[en] Primary graft dysfunction (PGD) is a major determinant of morbidity and mortality following lung transplantation. Delineating basic mechanisms and molecular signatures of PGD remain a fundamental challenge. This pilot study examines if the pulmonary volatile organic compound (VOC) spectrum relate to PGD and postoperative outcomes. The VOC profiles of 58 bronchoalveolar lavage fluid (BALF) and blind bronchial aspirate samples from 35 transplant patients were extracted using solid-phase-microextraction and analyzed with comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry. The support vector machine algorithm was used to identify VOCs that could differentiate patients with severe from lower grade PGD. Using 20 statistically significant VOCs from the sample headspace collected immediately after transplantation (< 6 h), severe PGD was differentiable from low PGD with an AUROC of 0.90 and an accuracy of 0.83 on test set samples. The model was somewhat effective for later time points with an AUROC of 0.80. Three major chemical classes in the model were dominated by alkylated hydrocarbons, linear hydrocarbons, and aldehydes in severe PGD samples. These VOCs may have important clinical and mechanistic implications, therefore large-scale study and potential translation to breath analysis is recommended.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Stefanuto, Pierre-Hugues ✱; Université de Liège - ULiège > Molecular Systems (MolSys) ; Thayer School of Engineering, Dartmouth College, Hanover, NH, USA
Romano, Rosalba ✱; Department of Surgery and Cancer, Section of Anaesthetics, Imperial College of London, London, UK ; Harefield Hospital, Royal Brompton and Harefield NHS Foundation Trust, Harefield, UK
Rees, Christiaan A; Geisel School of Medicine, Dartmouth College, Hanover, NH, USA
Nasir, Mavra; Geisel School of Medicine, Dartmouth College, Hanover, NH, USA
Thakuria, Louit; Harefield Hospital, Royal Brompton and Harefield NHS Foundation Trust, Harefield, UK
Simon, Andre; Harefield Hospital, Royal Brompton and Harefield NHS Foundation Trust, Harefield, UK
Reed, Anna K; Harefield Hospital, Royal Brompton and Harefield NHS Foundation Trust, Harefield, UK
Marczin, Nandor; Department of Surgery and Cancer, Section of Anaesthetics, Imperial College of London, London, UK ; Harefield Hospital, Royal Brompton and Harefield NHS Foundation Trust, Harefield, UK ; Department of Anesthesia and Intensive Care, Semmelweis University, Budapest, Hungary
Hill, Jane E; Thayer School of Engineering, Dartmouth College, Hanover, NH, USA. jane.hill@ubc.ca ; Geisel School of Medicine, Dartmouth College, Hanover, NH, USA. jane.hill@ubc.ca ; Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, Canada. jane.hill@ubc.ca
✱ These authors have contributed equally to this work.
Language :
English
Title :
Volatile organic compound profiling to explore primary graft dysfunction after lung transplantation.
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