, Catherine  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de pneumologie - allergologie
DE SENY, Dominique ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie
GESTER, Fanny ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de pneumologie - allergologie
LOUIS, Renaud ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de pneumologie - allergologie
MALAISE, Michel ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie
GUIOT, Julien  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de pneumologie - allergologie
Language :
English
Title :
Inflammatory profile of induced sputum composition in systemic sclerosis and comparison with healthy volunteers.
Publication date :
21 May 2021
Journal title :
Scientific Reports
eISSN :
2045-2322
Publisher :
Nature Research, England
Volume :
11
Issue :
1
Pages :
10679
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 03 May 2022

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