[en] Ionic strength strongly influenced the turnover/inactivation ratio in the interaction between beta-halogenopenicillanates and some class A beta-lactamases. This suggested the stabilization of a highly charged intermediate by solvation. Those data could be interpreted on the basis of a reaction pathway where an episulphonium ion was transiently formed. The various mechanisms proposed for explaining the formation of the dihydrothiazine chromophore are discussed.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
De Meester, F.; Université de Liège - ULiège > Laboratoires de Microbiologie et d'Enzymologie
Matagne, André ; Université de Liège - ULiège > Laboratoires de Microbiologie et d'Enzymologie
Dive, Georges ; Université de Liège - ULiège > Laboratoires de Microbiologie et d'Enzymologie
Frère, Jean-Marie ; Université de Liège - ULiège > Laboratoires de Microbiologie et d'Enzymologie
Language :
English
Title :
Unexpected Influence of Ionic Strength on Branched-Pathway Interactions between Beta-Lactamases and Beta-Halogenopenicillanates